Paper is composed of organic materials like cellulosic fibers and organic chemical additives. These components are combined by the mechanism of hydrogen bonding, ionic bonding and Van Der Vaal's force. However, paper can be easily degraded by different internal and external factors, called "paper ag...
Paper is composed of organic materials like cellulosic fibers and organic chemical additives. These components are combined by the mechanism of hydrogen bonding, ionic bonding and Van Der Vaal's force. However, paper can be easily degraded by different internal and external factors, called "paper ageing" phenomenon. Internal factors are mainly related to the manufacturing processes and constituents. External factors depend on light, temperature, oxygen, humidity changes, insects and microorganisms. Most of all, microorganisms have a bad effects, such as the decrease of molecular weight, foxing, discoloration, nasty smell and risk for human health. When the microorganisms grow up in paper, cellulose degradation is occurred by multi-enzymatic mechanism involved of three activities : endo-1,4-β-glucanase, cellobiohydrolase, and β-glucosidase.
Accordingly it is important to identify paper aging mechanism related to biodegradation. In this study, some fungus were isolated from old books and shelves at University library in Seoul, Korea. 85 kinds of fungus were totally isolated and their Information on individual fungi were obtained by using DNA- based method. In total 85 kinds of fungi, HJF007, HJF031 and HJF085, which have high cellulase activity were screened. In order to investigate the biodegradable factors affecting on papers, the 3 kinds of screened fungi and 2 kinds of controlled fungi, A. niger, and T. viridae were inoculated in 4 kinds of paper sample, which are Whatman filter paper, Korean traditional paper(Hanji), conservation paper, and fine paper. Growth rate and reducing sugar were analysed after the biodegradation of paper samples by fungi.
As the results, the enzymatic activities of HJF007, HJF031, and HJF085 was higher than other 5 kinds of fungi. Growth rate of all fungus was especially higher on Hanji sample. Reducing sugar was high in HJF007, HJF031 on fine paper, and high in HJF085 on filter paper, but low in 5 kinds of fungi on Hanji. The enzymatic activities of 5 kinds of fungi was increased by the addition of filtrate screened from other 3 kinds of paper with 200 mesh wire after disintegration, but in the case of Hanji, was similar compared to the condition by the addition of deionized water. Reducing sugar in fibrous materials was highest on Whatman filter paper, but was lowest on Hanji. I can be also concluded that 3 kinds of screened fungi from university library would have bad affection in the enzymatic degradation of papers, compared to 2 kinds of controlled fungi.
Paper is composed of organic materials like cellulosic fibers and organic chemical additives. These components are combined by the mechanism of hydrogen bonding, ionic bonding and Van Der Vaal's force. However, paper can be easily degraded by different internal and external factors, called "paper ageing" phenomenon. Internal factors are mainly related to the manufacturing processes and constituents. External factors depend on light, temperature, oxygen, humidity changes, insects and microorganisms. Most of all, microorganisms have a bad effects, such as the decrease of molecular weight, foxing, discoloration, nasty smell and risk for human health. When the microorganisms grow up in paper, cellulose degradation is occurred by multi-enzymatic mechanism involved of three activities : endo-1,4-β-glucanase, cellobiohydrolase, and β-glucosidase.
Accordingly it is important to identify paper aging mechanism related to biodegradation. In this study, some fungus were isolated from old books and shelves at University library in Seoul, Korea. 85 kinds of fungus were totally isolated and their Information on individual fungi were obtained by using DNA- based method. In total 85 kinds of fungi, HJF007, HJF031 and HJF085, which have high cellulase activity were screened. In order to investigate the biodegradable factors affecting on papers, the 3 kinds of screened fungi and 2 kinds of controlled fungi, A. niger, and T. viridae were inoculated in 4 kinds of paper sample, which are Whatman filter paper, Korean traditional paper(Hanji), conservation paper, and fine paper. Growth rate and reducing sugar were analysed after the biodegradation of paper samples by fungi.
As the results, the enzymatic activities of HJF007, HJF031, and HJF085 was higher than other 5 kinds of fungi. Growth rate of all fungus was especially higher on Hanji sample. Reducing sugar was high in HJF007, HJF031 on fine paper, and high in HJF085 on filter paper, but low in 5 kinds of fungi on Hanji. The enzymatic activities of 5 kinds of fungi was increased by the addition of filtrate screened from other 3 kinds of paper with 200 mesh wire after disintegration, but in the case of Hanji, was similar compared to the condition by the addition of deionized water. Reducing sugar in fibrous materials was highest on Whatman filter paper, but was lowest on Hanji. I can be also concluded that 3 kinds of screened fungi from university library would have bad affection in the enzymatic degradation of papers, compared to 2 kinds of controlled fungi.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.