개망초의 엽과 줄기, 꽃에 함유된 제초활성물질을 탐색하기 위하여 methanol 추출물의 분획물에 대한 상추종자의 생물검정을 통하여 제초활성 물질들을 분리, 정제한 다음 화합물들의 구조분석과 활성을 조사한 결과는 다음과 같다. 1. 개망초의 엽·줄기 그리고 꽃의 용매 분획별 추출물을 생물검정한 결과, 모두 5000ppm의 EtOAc 추출물에서 강한 발아억제활성을 보였다. 2. EtOAc 분획물로부터 제초활성물질을 분리·동정한 결과, 엽·줄기에서는 compound 1(GMCLEA-3.2.2)과 compound 2(GMCLEA-3.2.5)가 강한 제초활성을 보였으며, 꽃에서는 compound 3(GMCLEA-1)과 compound 6(GMCFEA-2.3.6)이 강한 제초활성을 보였다. 3. 잎·줄기에서 분리된 화합물의 구조분석결과 compound 1은 4-hydroxy-cinnamic acid로 compound 2는 3,4-dihydroxy ...
개망초의 엽과 줄기, 꽃에 함유된 제초활성물질을 탐색하기 위하여 methanol 추출물의 분획물에 대한 상추종자의 생물검정을 통하여 제초활성 물질들을 분리, 정제한 다음 화합물들의 구조분석과 활성을 조사한 결과는 다음과 같다. 1. 개망초의 엽·줄기 그리고 꽃의 용매 분획별 추출물을 생물검정한 결과, 모두 5000ppm의 EtOAc 추출물에서 강한 발아억제활성을 보였다. 2. EtOAc 분획물로부터 제초활성물질을 분리·동정한 결과, 엽·줄기에서는 compound 1(GMCLEA-3.2.2)과 compound 2(GMCLEA-3.2.5)가 강한 제초활성을 보였으며, 꽃에서는 compound 3(GMCLEA-1)과 compound 6(GMCFEA-2.3.6)이 강한 제초활성을 보였다. 3. 잎·줄기에서 분리된 화합물의 구조분석결과 compound 1은 4-hydroxy-cinnamic acid로 compound 2는 3,4-dihydroxy cinnamic acid methyl ester로 동정되었으며, 꽃으로부터 분리된 compound 3은 3-hydroxy-pyran-4-one으로 compound 6은 (5-butyl-3-oxo-2,3-dihydro-furan-2-yl)-acetic acid로 동정되었는데, 이 중 compound 6은 신물질로 확인되었다. 4. 꽃에서 분리된 분획물 중 caffeic acid가 2중결합된 compound 4(3,5-Di-O-caffeoylquinic acid)와 5(methyl 3,5-Di-O-caffeoylquinate)는 제초활성을 보이지 않았으나, 잎·즐기에서 추출된 caffeic acid에 methyl 기가 결합된 compound 2(3,4-dihydroxycinnamic acid methyl ester)는 제초활성을 보였다. 5. 제초활성을 보인 4종의 화합물에 대한 활성을 IC_(50)으로 비교한 결과, compound 1은 816ppm, compound 2는 945ppm, compound 3은 1440ppm, 그리고 compound 6은 421ppm 이었으며, 시약용 4-hydroxybenzoic acid의 IC_(50)은 555ppm으로 compound 6은 이보다 좋은 활성을 나타내었다.
개망초의 엽과 줄기, 꽃에 함유된 제초활성물질을 탐색하기 위하여 methanol 추출물의 분획물에 대한 상추종자의 생물검정을 통하여 제초활성 물질들을 분리, 정제한 다음 화합물들의 구조분석과 활성을 조사한 결과는 다음과 같다. 1. 개망초의 엽·줄기 그리고 꽃의 용매 분획별 추출물을 생물검정한 결과, 모두 5000ppm의 EtOAc 추출물에서 강한 발아억제활성을 보였다. 2. EtOAc 분획물로부터 제초활성물질을 분리·동정한 결과, 엽·줄기에서는 compound 1(GMCLEA-3.2.2)과 compound 2(GMCLEA-3.2.5)가 강한 제초활성을 보였으며, 꽃에서는 compound 3(GMCLEA-1)과 compound 6(GMCFEA-2.3.6)이 강한 제초활성을 보였다. 3. 잎·줄기에서 분리된 화합물의 구조분석결과 compound 1은 4-hydroxy-cinnamic acid로 compound 2는 3,4-dihydroxy cinnamic acid methyl ester로 동정되었으며, 꽃으로부터 분리된 compound 3은 3-hydroxy-pyran-4-one으로 compound 6은 (5-butyl-3-oxo-2,3-dihydro-furan-2-yl)-acetic acid로 동정되었는데, 이 중 compound 6은 신물질로 확인되었다. 4. 꽃에서 분리된 분획물 중 caffeic acid가 2중결합된 compound 4(3,5-Di-O-caffeoylquinic acid)와 5(methyl 3,5-Di-O-caffeoylquinate)는 제초활성을 보이지 않았으나, 잎·즐기에서 추출된 caffeic acid에 methyl 기가 결합된 compound 2(3,4-dihydroxycinnamic acid methyl ester)는 제초활성을 보였다. 5. 제초활성을 보인 4종의 화합물에 대한 활성을 IC_(50)으로 비교한 결과, compound 1은 816ppm, compound 2는 945ppm, compound 3은 1440ppm, 그리고 compound 6은 421ppm 이었으며, 시약용 4-hydroxybenzoic acid의 IC_(50)은 555ppm으로 compound 6은 이보다 좋은 활성을 나타내었다.
