[보고서]고위험 바이러스 신속 진단을 위한 생체모방형 LSPR 나노센서 개발

새미사반

고위험 바이러스 신속 진단을 위한 생체모방형 LSPR 나노센서 개발
Biomimetic LSPR nanosensor for rapid diagnosis of lethal epidemic virus diseases 원문보기

보고서 정보
주관연구기관	성균관대학교 SungKyunKwan University
연구책임자	새미사반
보고서유형	최종보고서
발행국가	대한민국
언어	대한민국
발행년월	2021-06
과제시작연도	2021
주관부처	교육부 Ministry of Education
등록번호	TRKO202100012066
과제고유번호	1345344296
사업명	개인기초연구(교육부)(R&D)
DB 구축일자	2022-04-16
키워드	플라 스모 닉 센서.트롬빈 분석.구연산염 분석.알칼리성 포스파타제 분석.Plasmonic Sensor.Thrombin Assay.Citrate Assay.Alkaline phosphatase Assay.Aptamer.

초록

Abstract ▼

□ 연구개발 목표 및 내용
￭ 최종 목표 : The development of plasmonic sensor
￭ 전체 내용 : Providing plssmonic sensors platform for assaying different targets such as alkaline phophatase, citrate aminophenol, and thrombin
￭ 1단계(해당 시 작성)
• 목표 : Fabricating a solid sensor for thrombin assay based on LSPR shift.
• 내용 : A solid-phase colorimetric sensor to detect thrombin based on dual-aptamer sandwich has been provided and can visually detect a thrombin concentrationas low as 3μg/mL using dual-aptamer sandwich detection. The detection limit of the dual assay was 1.33μg/mL, which was three times lower than that of the single apta-sensor, showing improved sensitivity and detection efficiency with excellent specificity and clinical applicability in real human serum.
￭ 2단계(해당 시 작성)
• 목표 : Fabricating a colorimetric sensors platform for assaying citrate
• 내용 : We successfully provided a plssmonic colorimetric sensors platform for assaying citrate with highly selectivity and sensitivity which exhibits a visual detection limit of 25 μM by naked eye readout and low limit of 4.05 nMby UV-Vis measurements, which is the best detection sensitivity of citrate in the literature. The The developed sensor can be used asaffordable and portable on-site kit for citrate detection without complicated protocols on filter paper, with a satisfactory low limit of detection of 10.1 μM by UV-visspectrometer and a low naked-eye detection of 250 μM with high specifity and clinical applicability in real urine samples
￭ 3단계(해당 시 작성)
• 목표 : Fabricating a plssmonic colorimetric sensor platform for assaying of alkaline phosphatase
• 내용 : We successfully provided a plssmonic colorimetric sensors platform for assaying of alkaline phosphatase a potenial biomarker for many diseases and dental inflamations. We developed a colorimetric ALP biosensor based on the pAP-mediated growth of AgNPs with high precision and high reproducibility. We used pAPP as a new substrate for the dephosphorylation process by ALP to produce pAP for further growth of AgNPs showing a linear dynamic range as wide as 0.5–225 U/L and LOD as low as 0.24 U/L.

□ 연구개발성과
We successfully provided a plssmonic sensors platform for assaying different targets such as alkaline phophatase, citrate, aminophenol and thrombin.

□ 연구개발성과 활용계획 및 기대 효과
However we provided some colorimetric plasmonic sensor for different targets as alakaline phosphatase, aminophenol, citrate and thrombin, the plasmonic sensor sttill need more development regarding the stability and selectivity as well as know-how to tranfer the technology to industrial board.

