Background: Rapid laboratory methods provide optimal support for active surveillance efforts to screen for methicillin resistant Staphylococcus aureus (MRSA). To assess the performance of common methods, comparison of between MSO MRSA, ChromID MRSA (BioMerieux, Marcy I’Etoile, France), Oxoid Brillan...
Background: Rapid laboratory methods provide optimal support for active surveillance efforts to screen for methicillin resistant Staphylococcus aureus (MRSA). To assess the performance of common methods, comparison of between MSO MRSA, ChromID MRSA (BioMerieux, Marcy I’Etoile, France), Oxoid Brillance MRSA (Oxoid, Basingstoke Hants, UK.) selective agars and BD GeneOhm MRSA PCR assay (Becton Dickinson, San Diego, CA, USA)
Methods: A total of 187 clinical samples were collected from the anterior nares of patients in intensive care unit (ICU). One of the swab was inoculated on mannitol salt agar with oxacillin 6 mg/L (MSO) MRSA and BD GeneOhm MRSA PCR assay, the other swab was inoculated directly ChromID MRSA and Oxoid Brillance MRSA.
Results: Sixty-one of 187 samples(32.6%) was positive for MRSA by Gram Stain, coagulase test and disk-diffusion test. After 24h incubation, the sensitivity, specificity of MSO MRSA was 56.5%, 98.6%, ChromID MRSA was 88.9%, 98.6%, and that of Oxoid Brillance MRSA was 89.1%, 99.3%. At 48h, the sensitivity, specificity of MSO MRSA was 78.0%, 98.4%, ChromID MRSA was 85.0%, 99.2% and that of Oxoid Brillance MRSA was 93.3%, 99.2%. The sensitivity, specificity BD GeneOhm MRSA PCR assay was 77.3%, 84.3%,
Conclusion: Selective agars are useful for the rapid isolation and identification of MRSA with Gram stain, coagulase test, disk-diffusion test.
Background: Rapid laboratory methods provide optimal support for active surveillance efforts to screen for methicillin resistant Staphylococcus aureus (MRSA). To assess the performance of common methods, comparison of between MSO MRSA, ChromID MRSA (BioMerieux, Marcy I’Etoile, France), Oxoid Brillance MRSA (Oxoid, Basingstoke Hants, UK.) selective agars and BD GeneOhm MRSA PCR assay (Becton Dickinson, San Diego, CA, USA)
Methods: A total of 187 clinical samples were collected from the anterior nares of patients in intensive care unit (ICU). One of the swab was inoculated on mannitol salt agar with oxacillin 6 mg/L (MSO) MRSA and BD GeneOhm MRSA PCR assay, the other swab was inoculated directly ChromID MRSA and Oxoid Brillance MRSA.
Results: Sixty-one of 187 samples(32.6%) was positive for MRSA by Gram Stain, coagulase test and disk-diffusion test. After 24h incubation, the sensitivity, specificity of MSO MRSA was 56.5%, 98.6%, ChromID MRSA was 88.9%, 98.6%, and that of Oxoid Brillance MRSA was 89.1%, 99.3%. At 48h, the sensitivity, specificity of MSO MRSA was 78.0%, 98.4%, ChromID MRSA was 85.0%, 99.2% and that of Oxoid Brillance MRSA was 93.3%, 99.2%. The sensitivity, specificity BD GeneOhm MRSA PCR assay was 77.3%, 84.3%,
Conclusion: Selective agars are useful for the rapid isolation and identification of MRSA with Gram stain, coagulase test, disk-diffusion test.
Keyword
#Methicillin-resistant Staphylococcus aureus(MRSA)
#selective chrome agar
#BD GeneOhm MRSA PCR
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