[보고서]생태계교란 광엽식물 제거를 위한 천연 제초제 개발

최정섭

보고서 정보

주관연구기관

한국화학연구원
Korea Research Institute of Chemical Technology

연구책임자

최정섭

참여연구자

고영관 , 연규환 , 장경수 , 김영숙 , 김재덕 , 김혜진 , 곽화숙 , 이윤미

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2021-02

과제시작연도

2020

주관부처

농촌진흥청
Rural Development Administration(RDA)

과제관리전문기관

농촌진흥청
Rural Development Administration

등록번호

TRKO202100009923

과제고유번호

1395063689

사업명

생물다양성위협외래생물관리기술개발사업(R&D)(농진청)

DB 구축일자

2021-10-02

키워드

생태계교란 광엽식물.미생물 대사체.친환경 소재.저독성.제초활성.Invasive alien broad leaf plant.Microbial metabolites.Eco-friendly materials.Low toxicity.Herbicidal activity.

표 토양 분리 미생물배양액의 살초활성 평가 및 라이브러리 구축현황
표 KRA16-334균 배양액의 농도별 제초활성 평가
표 Herbicidal activity of N-29 culture broth filtrate on Sicyos angulatus with middle stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to S. angulatus with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate on Sicyos angulatus with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to S. angulatus with middle leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 10 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Ambrosia artemisiifolia with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to A. artemisiifolia with early leaf stage (2~3Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Ambrosia artemisiifolia with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to A. artemisiifolia with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Ambrosia artemisiifolia with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to A. artemisiifolia with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate of to Ambrosia trifida with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to A. trifida with early leaf stage (2~3Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Ambrosia trifida with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to A. trifida with late leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Ambrosia trifida with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to A. trifida with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Hypochaeris radicata with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to H. radicata with early leaf stage (2~3Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Hypochaeris radicata with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to H. radicata with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Hypochaeris radicata with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to H. radicata with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Solidago altissima with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to S. altissima with late leaf stage (2~3Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Solidago altissima with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to S. altissima with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Solidago altissima with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to S. altissima with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Sicyos angulatus in field condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to S. angulatus within field condition. The representative pictures were taken 20 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Ambrosia artemisiifolia in field condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to A. artemisiifolia within field condition. The representative pictures were taken 14 days after foliar application
표 Herbicidal activity of N-29 culture broth filtrate to Ambrosia trifida in field condition
표 Herbicidal activity of foliar application of N-29 culture broth filtrate to A. trifida within field condition. The representative pictures were taken 14 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Sicyos angulatus with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to S. angulatus with middle leaf stage (2~3Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Sicyos angulatus with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to S. angulatus with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Sicyos angulatus with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to S. angulatus with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Ambrosia trifida with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to A. trifida with middle leaf stage (2~3Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Ambrosia trifida with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to A. trifida with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Ambrosia trifida with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to A. trifida with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Ambrosia artemisiifolia with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to A. artemisiifolia with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Ambrosia artemisiifolia with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to A. artemisiifolia with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Ambrosia artemisiifolia with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to A. artemisiifolia with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Lactuca scariola with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to L. scariola with middle leaf stage (2~3Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Lactuca scariola with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to L. scariola with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Lactuca scariola with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to L. scariola with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Hypochaeris radicata with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to H. radicata with late leaf stage (2~3Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Hypochaeris radicata with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to H. radicata with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Hypochaeris