A total process for red ginseng polysaccharide (RGPS), a potential immunostimulatory and anticancer component, was proposed. The experimental conditions for extraction and recovery process were optimized and statistically analyzed to obtain an efficient and economical process. The process feasibilit...
A total process for red ginseng polysaccharide (RGPS), a potential immunostimulatory and anticancer component, was proposed. The experimental conditions for extraction and recovery process were optimized and statistically analyzed to obtain an efficient and economical process. The process feasibility was verified on a pilot-scale. Additionally a formulated food product, chewable tablet containing various foodstuffs, was developed using the RGPS as a key ingredient. First, the chemical compositions of various raw materials used for extraction process, such as white ginseng (WG), red ginseng (RG) and red ginseng marc (RGM), were analyzed and compared. The contents of protein, lipid and ash of RGM were very similar to those of WG and RG. However, the crude fiber content of RGM (15.3%), was greatly higher than that of WG (2.1%) and RG (0.5%). The contents of crude polysaccharide of RGM (19.8%) were similar to those of RG (23.3%), root hair of ginseng (RHG) (21.8) and black ginseng (BG) (15.6%). Even thougth RGM is a byproduct of saponin manufacturing process and has been considered as a waste from red ginseng extraction process, RGM was suggested as a cost-effective raw material for producing polysaccharide in the present study, on the basis of the chemical composition analysis result. Second, the polysaccharide extraction and recovery conditions such as pH, temperature, time and alcohol addition ratio were optimized. From the experimental work and stastical analysis, it was recommended to extract the polysaccharide from RGM by hot water (pH 6.5~7.0) at 85℃ for 4 hours. And the recovery yield was the highest when the volume ratio of extract and ethyl alcohol was 1 : 5. Third, the recovery process using alcohol precipitation was replaced by a novel process using a ultrafiltration (UF) process for a large-scale manufacturing, and the process conditions were established. On a lab-scale UF system in which plate and frame type membrane is used, the polysaccharide yields was the highest when a membrane of MWCO 10K was used. The recovery yields were determined based on various parameters such as total saccharide, crude polysaccharide, acidic polysaccharide and dry matter, as 78.8%, 60.8%, 36.2%, 86.5%, respectively. The sugar compositions of the isolated polysaccharide were identified by HPLC as glucose : galactose : arabinose = 91.1 : 4.4 : 4.5. And the polysaccharide consist of sugars more than 51.6%, and the average molecular weight was lower than 40 kDa. The MWCO of UF membrane was selected as 20,000 on a pilot-scale UF system using a tubular type membrane module. Fourth, the biological activities of polysaccharide such as anticancer activity was investigated in vitro by CCK assay using a human lung cancer cell line (A-549) and a human lung normal cell line (L-132). The cancer cell inhibition rate of red ginseng polysaccharide on A-549 was slightly lower than that of indole-3-carbinol (I3C), which is a positive control in the assay. However, the toxicity of the red ginseng polysaccharide on L-132 was lower than that of I3C. Finally, a formulated functional product using the RGPS as a key component was developed. The dosage form was selected as chewable tablet. In addition to RGPS, various main ingredients such as soy lecithin, γ-aminobutyric acid (GABA), selenium and taurine, diluents, lubricants, binders and sweetener for chewable tablet were used. The formulation was established through sensory evaluations and quality controls. In conclusion, the potential application model using RGPS was proposed in the present study and the detail manufacturing conditions including extraction conditions, membrane process conditions and the press conditions for a tablet product was established for industrial application.
A total process for red ginseng polysaccharide (RGPS), a potential immunostimulatory and anticancer component, was proposed. The experimental conditions for extraction and recovery process were optimized and statistically analyzed to obtain an efficient and economical process. The process feasibility was verified on a pilot-scale. Additionally a formulated food product, chewable tablet containing various foodstuffs, was developed using the RGPS as a key ingredient. First, the chemical compositions of various raw materials used for extraction process, such as white ginseng (WG), red ginseng (RG) and red ginseng marc (RGM), were analyzed and compared. The contents of protein, lipid and ash of RGM were very similar to those of WG and RG. However, the crude fiber content of RGM (15.3%), was greatly higher than that of WG (2.1%) and RG (0.5%). The contents of crude polysaccharide of RGM (19.8%) were similar to those of RG (23.3%), root hair of ginseng (RHG) (21.8) and black ginseng (BG) (15.6%). Even thougth RGM is a byproduct of saponin manufacturing process and has been considered as a waste from red ginseng extraction process, RGM was suggested as a cost-effective raw material for producing polysaccharide in the present study, on the basis of the chemical composition analysis result. Second, the polysaccharide extraction and recovery conditions such as pH, temperature, time and alcohol addition ratio were optimized. From the experimental work and stastical analysis, it was recommended to extract the polysaccharide from RGM by hot water (pH 6.5~7.0) at 85℃ for 4 hours. And the recovery yield was the highest when the volume ratio of extract and ethyl alcohol was 1 : 5. Third, the recovery process using alcohol precipitation was replaced by a novel process using a ultrafiltration (UF) process for a large-scale manufacturing, and the process conditions were established. On a lab-scale UF system in which plate and frame type membrane is used, the polysaccharide yields was the highest when a membrane of MWCO 10K was used. The recovery yields were determined based on various parameters such as total saccharide, crude polysaccharide, acidic polysaccharide and dry matter, as 78.8%, 60.8%, 36.2%, 86.5%, respectively. The sugar compositions of the isolated polysaccharide were identified by HPLC as glucose : galactose : arabinose = 91.1 : 4.4 : 4.5. And the polysaccharide consist of sugars more than 51.6%, and the average molecular weight was lower than 40 kDa. The MWCO of UF membrane was selected as 20,000 on a pilot-scale UF system using a tubular type membrane module. Fourth, the biological activities of polysaccharide such as anticancer activity was investigated in vitro by CCK assay using a human lung cancer cell line (A-549) and a human lung normal cell line (L-132). The cancer cell inhibition rate of red ginseng polysaccharide on A-549 was slightly lower than that of indole-3-carbinol (I3C), which is a positive control in the assay. However, the toxicity of the red ginseng polysaccharide on L-132 was lower than that of I3C. Finally, a formulated functional product using the RGPS as a key component was developed. The dosage form was selected as chewable tablet. In addition to RGPS, various main ingredients such as soy lecithin, γ-aminobutyric acid (GABA), selenium and taurine, diluents, lubricants, binders and sweetener for chewable tablet were used. The formulation was established through sensory evaluations and quality controls. In conclusion, the potential application model using RGPS was proposed in the present study and the detail manufacturing conditions including extraction conditions, membrane process conditions and the press conditions for a tablet product was established for industrial application.
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#홍삼 다당체 건강기능식품 Red Ginseng Polysaccharide
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