![Mechanism of boronic ester cleavage by hydrogen peroxide derived from activated photosensitizer (a) and ROS-triggered drug release from CSPM-PheoA-5′-DFCR hydrogel analyzed by HPLC (b). The hydrogel samples (5 mg/mL) in DMSO were exposed to NIR laser (λ = 670 nm, 167 mW/cm2, irradiation time = 1, 2, 3, 4, 5, 10, and 30 min, respectively). After centrifugation of the samples at 13,000 rpm for 30 min, supernatants were collected and the amount of free 5′-DFCR in each sample was measured by HPLC [injection volume = 10 μL; elution buffer = DW/Methanol (8:2, v/v), absorbance at 220 nm, retention time = 4.15 min]](https://nrms.kisti.re.kr/bitextimages/TRKO202100013928/TRKO202100013928_15_image_1.png "Mechanism of boronic ester cleavage by hydrogen peroxide derived from activated photosensitizer (a) and ROS-triggered drug release from CSPM-PheoA-5′-DFCR hydrogel analyzed by HPLC (b). The hydrogel samples (5 mg/mL) in DMSO were exposed to NIR laser (λ = 670 nm, 167 mW/cm2, irradiation time = 1, 2, 3, 4, 5, 10, and 30 min, respectively). After centrifugation of the samples at 13,000 rpm for 30 min, supernatants were collected and the amount of free 5′-DFCR in each sample was measured by HPLC [injection volume = 10 μL; elution buffer = DW/Methanol (8:2, v/v), absorbance at 220 nm, retention time = 4.15 min]")
![Concentration-dependent cellular toxicity of CSPM and CSPM-PheoA hydrogel and NIR-induced PDT effect. (a) PANC-1 cells were treated with hydrogels and cell viability test (WST-1 assay) was performed for three days. To evaluate the PDT effect of the photosensitizer PheoA in hydrogels, the cells were treated with CSPM-PheoA and CSPM-PheoA-5′-DFCR in DMSO (final concentration 65 μg/mL, 5% DMSO in medium) and exposed to NIR laser [λ = 671 nm, 100 J/cm2, NIR(+) or NIR(-)]. After 2 h of incubation, cell viability was measured using a UV-Vis spectrometer (tested individual sample set, n=5., ***P<0.001 by student t-test)](https://nrms.kisti.re.kr/bitextimages/TRKO202100013928/TRKO202100013928_21_image_1.png "Concentration-dependent cellular toxicity of CSPM and CSPM-PheoA hydrogel and NIR-induced PDT effect. (a) PANC-1 cells were treated with hydrogels and cell viability test (WST-1 assay) was performed for three days. To evaluate the PDT effect of the photosensitizer PheoA in hydrogels, the cells were treated with CSPM-PheoA and CSPM-PheoA-5′-DFCR in DMSO (final concentration 65 μg/mL, 5% DMSO in medium) and exposed to NIR laser [λ = 671 nm, 100 J/cm2, NIR(+) or NIR(-)]. After 2 h of incubation, cell viability was measured using a UV-Vis spectrometer (tested individual sample set, n=5., ***P<0.001 by student t-test)")
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| 주관연구기관 | 인하대학교 InHa University |
|---|---|
| 연구책임자 | 이정한 |
| 보고서유형 | 최종보고서 |
| 발행국가 | 대한민국 |
| 언어 | 한국어 |
| 발행년월 | 2020-06 |
| 과제시작연도 | 2020 |
| 주관부처 | 과학기술정보통신부 Ministry of Science and ICT |
| 연구관리전문기관 | 한국연구재단 National Research Foundation of Korea |
| 등록번호 | TRKO202100013928 |
| 과제고유번호 | 1345314374 |
| 사업명 | 개인기초연구(교육부)(R&D) |
| DB 구축일자 | 2021-09-18 |
| 키워드 | 대장암.나노입자.광역학치료.광 분해.광 영상.약물방출.내시경.영상가이드.암줄기세포. |
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