Abundant phenolic compounds such as phenolic acids, flavonoids were found in Chinese quince, in quantity about 4 times higher than that found in other quince fruit and 20 times higher than that found in apple. Because of that, it possesses high antioxidant ability, anti-inflammatory capacity, antial...
Abundant phenolic compounds such as phenolic acids, flavonoids were found in Chinese quince, in quantity about 4 times higher than that found in other quince fruit and 20 times higher than that found in apple. Because of that, it possesses high antioxidant ability, anti-inflammatory capacity, antiallergic effects, reducing the risk of coronary heart diseases and cancer. In the present study , primary components contained in Chinese quince were analyzed according to AOAC method firstly . The primary components contained in dried Chinese quince powder were moisture of 10.16%, ash of 13.96%, crude fiber of 11.03%, crude fat of 3.42%, vitamin C of 61.1 mg/100 g , and crude protein of 2.50%. Secondly, the main research focus on the extraction of phenolic com pounds from Chinese quince by microwave-assisted extraction (MAE) and the optimization of process parameters aiming at maximal phenolic compounds extraction . The MAE experiments were carried out in accordance with a central composite design (CCD) which containing five levels and three variables, leading to 20 runs in total. The best combinations of process variables such as ethanol concentration, extraction time and microwave power were obtained for the maximum extraction of total phenolics content (TPC) , total flavonoids content (TFC), electron donating ability (EDA) and total soluble solid yield (TSSY) using response surface methodology (RSM). According to analysis of variance (ANOVA), t he ethanol concentration and microwave power have a great significant effect (P<0.001) on microwave-assisted extraction process. The optimal microwave-assisted extraction conditions obtained by superimposed contour plots were as follows: extraction time of 5 min, ethanol concentration of 45.05%, and microwave power of 110.8 W, which could lead to maximal total flavon o ids content of 480.1 mg RE/100 g, total phenolics content of 2232 mg GAE/100 g, electron donating ability of 77.46% and total soluble solid yield of 43.6% in Chinese quince extract. V alidation experiment was carried out at above mentioned optimal extraction conditions, and the results of each response were the total flavon o ids content ( 472.277 ± 12.014 mg RE/100 g ), total phenolics content ( 2269 ± 77.92 GAE/100 g ) , electr on donating ability ( 74.37 ± 1.33%), and total soluble solid yield (44.06 ± 0.91%), which were well matched the predicted value and confirmed the validity of this research. Thirdly, exact categories and quantity of phenolic acids contained in Chinese quince were obtained through HPLC analysis. HPLC analysis results showed that five kinds of phenolic acids have been detected in Chinese quince extract . The quantities of these five kinds of phenolic acids were 0.0023 mg/mL gallic acid , 0.0543 mg/mL p-hydrobenzoic acid, 0.1732 mg/mL protocatechic acid, 0.0043 mg/ mL p-courmaric acid, and 0.0235 mg/ mL ferulic acid . Based on all the results obtained in the present study, by compar ing to other reports on other extraction means to extract phenolic components from Chinese quince, MAE is more efficient, and it can extract substantial quantity of phenolic compounds in a short period .
Abundant phenolic compounds such as phenolic acids, flavonoids were found in Chinese quince, in quantity about 4 times higher than that found in other quince fruit and 20 times higher than that found in apple. Because of that, it possesses high antioxidant ability, anti-inflammatory capacity, antiallergic effects, reducing the risk of coronary heart diseases and cancer. In the present study , primary components contained in Chinese quince were analyzed according to AOAC method firstly . The primary components contained in dried Chinese quince powder were moisture of 10.16%, ash of 13.96%, crude fiber of 11.03%, crude fat of 3.42%, vitamin C of 61.1 mg/100 g , and crude protein of 2.50%. Secondly, the main research focus on the extraction of phenolic com pounds from Chinese quince by microwave-assisted extraction (MAE) and the optimization of process parameters aiming at maximal phenolic compounds extraction . The MAE experiments were carried out in accordance with a central composite design (CCD) which containing five levels and three variables, leading to 20 runs in total. The best combinations of process variables such as ethanol concentration, extraction time and microwave power were obtained for the maximum extraction of total phenolics content (TPC) , total flavonoids content (TFC), electron donating ability (EDA) and total soluble solid yield (TSSY) using response surface methodology (RSM). According to analysis of variance (ANOVA), t he ethanol concentration and microwave power have a great significant effect (P<0.001) on microwave-assisted extraction process. The optimal microwave-assisted extraction conditions obtained by superimposed contour plots were as follows: extraction time of 5 min, ethanol concentration of 45.05%, and microwave power of 110.8 W, which could lead to maximal total flavon o ids content of 480.1 mg RE/100 g, total phenolics content of 2232 mg GAE/100 g, electron donating ability of 77.46% and total soluble solid yield of 43.6% in Chinese quince extract. V alidation experiment was carried out at above mentioned optimal extraction conditions, and the results of each response were the total flavon o ids content ( 472.277 ± 12.014 mg RE/100 g ), total phenolics content ( 2269 ± 77.92 GAE/100 g ) , electr on donating ability ( 74.37 ± 1.33%), and total soluble solid yield (44.06 ± 0.91%), which were well matched the predicted value and confirmed the validity of this research. Thirdly, exact categories and quantity of phenolic acids contained in Chinese quince were obtained through HPLC analysis. HPLC analysis results showed that five kinds of phenolic acids have been detected in Chinese quince extract . The quantities of these five kinds of phenolic acids were 0.0023 mg/mL gallic acid , 0.0543 mg/mL p-hydrobenzoic acid, 0.1732 mg/mL protocatechic acid, 0.0043 mg/ mL p-courmaric acid, and 0.0235 mg/ mL ferulic acid . Based on all the results obtained in the present study, by compar ing to other reports on other extraction means to extract phenolic components from Chinese quince, MAE is more efficient, and it can extract substantial quantity of phenolic compounds in a short period .
주제어
#optimization response surface methodology microwave-assisted extraction phenolic compound Chinese quince
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