초고압 추출에 따른 도라지의 추출수율, 조사포닌 그리고 platycoside에 대한 변화를 조사해 본 결과는 다음과 같다. 도라지 사포닌인 platycoside 추출에 있어서 그 solubility가 제일 높은 추출용매는 70% ethanol-water mixture(v/v)임을 알 수 있었다. 특히 용매의 ethanol 농도가 높아짐에 따라 추출수율은 감소하는 것이 관찰되었다. 1:10, 1:20, 1:30의 다양한 용질/용매 비율에서 실험한 결과, mass transfer는 1:20에서부터 유의적 차이를 보이지 않음을 알 수 있었다. 이는 이 비율에서 추출용매가 충분히 세포 안으로 침투하여 세포 안/밖에서 평형상태(equilibrium state)가 된 것으로 생각된다. ...
초고압 추출에 따른 도라지의 추출수율, 조사포닌 그리고 platycoside에 대한 변화를 조사해 본 결과는 다음과 같다. 도라지 사포닌인 platycoside 추출에 있어서 그 solubility가 제일 높은 추출용매는 70% ethanol-water mixture(v/v)임을 알 수 있었다. 특히 용매의 ethanol 농도가 높아짐에 따라 추출수율은 감소하는 것이 관찰되었다. 1:10, 1:20, 1:30의 다양한 용질/용매 비율에서 실험한 결과, mass transfer는 1:20에서부터 유의적 차이를 보이지 않음을 알 수 있었다. 이는 이 비율에서 추출용매가 충분히 세포 안으로 침투하여 세포 안/밖에서 평형상태(equilibrium state)가 된 것으로 생각된다. 반응표면분석 결과, 추출수율은 추출시간보다는 추출압력에 더 의존적으로 증가하는 것을 알 수 있었으며, 조사포닌 함량의 반응표면모델식은 그 p-value가 다소 높아 유의성이 떨어지는 반응표면모델식을 얻었다. 추출수율과 조사포닌을 가장 높게 얻을 수 있었던 최적조건은 450 MPa의 압력과 5 min의 추출시간이었으며, 검증실험을 한 결과 95% 신뢰구간 안에 들어가는 값을 얻을 수 있었다. 초고압 추출법과 기존의 추출법을 비교한 결과, 추출수율은 초고압 추출, 조사포닌 함량은 환류추출, Total platycoside는 초음파추출과 고전압 펄스 전기장 추출에서 각각 높은 값을 나타냈다. 초고압 추출은 도라지의 platycoside 추출에 있어서 추출시간과 에너지 소비를 줄이는 장점이 있었다. 특히 본 연구에서는 도라지의 사포닌만을 분석하였지만 높은 열에 다소 민감한 vitamin 종류나 phytochemical, 향기성분 등의 추출에 사용하면 더 큰 효과가 있을 것이라고 생각된다.
초고압 추출에 따른 도라지의 추출수율, 조사포닌 그리고 platycoside에 대한 변화를 조사해 본 결과는 다음과 같다. 도라지 사포닌인 platycoside 추출에 있어서 그 solubility가 제일 높은 추출용매는 70% ethanol-water mixture(v/v)임을 알 수 있었다. 특히 용매의 ethanol 농도가 높아짐에 따라 추출수율은 감소하는 것이 관찰되었다. 1:10, 1:20, 1:30의 다양한 용질/용매 비율에서 실험한 결과, mass transfer는 1:20에서부터 유의적 차이를 보이지 않음을 알 수 있었다. 이는 이 비율에서 추출용매가 충분히 세포 안으로 침투하여 세포 안/밖에서 평형상태(equilibrium state)가 된 것으로 생각된다. 반응표면분석 결과, 추출수율은 추출시간보다는 추출압력에 더 의존적으로 증가하는 것을 알 수 있었으며, 조사포닌 함량의 반응표면모델식은 그 p-value가 다소 높아 유의성이 떨어지는 반응표면모델식을 얻었다. 추출수율과 조사포닌을 가장 높게 얻을 수 있었던 최적조건은 450 MPa의 압력과 5 min의 추출시간이었으며, 검증실험을 한 결과 95% 신뢰구간 안에 들어가는 값을 얻을 수 있었다. 초고압 추출법과 기존의 추출법을 비교한 결과, 추출수율은 초고압 추출, 조사포닌 함량은 환류추출, Total platycoside는 초음파추출과 고전압 펄스 전기장 추출에서 각각 높은 값을 나타냈다. 초고압 추출은 도라지의 platycoside 추출에 있어서 추출시간과 에너지 소비를 줄이는 장점이 있었다. 특히 본 연구에서는 도라지의 사포닌만을 분석하였지만 높은 열에 다소 민감한 vitamin 종류나 phytochemical, 향기성분 등의 추출에 사용하면 더 큰 효과가 있을 것이라고 생각된다.
Effective components of Platycodon grandiflorum A. DC. are known as Platycosides, which is it’s saponin. Ultrahigh pressure extraction (UHPE) has many benefits for extraction of heat labile bioactive components. In this study, optimization of UHPE of P. grandiflorum A. DC. powder has been investigat...
