저비용 간이 수경재배 시스템을 이용한 고구마 바이러스 무병묘 대량증식 체계를 확립하기 위하여, NFT와 자루재배 방식으로 무병묘 번식시험을 실시하였다. NFT 방식은 버미큐라이트와 펄라이트(1:1)를 채운 72공 플러그 트레이(35mL)에 무병주를 삽식하여 양액종류, 삽수...
저비용 간이 수경재배 시스템을 이용한 고구마 바이러스 무병묘 대량증식 체계를 확립하기 위하여, NFT와 자루재배 방식으로 무병묘 번식시험을 실시하였다. NFT 방식은 버미큐라이트와 펄라이트(1:1)를 채운 72공 플러그 트레이(35mL)에 무병주를 삽식하여 양액종류, 삽수크기(1마디, 2마디, 3마디, 정단)와 품종 (‘맛나미’, ‘신천미’, ‘신자미’, ‘연황미’)에 따른 생육특성을 조사하였다. 자루재배는 점적호스를 이용한 순환식 자루재배 방식을 이용하여 배지 혼합 비율(cocopeat+perlite/3:7, 5:5, 7:3, v/v), 삽수종류(1마디 및 정단) 및 품종(‘맛나미’, ‘연황미’)에 따른 무병묘의 생육특성을 비교하였다.
1. 무병묘의 줄기신장, 생체중 및 건물중 등은 일본원시액>표준원시액>야마자키 상추액 순으로 양호한 생육을 보였으며, 야마자키 상추액보다 일본원시액에서 유의하게 증가하였다.
2. 삽수크기에 따른 무병묘 생육은 삽수크기가 클수록 줄기길이, 생체중 및 건물중 등이 양호하였으며, 정단삽수와 3마디 삽식에서 양호하였다. 품종간에는 ‘신자미’와 ‘연황미’의 줄기신장, 생체중 및 건물중 증가가 양호하였다. 대량증식 효율은 1마디 삽수를 이용하는 것이 경제적이었다.
3. 플러그 셀 크기에 따른 무병묘 생육은 밀식할수록 줄기신장을 제외한 묘소질이 좋지 않았으며, 우량묘 생산을 위해서는 72공 또는 105공 플러그가 적합하였다. 종순생산(25cm 이상)은 1마디 삽식으로 72공 플러그에서 25일간 배양하고, 농가 분양묘(10cm 이상)는 105공 플러그에서 15일간 재배하여 생산하는 것이 경제적이었다.
4. 삽수종류에 따른 무병묘의 자루재배에서 정단 삽수의 줄기신장이 1마디 삽수보다 유의한 증가를 보였다. 생체중 및 건물중은 코코피트와 펄라이트 3:7(v/v) 혼합배지에서 코코피트와 펄라이트 7:3, (v/v) 혼합배지에서보다 유의하게 증가하였다. 따라서 코코피트와 펄라이트(3:7, v/v) 혼합배지를 이용한 자루재배는 고구마 무병묘 증식에 성공적으로 적용할 수 있었으며, 고구마 종순농가에서도 큰 경제적 부담 없이 적용할 수 있을 것으로 기대되었다.
저비용 간이 수경재배 시스템을 이용한 고구마 바이러스 무병묘 대량증식 체계를 확립하기 위하여, NFT와 자루재배 방식으로 무병묘 번식시험을 실시하였다. NFT 방식은 버미큐라이트와 펄라이트(1:1)를 채운 72공 플러그 트레이(35mL)에 무병주를 삽식하여 양액종류, 삽수크기(1마디, 2마디, 3마디, 정단)와 품종 (‘맛나미’, ‘신천미’, ‘신자미’, ‘연황미’)에 따른 생육특성을 조사하였다. 자루재배는 점적호스를 이용한 순환식 자루재배 방식을 이용하여 배지 혼합 비율(cocopeat+perlite/3:7, 5:5, 7:3, v/v), 삽수종류(1마디 및 정단) 및 품종(‘맛나미’, ‘연황미’)에 따른 무병묘의 생육특성을 비교하였다.
1. 무병묘의 줄기신장, 생체중 및 건물중 등은 일본원시액>표준원시액>야마자키 상추액 순으로 양호한 생육을 보였으며, 야마자키 상추액보다 일본원시액에서 유의하게 증가하였다.
2. 삽수크기에 따른 무병묘 생육은 삽수크기가 클수록 줄기길이, 생체중 및 건물중 등이 양호하였으며, 정단삽수와 3마디 삽식에서 양호하였다. 품종간에는 ‘신자미’와 ‘연황미’의 줄기신장, 생체중 및 건물중 증가가 양호하였다. 대량증식 효율은 1마디 삽수를 이용하는 것이 경제적이었다.
3. 플러그 셀 크기에 따른 무병묘 생육은 밀식할수록 줄기신장을 제외한 묘소질이 좋지 않았으며, 우량묘 생산을 위해서는 72공 또는 105공 플러그가 적합하였다. 종순생산(25cm 이상)은 1마디 삽식으로 72공 플러그에서 25일간 배양하고, 농가 분양묘(10cm 이상)는 105공 플러그에서 15일간 재배하여 생산하는 것이 경제적이었다.
4. 삽수종류에 따른 무병묘의 자루재배에서 정단 삽수의 줄기신장이 1마디 삽수보다 유의한 증가를 보였다. 생체중 및 건물중은 코코피트와 펄라이트 3:7(v/v) 혼합배지에서 코코피트와 펄라이트 7:3, (v/v) 혼합배지에서보다 유의하게 증가하였다. 따라서 코코피트와 펄라이트(3:7, v/v) 혼합배지를 이용한 자루재배는 고구마 무병묘 증식에 성공적으로 적용할 수 있었으며, 고구마 종순농가에서도 큰 경제적 부담 없이 적용할 수 있을 것으로 기대되었다.