radicata with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to H. radicata with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Solidago altissima with early leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to S. altissima with late leaf stage (2~3Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Solidago altissima with middle leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to S. altissima with middle leaf stage (4~5Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate on Solidago altissima with late leaf stage in a greenhouse condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to S. altissima with late leaf stage (7~8Ls) in a greenhouse condition. The representative pictures were taken 15 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Sicyos angulatus in field condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to S. angulatus in a greenhouse condition. The representative pictures were taken 20 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Ambrosia trifida in field condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to A. trifida in field condition. The representative pictures were taken 20 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Ambrosia artemisiifolia in field condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to A. artemisiifolia in field condition. The representative pictures were taken 20 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Hypochaeris radicata in field condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to H. radicata in field condition. The representative pictures were taken 20 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate to Solidago altissima in field condition
표 Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to S. altissima in field condition. The representative pictures were taken 20 days after foliar application
표 Purification and identification of herbicidal compounds from broth filtrate of KRA16-334
표 1D-, 2D-NMR spectrum and ESI-mass data of herbicidal compound 334-HP100-M
표 Chemical structure of herbicidal compound H70S70
표 Herbicidal activity of 334-HP100-M and HP50-W4
표 Herbicidal activity of 334-HP100-M and HP50-W4
표 Thermal stability of herbicidal compound HP50-W4
표 Condition for UV mutation of KRA16-334
표 Herbicidal activity of KRA16-334 mutants
표 Herbicidal activity and yield of active compounds of mutant M70 by culture temperature
표 Herbicidal activity and yield of active compounds of mutant M70 by agitation
표 Herbicidal activity and yield of active compounds of mutant M70 by initial pH
표 Herbicidal activity and yield of active compounds of KRA16-334 by culture
표 Herbicidal activity of N-29 culture broth filtrate by second followed-by treatment to several invasive alien plants in a greenhouse condition
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Sicyos angulatus in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Ambrosia artemisiifolia in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Ambrosia trifida in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Hypochaeris radicata in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Solidago altissima in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Lactuca scariola in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Herbicidal activity of KRA16-334 culture broth filtrate by second followed-by treatment to several invasive alien plants in a greenhouse condition
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Sicyos angulatus in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Ambrosia artemisiifolia in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Ambrosia trifida in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Hypochaeris radicata in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Solidago altissima in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Lactuca scariola in a greenhouse condition. The representative pictures were taken 14 days after foliar application
표 Herbicidal activity of culture broth filtrate N-29 and KRA16-334 by second followed-by treatment to several invasive alien plants in field condition
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Ambrosia artemisiifolia(left) and Hypochaeris radicata(right) in field condition. The representative pictures were taken 30 days after foliar application
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Solidago altissima in field condition. The representative pictures were taken 30 days after foliar application
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Ambrosia trifida in field condition. The representative pictures were taken 30 days after foliar application
표 Enhancement efficacy of N-29 culture broth filtrate by second followed-by treatment to Sicyos angulatus in field condition. The representative pictures were taken 30 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Hypochaeris radicata in field condition. The representative pictures were taken 30 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Solidago altissima in field condition. The representative pictures were taken 30 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Ambrosia trifida in field condition. The representative pictures were taken 30 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Ambrosia artemisiifolia in field condition. The representative pictures were taken 30 days after foliar application
표 Enhancement efficacy of KRA16-334 culture broth filtrate by second followed-by treatment to Sicyos angulatus in field condition. The representative pictures were taken 30 days after foliar application
표 Herbicidal enhancement efficacy of M-70 culture broth filtrate by Tween-20Ⓡ addition to Digitaria ciliaris and Solanum nigrum in a greenhouse condition
표 Herbicidal enhancement efficacy of broth filtrate by Tween-20 addition to Ditaria ciliaris and Solanum nigrum in a greenhouse condition
