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Application of digital polymerase chain reaction technology for noninvasive prenatal test 원문보기

Journal of genetic medicine, v.12 no.2, 2015년, pp.72 - 78  

Lee, Seung Yong (Bio-Core Co. Ltd.) ,  Hwang, Seung Yong (Bio-Core Co. Ltd.)

Abstract AI-Helper 아이콘AI-Helper

Recently, noninvasive prenatal test (NIPT) has been adopted as a primary screening tool for fetal chromosomal aneuploidy. The principle of NIPT lies in isolating the fetal fraction of cell-free DNA in maternal plasma and analyzing it with bioinformatic tools to measure the amount of gene from the ta...

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제안 방법

  • The chip-based platform consists in reaction preparation and automatic loading of the reaction mixture to the chip where the PCR reaction will be carried out in each well of the chip plate. The amplified reaction is then analyzed by counting the wells, followed by statistical analysis. On the basis of the results of analysis, it can be applied to various methods (Fig.
  • [30] introduced dPCR for screening fetal T21 in 2007. The authors demonstrated the feasibility of dPCR in determining the allelic imbalance of a single nucleotide polymorphism in PLAC4 mRNA of chromosome 21. In addition, they detected T21 prenatally by using a nonpolymorphism-based approach with dPCR.
  • [32] conducted power analysis. The authors developed a power equation and calculated the number of wells that were important for the experimental cost. With the model that was adopted in the study, 2,609 wells would be sufficient to achieve a 99% detection rate with a 1% false-positive rate.
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참고문헌 (40)

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