Mushrooms have been considered as a health food so that their pharmacological effects have been extensively studied. It is known that sonle polysaccharides from higher plants, fungi, yeast, and bacterial have anticancer activity. The anhcancer activity of those polysaccharides, especially from basid...
Mushrooms have been considered as a health food so that their pharmacological effects have been extensively studied. It is known that sonle polysaccharides from higher plants, fungi, yeast, and bacterial have anticancer activity. The anhcancer activity of those polysaccharides, especially from basidiomycetes including Ganoderma, Phellinus, and Agaricales, have been the focus of the investigation. However, investigation on the anivial activity and immuno activity with those basidiornycestes is in initial stage. In order to study on antiviral activity of extracts from two basidiomycetes, two water extracts and three solvent extracts of Phellinus baumii (PB) and Ganoderma lucidum (GL), respectively, were prepared from carpophores of the two mushrooms. The five kinds of extracts was tested for their cytotoxicities using MTT assay. PB hot water extract was toxic with l0mg/ml but not toxic below 10 mg/ml. PB methanol and ethyl acetate extracts were toxic between 1 mg/ml and 1 μg/ml but was not toxic below 1μg/m1. However, hexane extract was toxic at all studied concentrations. GL hot water extract was toxic between l0 mg/ml and l0 μg/ml but not toxic below 10 pg/ml. GL methanol, hexane, and ethyl acetate extracts were toxic at l mg/nl but was not toxic below 10 pg/ml. Although extract concentrates of the two mushrooms had some celIular toxicity,normal extract had no cellular toxicity. Those extracts were examied for their activities against influenza A virus(Flu A) in vitro.Antiviral achvities were evaluated by the cytopathic effect(CPE) inhibition assay. PB water and ethyl acetate extract had anhviral activity. Especially, the ethyl acetate extract had significant inhibition on cytopathic effects. GL water extract also had antiviral activity but the activity was lower than those of PB extracts. When immuno activity was assessed by colorimetic method using XTT, PB and GL cold water extracts showed strong lympoblastogenic activity on mouse splenic lymphocytes in that it stimulated the cells to proliferate. When immuno activity Was measured using now cytometrical analyses, GL cold water extract showed an increase of cell volume, indicating an initial lymphoblastogenic effect. When immuno activity was measured using direct immunofluorescence staining with flow cytometrical anaIyses where monoclonl antibodies for CD4 (helper T cel1), CD8 (cytotoxic T cell) were used, the raho of CD4 cells were increased in the presence of various extracts including PB hot water and aIl three solvent extracts and GL hot water, hexane, and methanol extracts. CD8 cells were increased in the presence of GL hot water extracts. All the above results indicate the possibility of development of antiviral agents frorn basidiomycetous fungi.
Mushrooms have been considered as a health food so that their pharmacological effects have been extensively studied. It is known that sonle polysaccharides from higher plants, fungi, yeast, and bacterial have anticancer activity. The anhcancer activity of those polysaccharides, especially from basidiomycetes including Ganoderma, Phellinus, and Agaricales, have been the focus of the investigation. However, investigation on the anivial activity and immuno activity with those basidiornycestes is in initial stage. In order to study on antiviral activity of extracts from two basidiomycetes, two water extracts and three solvent extracts of Phellinus baumii (PB) and Ganoderma lucidum (GL), respectively, were prepared from carpophores of the two mushrooms. The five kinds of extracts was tested for their cytotoxicities using MTT assay. PB hot water extract was toxic with l0mg/ml but not toxic below 10 mg/ml. PB methanol and ethyl acetate extracts were toxic between 1 mg/ml and 1 μg/ml but was not toxic below 1μg/m1. However, hexane extract was toxic at all studied concentrations. GL hot water extract was toxic between l0 mg/ml and l0 μg/ml but not toxic below 10 pg/ml. GL methanol, hexane, and ethyl acetate extracts were toxic at l mg/nl but was not toxic below 10 pg/ml. Although extract concentrates of the two mushrooms had some celIular toxicity,normal extract had no cellular toxicity. Those extracts were examied for their activities against influenza A virus(Flu A) in vitro.Antiviral achvities were evaluated by the cytopathic effect(CPE) inhibition assay. PB water and ethyl acetate extract had anhviral activity. Especially, the ethyl acetate extract had significant inhibition on cytopathic effects. GL water extract also had antiviral activity but the activity was lower than those of PB extracts. When immuno activity was assessed by colorimetic method using XTT, PB and GL cold water extracts showed strong lympoblastogenic activity on mouse splenic lymphocytes in that it stimulated the cells to proliferate. When immuno activity Was measured using now cytometrical analyses, GL cold water extract showed an increase of cell volume, indicating an initial lymphoblastogenic effect. When immuno activity was measured using direct immunofluorescence staining with flow cytometrical anaIyses where monoclonl antibodies for CD4 (helper T cel1), CD8 (cytotoxic T cell) were used, the raho of CD4 cells were increased in the presence of various extracts including PB hot water and aIl three solvent extracts and GL hot water, hexane, and methanol extracts. CD8 cells were increased in the presence of GL hot water extracts. All the above results indicate the possibility of development of antiviral agents frorn basidiomycetous fungi.
주제어
#상황버섯 영지버섯 항바이러스 면역활성능
※ AI-Helper는 부적절한 답변을 할 수 있습니다.