This study was carried out to establish a micropropagation method of Vaccinium oldhamii Miquel. Material plants, about 10 years old, were moved to a greenhouse of Chungbuk Forest Environment Institute from natural habitat in April 2010. 1. Shoots with nodes were cultured on WPM medium without growth...
This study was carried out to establish a micropropagation method of Vaccinium oldhamii Miquel. Material plants, about 10 years old, were moved to a greenhouse of Chungbuk Forest Environment Institute from natural habitat in April 2010. 1. Shoots with nodes were cultured on WPM medium without growth regulators with 55% success, and on MS medium with no growth regulators with 33% success. From leaf segments, 1 cm × 1 cm size, of in vitro shoots callus were tried to induce on WPM with 5 levels of concentrations of 2,4-D, 0, 0.1, 0.3, 0.5, 1.0 mg/L Hundred percent of leaf segments produced calli with 0.5 mg/L 2,4-D added to WPM after 3 weeks of culture. 2. For shoot regeneration from callus, 0.5 mg/l zeatin added to WPM showed the highest rate of 66% among 0, 0.1, 0.3, 0.5, 1.0, 2.0 mg/L whereas 0.3 mg/L TDZ added to same basal medium did 44% regeneration rate among 0.01, 0.03, 0.05, 0.1, 0.3 mg/L and whereas 1.0 mg/L 2,4-D did the 25% among 0, 0.1, 0.3, 0.5, 1.0 mg/L and whereas 0.3 mg/L and 0.5 mg/L did 33% rate among 0, 0.1, 0.3, 0.5, 2.0 mg/L after 60 days of culture, respectively. Combinatorial addition of 0.1 mg/L 2,4-D + zeatin 0.5 mg/L and 0.1 mg/L 2,4-D + zeatin 0.5 mg/L showed 0% and 22%, respectively. 3. For rooting of microshoots on medium, among different concentrations of IBA added to 1/2 GD, 0.3 mg/L showed the highest rate of callus induction 88% after 4 weeks of culture. From further culture of the callus induced on the same media without subculture for 6 weeks, of 62.5% of root induction were achieved on 0.3 mg/L added to IBA. In case of NAA concentration, 1.5 mg/L showed the same results as 0.3 mg/L IBA as mentioned above. As histological analysis showed that the rooting by hormonal treatment is indirect, Rooting was examined with Gippy pot. Rooting of microshoot cuts conducted on Gippy pot in a culture bottle in culture room with dipping the base of microshoots into 500 ppm IBA solution for 10 minutes showed 100% rooting rate after 4 weeks. 4. For acclimatization of plantlets as with rooting of cuttings Gippy pots were used. Rooted plantlets were grown in Gippy pots in a culture bottle in culture room for 1 month and transferred to pot and grown in a greenhouse for another 1 month. All the plantlets survived in Gippy pots and in the greenhouse.
This study was carried out to establish a micropropagation method of Vaccinium oldhamii Miquel. Material plants, about 10 years old, were moved to a greenhouse of Chungbuk Forest Environment Institute from natural habitat in April 2010. 1. Shoots with nodes were cultured on WPM medium without growth regulators with 55% success, and on MS medium with no growth regulators with 33% success. From leaf segments, 1 cm × 1 cm size, of in vitro shoots callus were tried to induce on WPM with 5 levels of concentrations of 2,4-D, 0, 0.1, 0.3, 0.5, 1.0 mg/L Hundred percent of leaf segments produced calli with 0.5 mg/L 2,4-D added to WPM after 3 weeks of culture. 2. For shoot regeneration from callus, 0.5 mg/l zeatin added to WPM showed the highest rate of 66% among 0, 0.1, 0.3, 0.5, 1.0, 2.0 mg/L whereas 0.3 mg/L TDZ added to same basal medium did 44% regeneration rate among 0.01, 0.03, 0.05, 0.1, 0.3 mg/L and whereas 1.0 mg/L 2,4-D did the 25% among 0, 0.1, 0.3, 0.5, 1.0 mg/L and whereas 0.3 mg/L and 0.5 mg/L did 33% rate among 0, 0.1, 0.3, 0.5, 2.0 mg/L after 60 days of culture, respectively. Combinatorial addition of 0.1 mg/L 2,4-D + zeatin 0.5 mg/L and 0.1 mg/L 2,4-D + zeatin 0.5 mg/L showed 0% and 22%, respectively. 3. For rooting of microshoots on medium, among different concentrations of IBA added to 1/2 GD, 0.3 mg/L showed the highest rate of callus induction 88% after 4 weeks of culture. From further culture of the callus induced on the same media without subculture for 6 weeks, of 62.5% of root induction were achieved on 0.3 mg/L added to IBA. In case of NAA concentration, 1.5 mg/L showed the same results as 0.3 mg/L IBA as mentioned above. As histological analysis showed that the rooting by hormonal treatment is indirect, Rooting was examined with Gippy pot. Rooting of microshoot cuts conducted on Gippy pot in a culture bottle in culture room with dipping the base of microshoots into 500 ppm IBA solution for 10 minutes showed 100% rooting rate after 4 weeks. 4. For acclimatization of plantlets as with rooting of cuttings Gippy pots were used. Rooted plantlets were grown in Gippy pots in a culture bottle in culture room for 1 month and transferred to pot and grown in a greenhouse for another 1 month. All the plantlets survived in Gippy pots and in the greenhouse.
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