식물체내에서 paraquat 살초작용 과정 중에 독성산소를 발생시킨다. 이러한 독성산소에는 활성산소종인 singlet oxygen, hydroxyl radical, superoxide radical, hydrogen peroxide 등이 있다. 본 연구에서는 ...
식물체내에서 paraquat 살초작용 과정 중에 독성산소를 발생시킨다. 이러한 독성산소에는 활성산소종인 singlet oxygen, hydroxyl radical, superoxide radical, hydrogen peroxide 등이 있다. 본 연구에서는 지황에서 추출한 acteoside의 항산화 효과를 천연항산화제 3종 (α-tocoperol, ascorbic acid, resveratol) 및 합성항산화제 2종(butylated hydroxyanisole, 3,5-di-tert-butyl-4-hydroxy-toluene)과 비교 조사하였다. Singlet oxygen에 대한 소거능력은 acteoside가 0 ~ 0.1mM농도에서 급격한 소거 효과를 나타냈고, 0.1 mM 이상 농도부터는 포화상태를 유지하였다. Hydroxyl radical 소거능력에 대해서 acteoside는 ascorbic acid에 비하여 0.2mM에서 2.0배, 1mM에서는 3.5배로 acteoside의 항산화 효과가 훨씬 높았다. Superoxide anion 소거는 acteoside가 0.5 mM에서 58.5%, 1 mM에서 96.9%의 효과를 나타내었다. Hydrogen peroxide의 소거는 acteoside가 2 mM에서 22.5%로 높지 않았지만 2 ~ 5 mM까지는 급격하게 증가하여 5mM에서 100%의 소거 효과를 나타내었다. 활성산소종 소거효과에 대해 전체적인 능력을 비교한 결과 acteoside가 가장 강한 소거능력을 나타내었다. 한편 paraquat 0.4mM과 각각의 항산화제 0.2, 1 및 5mM을 혼합하여 콩, 밀, 옥수수, 목화 및 벼의 잎에 경엽처리 하여 paraquat의 활성억제 효과를 in vivo로 검토한 결과, acteoside와 resveratrol이 가장 높은 저해 능력을 나타내었다. 이상의 연구결과는 지황내 acteoside가 paraquat를 처리 하였을 때 발생하는 독성산소의 생성을 억제하거나 또는 발생된 독성산소를 소거하여 paraquat를 불활성화 시키고 무독화시킴에 따라 paraquat에 대한 지황 내성을 부여하는 것으로 생각되었다.
식물체내에서 paraquat 살초작용 과정 중에 독성산소를 발생시킨다. 이러한 독성산소에는 활성산소종인 singlet oxygen, hydroxyl radical, superoxide radical, hydrogen peroxide 등이 있다. 본 연구에서는 지황에서 추출한 acteoside의 항산화 효과를 천연항산화제 3종 (α-tocoperol, ascorbic acid, resveratol) 및 합성항산화제 2종(butylated hydroxyanisole, 3,5-di-tert-butyl-4-hydroxy-toluene)과 비교 조사하였다. Singlet oxygen에 대한 소거능력은 acteoside가 0 ~ 0.1mM농도에서 급격한 소거 효과를 나타냈고, 0.1 mM 이상 농도부터는 포화상태를 유지하였다. Hydroxyl radical 소거능력에 대해서 acteoside는 ascorbic acid에 비하여 0.2mM에서 2.0배, 1mM에서는 3.5배로 acteoside의 항산화 효과가 훨씬 높았다. Superoxide anion 소거는 acteoside가 0.5 mM에서 58.5%, 1 mM에서 96.9%의 효과를 나타내었다. Hydrogen peroxide의 소거는 acteoside가 2 mM에서 22.5%로 높지 않았지만 2 ~ 5 mM까지는 급격하게 증가하여 5mM에서 100%의 소거 효과를 나타내었다. 활성산소종 소거효과에 대해 전체적인 능력을 비교한 결과 acteoside가 가장 강한 소거능력을 나타내었다. 한편 paraquat 0.4mM과 각각의 항산화제 0.2, 1 및 5mM을 혼합하여 콩, 밀, 옥수수, 목화 및 벼의 잎에 경엽처리 하여 paraquat의 활성억제 효과를 in vivo로 검토한 결과, acteoside와 resveratrol이 가장 높은 저해 능력을 나타내었다. 이상의 연구결과는 지황내 acteoside가 paraquat를 처리 하였을 때 발생하는 독성산소의 생성을 억제하거나 또는 발생된 독성산소를 소거하여 paraquat를 불활성화 시키고 무독화시킴에 따라 paraquat에 대한 지황 내성을 부여하는 것으로 생각되었다.