(출처 : 요약문 2p)

목차 Contents

COVER ... 1
요약문 ... 2
목차 ... 3
1. 연구개발과제의 개요 ... 4
2. 연구개발과제의 수행 과정 및 수행 내용 ... 4
3. 연구개발과제의 수행 결과 및 목표 달성 정도 ... 5
1) 연구수행 결과 ... 5
4. 연구개발성과의 관련 분야에 대한 기여 정도 ... 29
5. 연구개발성과의 관리 및 활용 계획 ... 29
6. 참고문헌 ... 29
End of Page ... 29

표/그림 (28)

표 Schematic diagram of the fabricated solid-phase apta-biosensor for thrombin detection. The first step is the incubation of thrombin with the fabricated AuNPTBA1 solid biosensor, and the second step is the conjugation of the prepared AuNP-TBA2 solution with the AuNP-TBA1-thrombin complex for amplification of the LSPR signal. The scanning electron microscopic images demonstrate the aggregation induced by thrombin incubation, inducing peak enhancement and red-shift as observed in the UV spectrum
표 UV–vis spectra of (A) AuNPs conjugated with TBA1 on the APTES-coated substrate and (B) AuNPs conjugated with TBA2 in solution (red-shift is observed upon binding with TBA2
표 UV–vis spectra of (A) the first sensing stage of AuNP-TBA1-thrombin and (B) dual sensing platform AuNP-TBA1-thrombin-TBA2-AuNPs at various thrombin concentrations. (C) Images of single and dual apta-sensor with different thrombin concentrations. (D) Calibration curves of single and dual apta-sensor
표 (A) Specificity of the developed solid-phase dual apta-biosensor for the detection of various interfering analytes (IgG, OPN, BSA, and complex mixture of analytes at a concentration of 10 μg/mL). (B) Linear relationship between the peak shift of the prepared biosensor at 520 nm and thrombin concentration (1–10 μg/ mL) spiked in the serum sample
표 Schematic representation of the citrate-induced AgNP aggregation of the prepared dual AgNP sensor
표 (a) UV−vis spectra of the dual AgNP sensor probe for varying amounts of citrate anions in distilled water, (b) the corresponding standard calibration, and (c) photos demonstrating the visual detection of citrate
표 UV−vis response of the dual-AgNP sensor with (a) various metal cations (the inset figure refers to color selectivity) and (b) various anions (the inset figure refers to color selectivity). (c) UV−vis response of the dual-AgNP sensor with different concentrations of citrate in tap water (the inset figure refers to the color change with citrate in the copresence of different cations and anions present in tap water) and (d) the corresponding linear calibration curve between Δλ530 and the citrate concentration (n = 3) in tap water
표 (a) UV−vis spectra of the paper-based dual-AgNP sensor with different concentrations of citrate (10−1000 μM), (b) linear calibration between Δλ585 and the citrate concentration, and (c) photo demonstrating the visual detection of citrate using paper-based dual-AgNPs
표 (a) UV−vis spectra of the dual-AgNP sensor with different amounts of spiked citrate in urine (concentration of the zero-spiked sample:11.4 μM), (b) the linear standard calibration change at Δλ530 of the dual-AgNP sensor, and (c) photo of the visual detection of citrate in urine
표 Analytical results of citrate detection in urine using the Dual-AgNP sensor
표 Reaction pathway of the multi-carbonyl Gemini cationic surfactants, CACB, LACB and PACB
표 (a) FTIR and (b) 1HNMR spectrum of freshly prepared Gemini cationic amphiphilic LACB
$(a)-(g) AgNPs characterization: (a)-(c) TEM images of synthesized AgNPs capped with synthesized Gemini cationic amphiphilic CACB, LACB & PACB, respectively (insets are the corresponding selected area electron diffraction pattern). (d)-(f) Size distributions of AgNPs capped with CACB, LACB and PACB, respectively. (g) UV-Vis spectra of AgNPs capped with CACB, LACB and PACB, respectively$ 표 (a)-(g) AgNPs characterization: (a)-(c) TEM images of synthesized AgNPs capped with synthesized Gemini cationic amphiphilic CACB, LACB & PACB, respectively (insets are the corresponding selected area electron diffraction pattern). (d)-(f) Size distributions of AgNPs capped with CACB, LACB and PACB, respectively. (g) UV-Vis spectra of AgNPs capped with CACB, LACB and PACB, respectively
