Abstract ▼ AI-Helper

The free radical scavenging activities and the protective effects against oxidative damage induced by the reactive oxygen species(ROS) of Rhus javanica extracts were investigated. n-Hexane, ethylacetate and water fractions were prepared from methanol extract. DPPH radical and superoxide anion scaven...

The free radical scavenging activities and the protective effects against oxidative damage induced by the reactive oxygen species(ROS) of Rhus javanica extracts were investigated. n-Hexane, ethylacetate and water fractions were prepared from methanol extract. DPPH radical and superoxide anion scavenging activities were estimated. The inhibitory activities of lipid peroxidation were measured by the thiocyanate method and by the amount of tissue TBARS production in the mouse liver and brain homogenate. Intracellular ROS formation was quantified using fluorescent probes, 2',7'-dichlorofluorescin diacetate (DCFH-DA) and dihydroethidium(DHE). The oxidative DNA damage was investigated by the Comet assay in HepG_(2) cells exposed either to H_(2)O_(2) or to menadione. The highest IC_(50) values both in the DPPH radical and in the superoxide anion scavenging activities were found in the ethylacetate fraction with the values of 5.38 ㎍/㎖ and 0.16 ㎍/㎖, respectively. Ethylacetate fraction also showed the greatest protection against lipid peroxidation both in the linoleic acid autooxidation and in the tissue TBARS generation. Especially, the antioxidative effect against linoleic acid autooxidation of ethylacetate fraction is as strong as BHT, which is representative synthetic antioxidant. Intracellular ROS formation and DNA damage in HepG_(2) cells pre-treated with the various concentrations of Rhus javanica ethylacetate extracts and then incubated either with H_(2)O_(2) or with menadione were inhibited in a dose-dependent manner. These findings demonstrate that Rhus javanica might have biologically active components which have strong protective effects against ROS induced oxidative damages to the biomolecules, such as cell membranes and DNA, which might be the cause of various kinds of diseases and aging. Therefore, we suggest that further studies for the identification and the mechanisms of action of these components would be necessary.

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