![RT-PCR of pmrC transcript and lipid A analysis. (A) Expression levels of pmrC relative to 16S rRNA genes at the sampling time of 3 h during cultivation of the three strains CAM121, KCTC 12416, and KCTC 12406. Statistically significant differences between the DDCt values of the strains were determined by two-tailed t-tests with P values of less than 0.05. n = 4 independent experiments. (B) A silica gel thin-layer chromatography of [32P] orthophosphate-labeled lipid A extracts from the three strains. E. coli strain W3110 was included as the reference strain for identification of mono-, bis-, and tri-phosphorylated lipid A positions. The position of phosphoethanolamine (pEtN)-modified lipid A is shown by an arrow at the right of the gel. (C) MALDI-TOF mass spectra of lipid A extracts obtained from the three strains treated (+) or untreated (-) with 10 mg/mL colistin for 1 h at the time of 3 h during cultivation in 10 mL LB broth with aeration at 37 ºC](https://nrms.kisti.re.kr/bitextimages/TRKO202100017443/TRKO202100017443_10_image_2.png "RT-PCR of pmrC transcript and lipid A analysis. (A) Expression levels of pmrC relative to 16S rRNA genes at the sampling time of 3 h during cultivation of the three strains CAM121, KCTC 12416, and KCTC 12406. Statistically significant differences between the DDCt values of the strains were determined by two-tailed t-tests with P values of less than 0.05. n = 4 independent experiments. (B) A silica gel thin-layer chromatography of [32P] orthophosphate-labeled lipid A extracts from the three strains. E. coli strain W3110 was included as the reference strain for identification of mono-, bis-, and tri-phosphorylated lipid A positions. The position of phosphoethanolamine (pEtN)-modified lipid A is shown by an arrow at the right of the gel. (C) MALDI-TOF mass spectra of lipid A extracts obtained from the three strains treated (+) or untreated (-) with 10 mg/mL colistin for 1 h at the time of 3 h during cultivation in 10 mL LB broth with aeration at 37 ºC")
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| 주관연구기관 | 대구가톨릭대학교 Catholic University of Daegu |
|---|---|
| 연구책임자 | 김용학 |
| 보고서유형 | 최종보고서 |
| 발행국가 | 대한민국 |
| 언어 | 한국어 |
| 발행년월 | 2021-03 |
| 과제시작연도 | 2020 |
| 주관부처 | 과학기술정보통신부 Ministry of Science and ICT |
| 과제관리전문기관 | 한국연구재단 National Research Foundation of Korea |
| 등록번호 | TRKO202100017443 |
| 과제고유번호 | 1711107784 |
| 사업명 | 개인기초연구(과기정통부)(R&D) |
| DB 구축일자 | 2022-02-19 |
| 키워드 | 아시네토박터.콜리스틴 내성.지질다당류.pmrCAB 오페론.키메라 단백질 유전자.Acinetobacter.Colistin resistance.Lipooligosacch aride.pmrCAB operon.Chimeric protein-coding gene. |
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