표 (a)-(c) The relationship between surface tension-log [C] of prepared CACB, LACB and PACB surfactants, respectively, at various temperatures. (d) Effect of both temperature & hydrocarbon tail length on critical micelle concentration
표 Thermodynamic insights of micellization and adsorption processes of the amphiphilic CACB, LACB and PACB
표 Emulsion, foam and interfacial performance of the prepared Gemini cationic amphiphilic at 25 ℃
표 Scheme 1. Schematic representation of ALP detection based on the pAP-mediated growth of AgNPs
표 Optimization of the ALP assay conditions. Effect of the (a) initial pAPP substrate concentration (b) pH of the medium and (c) incubation time were investigated
표 (a) UV–vis spectra of the colorimetric ALP biosensor based on the pAP-mediated growth of AgNPs, with different concentrations of ALP and corresponding calibration curve showing the change in the absorbance of the biosensor at a wavelength of (b) 407, and (c) 520 nm as a function of ALP concentration. (d) Photograph of the color change of the sensor with different concentrations of ALP. The concentrations of ALP are 0, 0.5, 1.25, 2.5, 5, 7.5, 12.5, 17.5, 25, 30, 37.5, 50, 62.5, 75, 100, 125, 150, 175, 200, and 225 U/L
표 (a) Selectivity test of the ALP biosensor with the seven common biomolecules and ALP. (b) Interference test of the ALP biosensor in the presence of the biomolecules and ALP. The concentrations of ALP in the selectivity and interference test were 150 and 100 U/L respectively, whereas the concentrations of BSA, MMP9, penicillin, thrombin, GOX, trypsin, and IgG were 100 μg/mL, 0.2 μg/mL, 500 U/L, 0.5 μg/mL, 50 μg/mL, 100 μg/mL, and 50 μg/mL, respectively
표 (a) UV–vis spectra of the ALP biosensor with different concentrations of the added ALP in a 10 % diluted human serum solution and (b) linear standard addition calibration curve of the change in the absorbance of the biosensor as a function of added ALP concentration
표 Analytical results of the ALP assay in the 10 % diluted human serum solution using the standard addition method
표 Synthetic route of water-soluble non-ionic surfactant
표 (a) FTIR, (b) 1HNMR spectrum of the synthesized DMAC non-ionic surface active agents
표 The relationship between surface tension and log concentration of DMAC, DMAP andDMAO non-ionic surfactant, respectively, at various temperatures; (d) Temperatures and hydrophobic tail length effect of the synthesized DMAC, DMAP and DMAO non-ionic Surfactant
표 (a-c) Typical anodic polarization curve for electropolishing of vertical C-steel plate in 8 M H3PO4 in presence of different concentrations of DMAC, DMAP and DMAO at 15 °C.(d) Langmuir adsorption isotherm for C-steel in 8 M H3PO4 solution at 15 °C for studied surfactant
표 (a) Inhibition efficiency percentage – concentration dependence curves for C-steel in 8 M H3PO4 solution contaning different surfactant at 15 °C and 10 h. (b) Inhibition efficiency percentage – immersion time dependence curves for C-steel in 8 M H3PO4 solution contaning different surfactants at 15 °C
표 SEM of the surface morphology before and after EP at 288 °K in the absence and presence of synthesized nonionic surfactant

과제명(ProjectTitle) :	-
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연구목표(Goal) :	-
연구내용(Abstract) :	-
기대효과(Effect) :	-

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연합인증

고위험 바이러스 신속 진단을 위한 생체모방형 LSPR 나노센서 개발
Biomimetic LSPR nanosensor for rapid diagnosis of lethal epidemic virus diseases 원문보기