Effective components of Platycodon grandiflorum A. DC. are known as Platycosides, which is it’s saponin. Ultrahigh pressure extraction (UHPE) has many benefits for extraction of heat labile bioactive components. In this study, optimization of UHPE of P. grandiflorum A. DC. powder has been investigated. The powder has been extracted with various ethanol concentrations under UHP and compared with conventional extraction methods. Optimum conditions of UHPE were evaluated for Platycodi radix (root of platycodon) powder using response surface methodology (RSM). Pressure (X1) and time (X2) were chosen as independent variables under the central composite design (CCD). In case of UHPE (at 25 ℃, 200 MPa for 5 min), extraction yield decreased with increasing ethanol concentration and crude saponin contents increased up to 70% ethanol-water mixture (v/v) and decreased thereafter. The highest extraction yield and crude saponin contents were obtained at 0% ethanol and 70% ethanol, respectively. And, when solute/solvent ratio was used 1:20 (w/v), extraction yield with crude saponin contents were shown highest values. UHPE of Platycodi radix powder was performed using 70% ethanol-water mixture (v/v) under different pressures (200-450 MPa) and times (5-15 min). The extraction effect was indicated as extraction yield (%, Y1) and crude saponin contents (mg/g, Y2). The optimum conditions of highest extraction yield with/without crude saponin contents were obtained at 450 MPa for 5 min. Desirabilities were 0.788 and 0.949, respectively. And predicted values were 39.52% and 13.07 mg/g solute, respectively. Besides two response was considered, that made desirability decreased (0.788). The remarkable characteristic was that extraction yield increased with increasing pressure regardless of extraction time. On the other hand, as pressure and time increased, crude saponin contents varied insignificantly indicating that crude saponin was sufficiently extracted at relatively low pressure (200 MPa). Also UHPE was compared with other extraction methods, such as heat reflux extraction (HRE), ultrasound-assisted extraction (UAE), soxhlet extraction (SE), microwave-assisted extraction (MAE) and pulsed electric field extraction (PEFE). The platycosides were quantified by HPLC equipped with UV detector. Compared with other extraction methods, highest extraction yield could be obtained at UHPE (water, 450 MPa, 5 min). And highest crude saponins could be obtained at HRE (70% ethanol, 12 hr) and highest total platycosides could be obtained at UAE (water, 1 hr) and PEFE (water, 21.4 L/hr). It could be known that UHPE took relatively low extraction time, energy spending than any other methods.
Effective components of Platycodon grandiflorum A. DC. are known as Platycosides, which is it’s saponin. Ultrahigh pressure extraction (UHPE) has many benefits for extraction of heat labile bioactive components. In this study, optimization of UHPE of P. grandiflorum A. DC. powder has been investigated. The powder has been extracted with various ethanol concentrations under UHP and compared with conventional extraction methods. Optimum conditions of UHPE were evaluated for Platycodi radix (root of platycodon) powder using response surface methodology (RSM). Pressure (X1) and time (X2) were chosen as independent variables under the central composite design (CCD). In case of UHPE (at 25 ℃, 200 MPa for 5 min), extraction yield decreased with increasing ethanol concentration and crude saponin contents increased up to 70% ethanol-water mixture (v/v) and decreased thereafter. The highest extraction yield and crude saponin contents were obtained at 0% ethanol and 70% ethanol, respectively. And, when solute/solvent ratio was used 1:20 (w/v), extraction yield with crude saponin contents were shown highest values. UHPE of Platycodi radix powder was performed using 70% ethanol-water mixture (v/v) under different pressures (200-450 MPa) and times (5-15 min). The extraction effect was indicated as extraction yield (%, Y1) and crude saponin contents (mg/g, Y2). The optimum conditions of highest extraction yield with/without crude saponin contents were obtained at 450 MPa for 5 min. Desirabilities were 0.788 and 0.949, respectively. And predicted values were 39.52% and 13.07 mg/g solute, respectively. Besides two response was considered, that made desirability decreased (0.788). The remarkable characteristic was that extraction yield increased with increasing pressure regardless of extraction time. On the other hand, as pressure and time increased, crude saponin contents varied insignificantly indicating that crude saponin was sufficiently extracted at relatively low pressure (200 MPa). Also UHPE was compared with other extraction methods, such as heat reflux extraction (HRE), ultrasound-assisted extraction (UAE), soxhlet extraction (SE), microwave-assisted extraction (MAE) and pulsed electric field extraction (PEFE). The platycosides were quantified by HPLC equipped with UV detector. Compared with other extraction methods, highest extraction yield could be obtained at UHPE (water, 450 MPa, 5 min). And highest crude saponins could be obtained at HRE (70% ethanol, 12 hr) and highest total platycosides could be obtained at UAE (water, 1 hr) and PEFE (water, 21.4 L/hr). It could be known that UHPE took relatively low extraction time, energy spending than any other methods.
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