Over the past decades, a considerable number of studies has been conducted on new means. But fundamental questions remain unanswered. The largest problem in extract is the strong tissue of seaweed. The purpose of this study is screening of microfloras involved in hydrolysis of sea tangle, sea mustar...
Over the past decades, a considerable number of studies has been conducted on new means. But fundamental questions remain unanswered. The largest problem in extract is the strong tissue of seaweed. The purpose of this study is screening of microfloras involved in hydrolysis of sea tangle, sea mustard, seaweed tenella, seaweed fusiforme and green laver. It is difficult to extract the useful constituent parts from seaweed as intercellular mucilage, storage polysaccharide and mineral. Accordingly, the purpose of this paper is to hydrolyze the seaweed by using microorganisms. First, about two hundred microflora samples were obtained from mountain, rice field, dry field, sea, seaside and fish market in the vicinity of Yeosu. Thirty-three microflora samples were screened from the destruction of tissue in sea tangle and sea mustard. It was sufficient that results of the naked eye observation were obtained at eight microflora samples as a feces of bull, a decayed pine tree, a soil of dry field, the mud of the banks in a rice field, the water of a ditch in a rice field, the weed of the banks in a rice field, the water in a rice field and leaved in the air. Sodium alginate 0.5%, bacto-peptone 0.5%, NaCl 1.0%, pH 7.5, incubation temperature 25±1℃ and 72 hours were optimum conditions for the incubation of 08B121 strain. Sodium alginate 0.7%, bacto-peptone 0.3%, NaCI 1.0%, pH 7.0, incubation temperature 25±1℃ and 120 hours were optimum conditions for the incubation of 27A111 strain. Sodium alginate 0.7%, bacto-peptone 0.7%, NaCI 0.7%, pH 7.0, incubation temperature 25±1℃ and 72 hours were optimum conditions for the incubation of 27A311 strain. Sodium alginate 0.7%, bacto-peptone 0.3%, NaCI 1.2%, pH 7.0, incubation temperature 25±1℃ and 72 hours were optimum conditions for the incubation of 27B121 strain. And sodium alginate 0.7%, bacto-peptone 0.3%, NaCI 10.0%, pH 7.5, incubation temperature 35±1℃ and 72 hours were optimum conditions for the incubation of 27C221 strain. The 08B121 strain was identified with Bacillus sp. from biochemical and biological experiment ; gram positive, motility positive, oxidase test negative, catalase test positive, rods shape, aerobic etc. The 27A111, 27A311 and 27B121 strains were identified with Pseudomonas sp. from biochemical and biological experiment ; gram negative, motility positive, oxidase test positive, catalase test positive, rods shape, aerobic etc. The 27C221 strain was identified with Halobacterium sp. from biochemical and biological experiment ; gram negative, motility negative, oxidase test positive, catalase test positive, rods shape, aerobic etc. The optimum temperature, time and pH for the extracellular crude enzymes activity were 35∼40℃, 40∼45 minutes and 6∼8, respectively. The optimum temperature, time and pH for the intracellular crude enzymes activity were 25∼50℃, 35∼40 minutes and 6∼8, respectively. The extracellular crude enzymes were stable at pH values ranging from 6.0 to 8.0 and preincubation temperature ranging from 0℃ to 40℃. The intracellular crude enzymes were stable at pH values ranging from 5.5 to 8.5 and preincubation temperature ranging from 0℃ to 45℃. Solid yields of sea tangle and sea mustard hydrolysates hydrolyzed by extracellular crude enzymes of 5 strains were respectively 1.38∼1.59g/100ml and 1.53∼1.60g/100ml. And solids yields of sea tangle and sea mustard hydrolysates hydrolyzed by intracellular crude enzymes of 5 strains were respectively 1.32 ∼1.70g/100ml and 1.52∼1.54g/100ml. Proximate