This study was carried out to establish the mass-propagation system of sweet potato [Ipomoea batatas (L.) Lam.] virus-free seedling using nutrient film technique (NFT) and bag culture for simple and low-cost hydroponics. In NFT system, growth characteristics as affected by nutrient solutions (NHRSJ,...
This study was carried out to establish the mass-propagation system of sweet potato [Ipomoea batatas (L.) Lam.] virus-free seedling using nutrient film technique (NFT) and bag culture for simple and low-cost hydroponics. In NFT system, growth characteristics as affected by nutrient solutions (NHRSJ, NHRIK and Yamazaki's lettuce solution), cutting sizes (1-, 2-, 3-nodes, and shoot-tip) and varieties ('Shinzami', 'Yeonhwangmi', 'Mannami' and 'Shincheonmi') were investigated in 72 cells-plug trays (35mL) filled with vermiculite and perlite (1:1, v/v). In bag culture, growth characteristics as affected by media, cutting sources (one-node and shoot-tip) and varieties ('Yeonhwangmi' and 'Mannami') were investigated in the plastic bag (0.5mm of thickness, W 300×L 1,200×H 120mm) culture system (connected the hose drip-irrigation system) filled with commercial horticultural media (TKS-2, Flora Gard Co.) and cocopeat + perlite (3:7, 5:5, 7:3, v/v).
1. Vine length, fresh and dry weights of virus-free seedlings were greatest in the nutrient solution recommended by National Horticultural Research station in Japan (NHRSJ), followed by the nutrient solution recommended by National Horticultural Research Institute in Korea (NHRIK), and Yamazaki's lettuce solution. These were significantly higher in the nutrient solution of NHRSJ than those in Yamazaki's lettuce solution.
2. The growth of shoot-tip and 3-node cuttings was excellent among 4 kinds of cuttings, and vine length, fresh and dry weights were increased in longer cuttings. Vine length, fresh and dry weights were increased in 'Shinzami' and 'Yeonhwangmi' than those in 'Mannami' and 'Shincheonmi'. The efficiency of mass-propagation was higher in single node cutting compared to the 2- or 3- nodes cuttings.
3. The effect of plug cell size on seedling quality was not enough in smaller plug cell size, but vine length showed fast growth in bigger plug cell size. The seedling quality in 72 and 105 cell trays were suitable for virus-free seedling production based on economic view. The full-grown seedling (over 25cm) could be produced using single-node cutting for 25 days in 72 cell trays, and the seedling provided to farmers could be produced by cultivating for 15 days in 105 cell trays.
4. Shoot-tip cutting showed a significant vine elongation compared to the one-node cutting. Fresh weight and dry weight in cocopeat 3:perlite 7(v/v) were significant increased than that in cocopeat 7:perlite 3(v/v). The bag culture system filed with cocopeat 3:perlite 7(v/v) was successfully applied on propagation of sweet potato virus-free plant, and the result expected that the bag culture system could be commercialized without high financial costs for farmers.
This study was carried out to establish the mass-propagation system of sweet potato [Ipomoea batatas (L.) Lam.] virus-free seedling using nutrient film technique (NFT) and bag culture for simple and low-cost hydroponics. In NFT system, growth characteristics as affected by nutrient solutions (NHRSJ, NHRIK and Yamazaki's lettuce solution), cutting sizes (1-, 2-, 3-nodes, and shoot-tip) and varieties ('Shinzami', 'Yeonhwangmi', 'Mannami' and 'Shincheonmi') were investigated in 72 cells-plug trays (35mL) filled with vermiculite and perlite (1:1, v/v). In bag culture, growth characteristics as affected by media, cutting sources (one-node and shoot-tip) and varieties ('Yeonhwangmi' and 'Mannami') were investigated in the plastic bag (0.5mm of thickness, W 300×L 1,200×H 120mm) culture system (connected the hose drip-irrigation system) filled with commercial horticultural media (TKS-2, Flora Gard Co.) and cocopeat + perlite (3:7, 5:5, 7:3, v/v).
1. Vine length, fresh and dry weights of virus-free seedlings were greatest in the nutrient solution recommended by National Horticultural Research station in Japan (NHRSJ), followed by the nutrient solution recommended by National Horticultural Research Institute in Korea (NHRIK), and Yamazaki's lettuce solution. These were significantly higher in the nutrient solution of NHRSJ than those in Yamazaki's lettuce solution.
2. The growth of shoot-tip and 3-node cuttings was excellent among 4 kinds of cuttings, and vine length, fresh and dry weights were increased in longer cuttings. Vine length, fresh and dry weights were increased in 'Shinzami' and 'Yeonhwangmi' than those in 'Mannami' and 'Shincheonmi'. The efficiency of mass-propagation was higher in single node cutting compared to the 2- or 3- nodes cuttings.
3. The effect of plug cell size on seedling quality was not enough in smaller plug cell size, but vine length showed fast growth in bigger plug cell size. The seedling quality in 72 and 105 cell trays were suitable for virus-free seedling production based on economic view. The full-grown seedling (over 25cm) could be produced using single-node cutting for 25 days in 72 cell trays, and the seedling provided to farmers could be produced by cultivating for 15 days in 105 cell trays.
4. Shoot-tip cutting showed a significant vine elongation compared to the one-node cutting. Fresh weight and dry weight in cocopeat 3:perlite 7(v/v) were significant increased than that in cocopeat 7:perlite 3(v/v). The bag culture system filed with cocopeat 3:perlite 7(v/v) was successfully applied on propagation of sweet potato virus-free plant, and the result expected that the bag culture system could be commercialized without high financial costs for farmers.
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