Present experiments were carried out to examine the methods for in vitro masspropagation of four carnivorous species with potential for industrial use Darlingtonia californica, Heliamphora minor, Sarracenia purpurea and Pinguicula moranensis. Also studies on optimum soil mix and watering for th seed...
Present experiments were carried out to examine the methods for in vitro masspropagation of four carnivorous species with potential for industrial use Darlingtonia californica, Heliamphora minor, Sarracenia purpurea and Pinguicula moranensis. Also studies on optimum soil mix and watering for th seedling removed from the culture vessels were performed. In D. californica, H. minor and S. purpurea young plantlets were good as explant source and P. moranensis whole leaves. Shoot multiplication and growth was promoted by 2 mg·L-1 BA in D. californica, 1 mg·L-1 BA + 2 mg·L-1 NAA in H. minor, 1 mg·L-1 kinetin in S. purpurea and 1 mg·L-1 kinetin + 1 mg·L-1 IAA in P. moranensis. Four type of media(1/4, 1/2, 1MS and Parliman) were employed and total nitrogen contents were 15, 30, and 60 mM. The ratio of NH4+:NO3- as nitrogen source were 30:0~0:30 mM. Sucrose contents changed from 0~5%, agar 0 to 10%, activated charcoal 0 to 2%, pH from 4.0~6.0%, temperature 15 to 30℃, and culturing was alone under light illumination after 0~4 weeks of darkness. The test culturing conditions for D. californica were 1/2MS media, 15 mM * A thesis for the Degree of Master on February 2007.of total nitrogen contents, 2~3% sucrose, no agar and light illumination after 1~2 weeks of darkness. For H. minor, they were 1MS media, 30 mM total nitrogen contents, pH 5.5, and no agar, the multiplication of shoot were contained by 1~2% sucrose while root multiplication by 3~5% sucrose. In S. purpurea, shoot multiplication and growth were promoted by Parliman media, and high total nitrogen contents increased shoot multiplication but, inhibited root development. High sucrose contents increased shoot multiplication, and activated charcoal generally promoted multiplication and development of shoot and roots. In P. moranensis, test growth were obtained by 1/2MS, 60 mM of total nitrogen contents, 5% sucrose and 0.8% agar. Acclimatization studies were performed using different soil mixes and watering methods. Eight mixes were used, employing different combinations of horticultural mixes, peatmoss, perlite and sands. In D. californica, H. minor, and P. moranensis plant survival rate and growth were highest with only peatmoss or peatmoss and perlite mix, but in S. purpurea with only peatmoss. Various kinds of peatmoss were employed in this experiment and in D. californica test growth was obtained with horticultural compost or Acadian peatmoss. In H. minor and S. purpurea, it was obtained with Sunshine peatmoss + perlite mixes. Two types of watering were performed surface or subsurface. In D. californica surface watering resulted in highest rate, and 2 subsurface watering for 15 minutes each showed good survival rate. Plant growth and multiplication were promoted by 2 subsurface watering for 48 minutes each in H. minor. To evaluate effects IBA on rooting in vitro, different concentration soaking from 0 to 10 mg·L-1 were added to media. In D. californica, no IBA resulted in good shoot multiplication and growth, and good root formation. In H. minor, 2, 5 mg·L-1 showed best growth response. Young plantlets were soaked with different concentration of IBA for different periods of treatments, and cultured in vitro. In D. californica, low IBA concentration with low treatment periods rusulted in best growth and rooting. However, in H. minor high concentration with longer periods rusulted in best growth and rooting. In H. minor young plantlets from culture vessels were soaked with IBA and grown on soil mixes. Shoot soaking periods resulted in good growth regulation of concentration with 200 mg·L-1 for 3 hours being best treatment.
Present experiments were carried out to examine the methods for in vitro masspropagation of four carnivorous species with potential for industrial use Darlingtonia californica, Heliamphora minor, Sarracenia purpurea and Pinguicula moranensis. Also studies on optimum soil mix and watering for th seedling removed from the culture vessels were performed. In D. californica, H. minor and S. purpurea young plantlets were good as explant source and P. moranensis whole leaves. Shoot multiplication and growth was promoted by 2 mg·L-1 BA in D. californica, 1 mg·L-1 BA + 2 mg·L-1 NAA in H. minor, 1 mg·L-1 kinetin in S. purpurea and 1 mg·L-1 kinetin + 1 mg·L-1 IAA in P. moranensis. Four type of media(1/4, 1/2, 1MS and Parliman) were employed and total nitrogen contents were 15, 30, and 60 mM. The ratio of NH4+:NO3- as nitrogen source were 30:0~0:30 mM. Sucrose contents changed from 0~5%, agar 0 to 10%, activated charcoal 0 to 2%, pH from 4.0~6.0%, temperature 15 to 30℃, and culturing was alone under light illumination after 0~4 weeks of darkness. The test culturing conditions for D. californica were 1/2MS media, 15 mM * A thesis for the Degree of Master on February 2007.of total nitrogen contents, 2~3% sucrose, no agar and light illumination after 1~2 weeks of darkness. For H. minor, they were 1MS media, 30 mM total nitrogen contents, pH 5.5, and no agar, the multiplication of shoot were contained by 1~2% sucrose while root multiplication by 3~5% sucrose. In S. purpurea, shoot multiplication and growth were promoted by Parliman media, and high total nitrogen contents increased shoot multiplication but, inhibited root development. High sucrose contents increased shoot multiplication, and activated charcoal generally promoted multiplication and development of shoot and roots. In P. moranensis, test growth were obtained by 1/2MS, 60 mM of total nitrogen contents, 5% sucrose and 0.8% agar. Acclimatization studies were performed using different soil mixes and watering methods. Eight mixes were used, employing different combinations of horticultural mixes, peatmoss, perlite and sands. In D. californica, H. minor, and P. moranensis plant survival rate and growth were highest with only peatmoss or peatmoss and perlite mix, but in S. purpurea with only peatmoss. Various kinds of peatmoss were employed in this experiment and in D. californica test growth was obtained with horticultural compost or Acadian peatmoss. In H. minor and S. purpurea, it was obtained with Sunshine peatmoss + perlite mixes. Two types of watering were performed surface or subsurface. In D. californica surface watering resulted in highest rate, and 2 subsurface watering for 15 minutes each showed good survival rate. Plant growth and multiplication were promoted by 2 subsurface watering for 48 minutes each in H. minor. To evaluate effects IBA on rooting in vitro, different concentration soaking from 0 to 10 mg·L-1 were added to media. In D. californica, no IBA resulted in good shoot multiplication and growth, and good root formation. In H. minor, 2, 5 mg·L-1 showed best growth response. Young plantlets were soaked with different concentration of IBA for different periods of treatments, and cultured in vitro. In D. californica, low IBA concentration with low treatment periods rusulted in best growth and rooting. However, in H. minor high concentration with longer periods rusulted in best growth and rooting. In H. minor young plantlets from culture vessels were soaked with IBA and grown on soil mixes. Shoot soaking periods resulted in good growth regulation of concentration with 200 mg·L-1 for 3 hours being best treatment.
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