In the searching for the secondary metabolites with inhibitory effects on the germination of lettuce seeds, the methanol extracts from the leaves, stems, and flowers of Erigeron annuus were screened for their inhibitory effects, and the investigations of the active principles of the extracts were co...
In the searching for the secondary metabolites with inhibitory effects on the germination of lettuce seeds, the methanol extracts from the leaves, stems, and flowers of Erigeron annuus were screened for their inhibitory effects, and the investigations of the active principles of the extracts were conducted by bioassay-guided fractionation. The summary of the study is as follows: 1. Methanol extracts from the leaves, stems, and flowers Erigeron annuus were concentrated, and suspended in water, and sequentially partitioned with n-hexane, EtOAc, and BuOH. Each fraction was tested for their activity, and EtOAc-soluble fractions from the leaves, stems, and flowers Erigeron annuus exhibited strong inhibitory effects at 5000 ppm level. 2. The above active EtOAc-soluble extracts were further fractionated on the basis of the bioassay results. From the extracts of the leaves and stems, compound 1 and 2 were isolated and identified as active principles. Compound 3 and 6 were isolated from the extracts of the flowers, and they showed significant inhibitory effects. 3. The structures of compounds 1, 2, 3, and 6 were identified as 4-hydroxycinnamic acid, 3,4-dihydroxy cinnamic acid methyl ester, 3-hydroxy-pyran-4-one, and (5-butyl-3-oxo-2,3-dihydro-furan-2-yl)-acetic acid, respectively. Compound 6 represents previously undescribed compound. 4. Compound 4 and 5 from the flowers have no inhibitory effects. It is interesting to note that these compounds did not show any activity despite the fact that two caffeic acid units are incorporated into the molecules. On the other hand, simple caffeic acid derivative such as compound 2 displayed a inhibitory effect. 5. The active compound 1, 2, 3, and 6 completely inhibited the germination of lettuce seeds at various concentrations. The IC_(50) concentrations of the active conpounds were at 816ppm, 945ppm, 1440ppm, and 421ppm, respectively. Benzoic acid was employed as a postive control. displaying IC_(50) concentrations 555ppm.
In the searching for the secondary metabolites with inhibitory effects on the germination of lettuce seeds, the methanol extracts from the leaves, stems, and flowers of Erigeron annuus were screened for their inhibitory effects, and the investigations of the active principles of the extracts were conducted by bioassay-guided fractionation. The summary of the study is as follows: 1. Methanol extracts from the leaves, stems, and flowers Erigeron annuus were concentrated, and suspended in water, and sequentially partitioned with n-hexane, EtOAc, and BuOH. Each fraction was tested for their activity, and EtOAc-soluble fractions from the leaves, stems, and flowers Erigeron annuus exhibited strong inhibitory effects at 5000 ppm level. 2. The above active EtOAc-soluble extracts were further fractionated on the basis of the bioassay results. From the extracts of the leaves and stems, compound 1 and 2 were isolated and identified as active principles. Compound 3 and 6 were isolated from the extracts of the flowers, and they showed significant inhibitory effects. 3. The structures of compounds 1, 2, 3, and 6 were identified as 4-hydroxycinnamic acid, 3,4-dihydroxy cinnamic acid methyl ester, 3-hydroxy-pyran-4-one, and (5-butyl-3-oxo-2,3-dihydro-furan-2-yl)-acetic acid, respectively. Compound 6 represents previously undescribed compound. 4. Compound 4 and 5 from the flowers have no inhibitory effects. It is interesting to note that these compounds did not show any activity despite the fact that two caffeic acid units are incorporated into the molecules. On the other hand, simple caffeic acid derivative such as compound 2 displayed a inhibitory effect. 5. The active compound 1, 2, 3, and 6 completely inhibited the germination of lettuce seeds at various concentrations. The IC_(50) concentrations of the active conpounds were at 816ppm, 945ppm, 1440ppm, and 421ppm, respectively. Benzoic acid was employed as a postive control. displaying IC_(50) concentrations 555ppm.
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