This study was carried out to assess the effect of superovulation response and quality of embryos recovered from cattle after a single subcutaneous injection of FSH dissolved in polyethylene glycol (PEG) in cattle. Cattle were allocated into control and 3 experimental treatment groups. In control, c...
This study was carried out to assess the effect of superovulation response and quality of embryos recovered from cattle after a single subcutaneous injection of FSH dissolved in polyethylene glycol (PEG) in cattle. Cattle were allocated into control and 3 experimental treatment groups. In control, cattle were injected intramuscularly 50 mg FSH twice daily for 4 d. Group 1 were injected subcutaneously with a single dose of 400 mg FSH dissolved in 30% PEG solution. Group 2 were injected subcutaneously with a single dose of 200 mg FSH dissolved in 30% PEG solution. Finally in group 3, cattle were injected twice 200 mg FSH dissolved in 30% PEG solution by subcutaneous. Superovulation was initiated by injection of FSH between Day 8 and 14 of the estrus cycle (Day 0, the day of estrus), and followed by injection of 25 mg PGF₂α at 48 hr after first FSH injection in order to facilitate the ovulation. Regardless whether behavioral estrus was detected, cows were then artificially inseminated (AI) with semen twice at 48 and 60 hr after PGF₂α injection. At 7 days after the second AI, ova/embryos were collected non-surgically by flushing the uterine horns with phosphate buffered saline supplemented with 2% fetal bovine serum. Embryos were counted and compared morphologically as being transferable and degenerated among different superovulation treatments. Furthermore, plasma concentrations of progesterone and estradiol-17β were measured by radioimmunoassay following different treatments at given days. All of cattle were observed in estrus in all treated groups, but 77.8% of cattle in control. Superovulation response was observed as 77.8, 87.5, 88.9, and 100% in control and Groups 1, 2 and 3. The mean numbers of corpus lutea (CL) detected in Group 1 were 19.6, which was significantly (P<0.05) higher than those in control, Group 2 and Group 3 (11.1, 13.4 and 9.6, respectively). However, there did not differ on the mean numbers of total ova/embryos recovered and of transferable embryos between control and treated groups. Higher rates of embryos classified as excellent were revealed in Group 3 than in others. In Group 2, only 4% of recovered ova remained in being unfertilized, whereas 29.7, 15.8 and 18.7% in control, Group 1 and Group 3, respectively. No difference in the pregnancy rate by transfer of recovered embryos was observed in control and treated groups (37.0 vs. 30-38%). Similar were the observations with the secretory patterns of blood progesterone and estradiol-17β in control and treated groups. In conclusion, a single subcutaneous injection with FSH dissolved in polyethylene glycol for the purpose of superovulation in cattle did not differ from multiple intramuscular injection protocol on the rates of recovery, transferable and pregnancy rate. However, a single injection is more practical for superovulation treatments than frequent injection because of reduction of stress in cattle and decreases of cost and labor.
This study was carried out to assess the effect of superovulation response and quality of embryos recovered from cattle after a single subcutaneous injection of FSH dissolved in polyethylene glycol (PEG) in cattle. Cattle were allocated into control and 3 experimental treatment groups. In control, cattle were injected intramuscularly 50 mg FSH twice daily for 4 d. Group 1 were injected subcutaneously with a single dose of 400 mg FSH dissolved in 30% PEG solution. Group 2 were injected subcutaneously with a single dose of 200 mg FSH dissolved in 30% PEG solution. Finally in group 3, cattle were injected twice 200 mg FSH dissolved in 30% PEG solution by subcutaneous. Superovulation was initiated by injection of FSH between Day 8 and 14 of the estrus cycle (Day 0, the day of estrus), and followed by injection of 25 mg PGF₂α at 48 hr after first FSH injection in order to facilitate the ovulation. Regardless whether behavioral estrus was detected, cows were then artificially inseminated (AI) with semen twice at 48 and 60 hr after PGF₂α injection. At 7 days after the second AI, ova/embryos were collected non-surgically by flushing the uterine horns with phosphate buffered saline supplemented with 2% fetal bovine serum. Embryos were counted and compared morphologically as being transferable and degenerated among different superovulation treatments. Furthermore, plasma concentrations of progesterone and estradiol-17β were measured by radioimmunoassay following different treatments at given days. All of cattle were observed in estrus in all treated groups, but 77.8% of cattle in control. Superovulation response was observed as 77.8, 87.5, 88.9, and 100% in control and Groups 1, 2 and 3. The mean numbers of corpus lutea (CL) detected in Group 1 were 19.6, which was significantly (P<0.05) higher than those in control, Group 2 and Group 3 (11.1, 13.4 and 9.6, respectively). However, there did not differ on the mean numbers of total ova/embryos recovered and of transferable embryos between control and treated groups. Higher rates of embryos classified as excellent were revealed in Group 3 than in others. In Group 2, only 4% of recovered ova remained in being unfertilized, whereas 29.7, 15.8 and 18.7% in control, Group 1 and Group 3, respectively. No difference in the pregnancy rate by transfer of recovered embryos was observed in control and treated groups (37.0 vs. 30-38%). Similar were the observations with the secretory patterns of blood progesterone and estradiol-17β in control and treated groups. In conclusion, a single subcutaneous injection with FSH dissolved in polyethylene glycol for the purpose of superovulation in cattle did not differ from multiple intramuscular injection protocol on the rates of recovery, transferable and pregnancy rate. However, a single injection is more practical for superovulation treatments than frequent injection because of reduction of stress in cattle and decreases of cost and labor.
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