compositions of sea tangle hydrolysates hydrolyzed by extracellular crude enzymes of 5 strains were as follows ; moisture 98.41~98.62%, crude protein 0.08∼0.10%, crude lipid 0.19∼0.34%, crude ash 0.37∼0.43%, total sugar 583.14∼872.62mg%, reducing sugar 123.91∼197.72mg%, L-value 75.00∼78.27, a-value -0.83∼-0.41 and b-value 13.57∼15.67. Proximate compositions of sea tangle hydrolysates hydrolyzed by intracellular crude enzymes of 5 strains were as follows ; moisture 98.30∼98.68%, crude protein 0.07∼0.11%, crude lipid 0.18∼0.30%, crude ash 0.33~0.42%, total sugar 594.59∼948.29mg%, reducing sugar 125.38∼218.48mg%, L-value 76.39∼87.13, a-value -1.20∼-0.63 and b-value 11.31∼16.13. Proximate compositions of sea mustard hydrolysates hydrolyzed by extracellular crude enzymes of 5 strains were as follows ; moisture 98.40∼98.47%, crude protein 0.11∼0.13%, crude lipid 0.24∼0.32%, crude ash 0.29∼0.36%, total sugar 804.27∼882.28mg%, reducing sugar 225.25∼279.73mg%, L-value 76.75∼86.15, a-value -7.57∼-6.77 and b-value 27.03∼29.12. Proximate compositions of sea mustard hydrolysates hydrolyzed by intracellular crude enzymes of 5 strains were as follows ; moisture 98.46∼98.48%, crude protein 0.11∼0.13%, crude lipid 0.23∼0.28%, crude ash 0.43∼0.46%, total sugar 659.73∼767.66mg%, reducing sugar 196.37∼247.28mg%, L-value 78.38∼89.23, a-value -8.56∼-7.90 and b-value 27.48∼31.59. The results of this experiment will change the direction of the entire industry of seaweeds. The effect of research may be summarized as follows: an increase in value added of seaweeds, the improvement of acceptability, functional and storage stability, a considerable extension of one's business, an accumulation of know-how in micoorganisms and hydrolases technology, the exportation of products and technology, the contribution largely to processing of seaweeds and production of various seaweeds products, and especially the standardization of process is the key to solve all of these problems. This document is applied to all of areas.
Over the past decades, a considerable number of studies has been conducted on new means. But fundamental questions remain unanswered. The largest problem in extract is the strong tissue of seaweed. The purpose of this study is screening of microfloras involved in hydrolysis of sea tangle, sea mustard, seaweed tenella, seaweed fusiforme and green laver. It is difficult to extract the useful constituent parts from seaweed as intercellular mucilage, storage polysaccharide and mineral. Accordingly, the purpose of this paper is to hydrolyze the seaweed by using microorganisms. First, about two hundred microflora samples were obtained from mountain, rice field, dry field, sea, seaside and fish market in the vicinity of Yeosu. Thirty-three microflora samples were screened from the destruction of tissue in sea tangle and sea mustard. It was sufficient that results of the naked eye observation were obtained at eight microflora samples as a feces of bull, a decayed pine tree, a soil of dry field, the mud of the banks in a rice field, the water of a ditch in a rice field, the weed of the banks in a rice field, the water in a rice field and leaved in the air. Sodium alginate 0.5%, bacto-peptone 0.5%, NaCl 1.0%, pH 7.5, incubation temperature 25±1℃ and 72 hours were optimum conditions for the incubation of 08B121 strain. Sodium alginate 0.7%, bacto-peptone 0.3%, NaCI 1.0%, pH 7.0, incubation temperature 25±1℃ and 120 hours were optimum conditions for the incubation of 27A111 strain. Sodium alginate 0.7%, bacto-peptone 0.7%, NaCI 0.7%, pH 7.0, incubation temperature 25±1℃ and 72 hours were optimum conditions for the incubation of 27A311 strain. Sodium alginate 0.7%, bacto-peptone 0.3%, NaCI 1.2%, pH 7.0, incubation temperature 25±1℃ and 72 hours were optimum