Active oxygen species (AOS; singlet oxygen, hydroxyl radical, superoxide radical, and hydrogen peroxide) are generated during the mode of action of paraquat in plants. In this research, AOS scavenging effects of a specific antioxidant, acteoside, extracted from Rehmannia glutinosa (Chinese foxglove)...
Active oxygen species (AOS; singlet oxygen, hydroxyl radical, superoxide radical, and hydrogen peroxide) are generated during the mode of action of paraquat in plants. In this research, AOS scavenging effects of a specific antioxidant, acteoside, extracted from Rehmannia glutinosa (Chinese foxglove) were investigated as comparing with those of natural antioxidents (α-tocopherol, ascorbic acid, and resveratrol) and synthetic antioxidants (butylated hydroxyanisole, BHA and 3,5-di-tert-butyl-4-hydroxy-toluene, BHT). Singlet oxygen was clearly scavenged by 0 to 0.1 mM of acteoside, whereas the scavenging effect was not much improved with more than 0.1 mM of acteoside application. Scavenging effect of acteoside on hydroxyl radical was 2.0 times higher than those of ascorbic acid applied at 0.2 mM, and then it was 3.5 times higher at 1.0 mM application of the antioxidant. Treatment of 0.5 mM acteoside scavenged only 58.5% of superoxide anions, but the scavenging effect on superoxide anions was drastically increased until 96.9% with 1.0 mM acteoside application. Also, the hydrogen peroxide was scavenged only 22.5% by the treatment of 2.0 mM acteoside, but complete scavenging effect was obtained with 5.0 mM of acteoside. With in vivo experiments, inhibition on paraquat activity was the highest with the applications of acteoside and resveratrol when 0.4 mM paraquat with various concentrations (0, 0.2, 1.0, and 5.0 mM) of each antioxidant was foliage-applied to different plant species such as soybean, wheat, corn, and cotton, and rice. These results indicate that the specific antioxidant, acteoside, in Chinese foxglove inhibits producing AOS or scavenges AOS generated during paraquat action. Thus, paraquat tolerance of Chinese foxglove might partly be attributed to either paraquat inactivation or scavenging activity by acteoside or both in the plant.
Active oxygen species (AOS; singlet oxygen, hydroxyl radical, superoxide radical, and hydrogen peroxide) are generated during the mode of action of paraquat in plants. In this research, AOS scavenging effects of a specific antioxidant, acteoside, extracted from Rehmannia glutinosa (Chinese foxglove) were investigated as comparing with those of natural antioxidents (α-tocopherol, ascorbic acid, and resveratrol) and synthetic antioxidants (butylated hydroxyanisole, BHA and 3,5-di-tert-butyl-4-hydroxy-toluene, BHT). Singlet oxygen was clearly scavenged by 0 to 0.1 mM of acteoside, whereas the scavenging effect was not much improved with more than 0.1 mM of acteoside application. Scavenging effect of acteoside on hydroxyl radical was 2.0 times higher than those of ascorbic acid applied at 0.2 mM, and then it was 3.5 times higher at 1.0 mM application of the antioxidant. Treatment of 0.5 mM acteoside scavenged only 58.5% of superoxide anions, but the scavenging effect on superoxide anions was drastically increased until 96.9% with 1.0 mM acteoside application. Also, the hydrogen peroxide was scavenged only 22.5% by the treatment of 2.0 mM acteoside, but complete scavenging effect was obtained with 5.0 mM of acteoside. With in vivo experiments, inhibition on paraquat activity was the highest with the applications of acteoside and resveratrol when 0.4 mM paraquat with various concentrations (0, 0.2, 1.0, and 5.0 mM) of each antioxidant was foliage-applied to different plant species such as soybean, wheat, corn, and cotton, and rice. These results indicate that the specific antioxidant, acteoside, in Chinese foxglove inhibits producing AOS or scavenges AOS generated during paraquat action. Thus, paraquat tolerance of Chinese foxglove might partly be attributed to either paraquat inactivation or scavenging activity by acteoside or both in the plant.
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