표 Herbicidal enhancement efficacy of M-70 broth filtrate by several adjuvants addition to Ditaria ciliaris and Solanum nigrum in a greenhouse condition
표 Herbicidal enhancement efficacy of M-70 broth filtrate by several adjuvants addition to Ditaria ciliaris and Solanum nigrum in a greenhouse condition. The representative pictures were taken 10 days after foliar application
표 Phytotoxicity of three adjuvants to Ditaria ciliaris in a greenhouse condition
표 Herbicidal activity of M-70 culture broth filtrate by three adjuvants addition to Ditaria ciliaris in a greenhouse condition
표 Herbicidal enhancement efficacy of M-70 culture broth filtrate by three adjuvants addition to Ditaria ciliaris in a greenhouse condition. The representative pictures were taken 4 days after foliar application
표 Kinetic solubility of KRA16-334 culture broth filtrate
표 The values of pH, DO, and temperature
표 Acute toxicity of herbicidin to D. magna
표 EC50 in acute toxicity of herbicidin to D. magna
표 Acute toxicity of herbicidin to O. latipes
표 Acute toxicity of herbicidin to C. carpio
표 Acute toxicity of herbicidin to M. anguillicaudatus
표 LC50 in acute toxicity of herbicdin to fish
표 Acute toxicity of herbicidin to E. fetida
표 Acute toxicity of herbicidin to E. fetida
표 Acute contact toxicity of herbicidin to A. mellifera
표 Acute oral toxicity of herbicidin to A. mellifera
표 Acute toxicity of herbicidin to A. mellifera
표 Number of dead or clinical abnormal female mice in acute oral toxicity experiments using herbicidin A
표 Body weight changes in female mice of acute oral toxicity experiments using herbicidin A
표 Number of dead or clinical abnormal female rats of acute dermal toxicity experiments using herbicidin A
표 Body weight changes in female rats of acute dermal toxicity experiments using herbicidin A
표 Clinical signs from the initial test in dermal irritation experiments on rabbits treated with herbicidin A
표 Clinical signs from the confirmatory tests in dermal irritation experiments on rabbits treated with herbicidin A
표 Grading of skin reactions (adapted from OECD TG No.404)
표 Body weight changes in female rabbits after a single dermal administration of herbicidin A
표 The score of eye mucosal reaction in rabbits
표 Grading of ocular lesions (adapted from OECD TG No.405)
표 Body weight changes in female rabbits after a single eye administration of herbicidin A
표 Mortalities and observed effects of Daphnia magna exposed to DHPA in the acute immobilisation test
표 Acute toxicity values of DHPA to Daphnia magna
표 Abnormal symptoms of O. latipes exposed to DHPA in acute toxicity test
표 Abnormal symptoms of C. capio exposed to DHPA in acute toxicity test
표 Abnormal symptoms of M. anguillicaudatus exposed to DHPA in acute toxicity test
표 Acute toxicity values of DHPA to O. latipes, C. capio, and M. anguillicaudatus
표 Mortalities and observed effects of Daphnia magna exposed to KRA 16-334 in the acute immobilisation test
표 Acute toxicity values of KRA 16-334 to Daphnia magna
표 Abnormal symptoms of O. latipes exposed to KRA 16-334 in acute toxicity test
표 Abnormal symptoms of C. capio exposed to KRA 16-334 in acute toxicity test
표 Abnormal symptoms of M. anguillicaudatus exposed to KRA 16-334 in acute toxicity test
표 Acute toxicity values of KRA 16-334 to O. latipes, C. capio, and M. anguillicaudatus
표 Abnormal symptoms and death of E. fetida exposed to KRA 16-334 in acute toxicity test
표 Acute toxicity of KRA 16-334 to E. fetida
표 Number of dead A. mellifera in acute oral toxicity test
표 Number of dead A. mellifera in acute contact toxicity test
표 Acute toxicity of KRA 16-334 to A. mellifera
표 Changes in germination rates of Ambrosia artemisiifolia and Ambrosia trifida seeds after harvest. Seeds were stored at room temperature (25℃) until use for experiment
표 Changes in germination rates of Lactuca serriola seeds after harvest. Seeds were stored at room temperature (25℃) until use for experiment
표 The effect of different constant-temperature regimes on seed germination of A. artemisiifolia, A. trifida and S angulatus
표 Effect of constant temperature on seed germination of Solidago altissima
표 The effect of photoperiod on seed germination of A. artemisiifolia, A. trifida and S. angulatus
표 The effect of different seed burial depths on seedling emergence rate of A. artemisiifolia, A. trifida and S. angulatus
표 The effect of different seed burial depths on plant height and shoot fresh weight of A. artemisiifolia, A. trifida and S. angulatus
표 Growth of A. artemisiifolia, A. trifida and S. angulatus under different seeding depth
표 Breaking the dormancy of Hypochaeris radicata seeds with H2SO4
표 Herbicidal effect of KRA16-334 on the emergence of Digitaria ciliaris in soil application 14 DAT under greenhouse condition
표 Growth inhibition of D. ciliaris at various rates of KRA16-334 under green house condition
표 Growth response of Digitaria ciliaris in the light and dark conditions after KRA16-334 treatments
표 Inhibition of photosynthesis in D. ciliaris by KRA16-334 and paraquat
표 Herbicidal symptoms and translocation of KRA16-334 at 3-4 leaf stage of Digitaria ciliaris
표 Herbicidal symptoms of KRA16-334 within 24 hours after treatment at 2-3 Leaf stage of Digitaria ciliaris
표 Electrolyte leakage in Cucumis sativus by KRA16-334 at different concentrations at 24 HAT

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생태계교란 광엽식물 제거를 위한 천연 제초제 개발
Development of natural herbicide for control of invasive alien broad leaf plant 원문보기

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생태계교란 광엽식물 제거를 위한 천연 제초제 개발 Development of natural herbicide for control of invasive alien broad leaf plant 원문보기

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생태계교란 광엽식물 제거를 위한 천연 제초제 개발
Development of natural herbicide for control of invasive alien broad leaf plant 원문보기

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