Fruit peels of Citrus unshiu Marc., Citrus erythrosa Hort, Citrus sunki Hort, Citrus benlkoji Hort, Citrus avrantium L., Citrus leiocarpa Hort. et Tanaka, Citrus playmamma Hort, Citrus nippokoreana and Citrus natsudaidai Hayata were analyzed for flavonoids via high performance liquid chromatography ...
Fruit peels of Citrus unshiu Marc., Citrus erythrosa Hort, Citrus sunki Hort, Citrus benlkoji Hort, Citrus avrantium L., Citrus leiocarpa Hort. et Tanaka, Citrus playmamma Hort, Citrus nippokoreana and Citrus natsudaidai Hayata were analyzed for flavonoids via high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) in the positive mode with selected ion monitoring (SIM). Among fortynine flavonoid components labeled, five flavanones, twenty tow flavones and tow coumarins were identified for the first time; naringin (3), poncirin (9), trihydroxytetramethoxyflavone-glucoside (11), meranzin (15), hexamethoxyflavone isomers (12, 25, 28 and 45), hydroxypentamethoxyflavone (16, 22), pentahydroxymethoxyflavone (17), isomeranzin (18), isosinensetin (19), hydroxytetramethoxyflavone (20, 41), hydroxyhexametoxyflavone ( 21, 24, 39 and 45), dihydroxytetramethoxyflavone (23, 34), sinensetin (26), dihymethoxyflavone (27), tetramethyl-O-isoscutellarein (29), pentamethoxyflavone (30), dihydroxytrimethoxyflavone (31), tryhydroxytetramethoxyflavone (32), tetramethoxyflavone (33), 3,3’,4’,5,6,7,8-heptamethoxyflavone (37), hydroxypentamethoxyflavone (38, 45), 3-hydroxy-hexamethoxyflavone (39), trimethoxyflavone (40), and 3-hydroxy-pentamethoxyflavone (44), hesperetin 7-O-glucoside (48) and hesperetin (49). Total phenol contents of the nine citrus varieties were measured by Folin-Denis method. The mean content was 2.90mg/g. The content was lowest for Citrus leiocarpa Hort. et Tanaka (1.98±0.13mg/g), and highest for Citrus nippokoreana (4.62±0.13mg/g). The antioxidant effect was evaluated for the flavonoids of the nine citrus varieties; DPPH, ABTS, NO, FRAP. The effect showed dose-dependant trend for the all tests. The identified compounds from fruit peel of C. unshiu Marc. Citrus erythrosa Hort, Citrus sunki Hort, Citrus benlkoji Hort, Citrus avrantium L., Citrus leiocarpa Hort. et Tanaka, Citrus playmamma Hort, Citrus nippokoreana and Citrus natsudaidai Hayata were quantified by HPLC. To investigate the function of the flavonoids as defense materials, the flavonoid content change of the fruit peel of inoculated with P. digitatum was monitored by HPLC. The flavonoid concentration in the infected fruit peel decreased initially after the infection, but then gradually increased before finally progressively decreasing.
Fruit peels of Citrus unshiu Marc., Citrus erythrosa Hort, Citrus sunki Hort, Citrus benlkoji Hort, Citrus avrantium L., Citrus leiocarpa Hort. et Tanaka, Citrus playmamma Hort, Citrus nippokoreana and Citrus natsudaidai Hayata were analyzed for flavonoids via high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) in the positive mode with selected ion monitoring (SIM). Among fortynine flavonoid components labeled, five flavanones, twenty tow flavones and tow coumarins were identified for the first time; naringin (3), poncirin (9), trihydroxytetramethoxyflavone-glucoside (11), meranzin (15), hexamethoxyflavone isomers (12, 25, 28 and 45), hydroxypentamethoxyflavone (16, 22), pentahydroxymethoxyflavone (17), isomeranzin (18), isosinensetin (19), hydroxytetramethoxyflavone (20, 41), hydroxyhexametoxyflavone ( 21, 24, 39 and 45), dihydroxytetramethoxyflavone (23, 34), sinensetin (26), dihymethoxyflavone (27), tetramethyl-O-isoscutellarein (29), pentamethoxyflavone (30), dihydroxytrimethoxyflavone (31), tryhydroxytetramethoxyflavone (32), tetramethoxyflavone (33), 3,3’,4’,5,6,7,8-heptamethoxyflavone (37), hydroxypentamethoxyflavone (38, 45), 3-hydroxy-hexamethoxyflavone (39), trimethoxyflavone (40), and 3-hydroxy-pentamethoxyflavone (44), hesperetin 7-O-glucoside (48) and hesperetin (49). Total phenol contents of the nine citrus varieties were measured by Folin-Denis method. The mean content was 2.90mg/g. The content was lowest for Citrus leiocarpa Hort. et Tanaka (1.98±0.13mg/g), and highest for Citrus nippokoreana (4.62±0.13mg/g). The antioxidant effect was evaluated for the flavonoids of the nine citrus varieties; DPPH, ABTS, NO, FRAP. The effect showed dose-dependant trend for the all tests. The identified compounds from fruit peel of C. unshiu Marc. Citrus erythrosa Hort, Citrus sunki Hort, Citrus benlkoji Hort, Citrus avrantium L., Citrus leiocarpa Hort. et Tanaka, Citrus playmamma Hort, Citrus nippokoreana and Citrus natsudaidai Hayata were quantified by HPLC. To investigate the function of the flavonoids as defense materials, the flavonoid content change of the fruit peel of inoculated with P. digitatum was monitored by HPLC. The flavonoid concentration in the infected fruit peel decreased initially after the infection, but then gradually increased before finally progressively decreasing.
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#감귤 플라보노이드 HPLC-MS/MS
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