conditions for the incubation of 27B121 strain. And sodium alginate 0.7%, bacto-peptone 0.3%, NaCI 10.0%, pH 7.5, incubation temperature 35±1℃ and 72 hours were optimum conditions for the incubation of 27C221 strain. The 08B121 strain was identified with Bacillus sp. from biochemical and biological experiment ; gram positive, motility positive, oxidase test negative, catalase test positive, rods shape, aerobic etc. The 27A111, 27A311 and 27B121 strains were identified with Pseudomonas sp. from biochemical and biological experiment ; gram negative, motility positive, oxidase test positive, catalase test positive, rods shape, aerobic etc. The 27C221 strain was identified with Halobacterium sp. from biochemical and biological experiment ; gram negative, motility negative, oxidase test positive, catalase test positive, rods shape, aerobic etc. The optimum temperature, time and pH for the extracellular crude enzymes activity were 35∼40℃, 40∼45 minutes and 6∼8, respectively. The optimum temperature, time and pH for the intracellular crude enzymes activity were 25∼50℃, 35∼40 minutes and 6∼8, respectively. The extracellular crude enzymes were stable at pH values ranging from 6.0 to 8.0 and preincubation temperature ranging from 0℃ to 40℃. The intracellular crude enzymes were stable at pH values ranging from 5.5 to 8.5 and preincubation temperature ranging from 0℃ to 45℃. Solid yields of sea tangle and sea mustard hydrolysates hydrolyzed by extracellular crude enzymes of 5 strains were respectively 1.38∼1.59g/100ml and 1.53∼1.60g/100ml. And solids yields of sea tangle and sea mustard hydrolysates hydrolyzed by intracellular crude enzymes of 5 strains were respectively 1.32 ∼1.70g/100ml and 1.52∼1.54g/100ml. Proximate compositions of sea tangle hydrolysates hydrolyzed by extracellular crude enzymes of 5 strains were as follows ; moisture 98.41~98.62%, crude protein 0.08∼0.10%, crude lipid 0.19∼0.34%, crude ash 0.37∼0.43%, total sugar 583.14∼872.62mg%, reducing sugar 123.91∼197.72mg%, L-value 75.00∼78.27, a-value -0.83∼-0.41 and b-value 13.57∼15.67. Proximate compositions of sea tangle hydrolysates hydrolyzed by intracellular crude enzymes of 5 strains were as follows ; moisture 98.30∼98.68%, crude protein 0.07∼0.11%, crude lipid 0.18∼0.30%, crude ash 0.33~0.42%, total sugar 594.59∼948.29mg%, reducing sugar 125.38∼218.48mg%, L-value 76.39∼87.13, a-value -1.20∼-0.63 and b-value 11.31∼16.13. Proximate compositions of sea mustard hydrolysates hydrolyzed by extracellular crude enzymes of 5 strains were as follows ; moisture 98.40∼98.47%, crude protein 0.11∼0.13%, crude lipid 0.24∼0.32%, crude ash 0.29∼0.36%, total sugar 804.27∼882.28mg%, reducing sugar 225.25∼279.73mg%, L-value 76.75∼86.15, a-value -7.57∼-6.77 and b-value 27.03∼29.12. Proximate compositions of sea mustard hydrolysates hydrolyzed by intracellular crude enzymes of 5 strains were as follows ; moisture 98.46∼98.48%, crude protein 0.11∼0.13%, crude lipid 0.23∼0.28%, crude ash 0.43∼0.46%, total sugar 659.73∼767.66mg%, reducing sugar 196.37∼247.28mg%, L-value 78.38∼89.23, a-value -8.56∼-7.90 and b-value 27.48∼31.59. The results of this experiment will change the direction of the entire industry of seaweeds. The effect of research may be summarized as follows: an increase in value added of seaweeds, the improvement of acceptability, functional and storage stability, a considerable extension of one's business, an accumulation of know-how in micoorganisms and hydrolases technology, the exportation of products and technology, the contribution largely to processing of seaweeds and production of various seaweeds products, and especially the standardization of process is the key to solve all of these problems. This document is applied to all of areas.
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