인삼의 증숙 횟수와 농축조건에 따른 진세노사이드 함량 변화와 항산화, 항암 활성 Changes in Ginsenoside contents, Antioxidant and Anticancer Activities According to Steaming Condition in Ginseng원문보기
흑삼 제조 과정에서 증숙 횟수 별, 온도, 시간차에 따른 성분 변화를 분석하여 흑삼 제조의 표준화를 규명하고, 항산화와 항암활성을 검정하여 약리효과에 대한 기초자료를 얻고자 수행 하였다. 1. 98℃로 6시간씩 아홉 번 증숙하여 건조시킨 흑삼의 조건별 Ginseno side 성분을 분석한 결과, protopanaxadiol(PPD)계 ginsenoside인 Rb1, Rb2, Rc, Rd는 증숙 처리를 거치면서 함량이 감소한 반면 Rg3는 크게 증가하였다. 또한 protopanaxtriol(...
흑삼 제조 과정에서 증숙 횟수 별, 온도, 시간차에 따른 성분 변화를 분석하여 흑삼 제조의 표준화를 규명하고, 항산화와 항암활성을 검정하여 약리효과에 대한 기초자료를 얻고자 수행 하였다. 1. 98℃로 6시간씩 아홉 번 증숙하여 건조시킨 흑삼의 조건별 Ginseno side 성분을 분석한 결과, protopanaxadiol(PPD)계 ginsenoside인 Rb1, Rb2, Rc, Rd는 증숙 처리를 거치면서 함량이 감소한 반면 Rg3는 크게 증가하였다. 또한 protopanaxtriol(PPT)계 ginsenoside인 Re, Rg1은 구증구포 흑삼에서는거의 검출되지 않았으나, Rh1은 높은 함량을 나타내었다. 2. 백삼에서는 Benzo(a)pyrene 함량이 극히 미량이였으나, 증숙 횟수가 증가함에 따라 함량이 증가하였다. 98℃로 6시간씩 아홉 번 찌고 말린 흑삼의 경우 벤조피렌 함량이 0.19ppb로 식품의약품안전청 규정의 1/10 이하가 되어 안전한 것으로 평가 되었다. 3. 94℃로 3시간씩 아홉 번 증숙하여 건조시킨 흑삼을 10개월 동안 숙성 과정을 거쳐 Ginsenoside 함량을 분석한 결과, 숙성시키지 않은 백삼보다 Rg3, Rh1은 증가 하였으나, 98℃로 6시간씩 아홉 번 증숙하여 건조시킨 흑삼보다는 성분 증가가 높지 않았다. 94℃로 3시간씩 아홉 번 증숙하여 건조시킨 흑삼을 기준으로 5회~7회 증숙하여 말린 흑삼이 모든 성분을 충족시켜주는 것으로 나타났다. 4. 증숙 횟수에 따른 메탄올 추출물에 대한 인삼의 총 폴리페놀 함량은 4회 증숙부터 그 함량이 급격히 늘어났으며, 8회 증숙 시 가장 많은 폴리페놀 함량을 확인하였다. 5. 증숙 횟수에 따라 항산화활성을 분석한 결과, DPPH 라디칼 소거능은 9번 증숙처리 하였을 때의 활성이 증숙 하지 않은 백삼보다 약 10배 높은 항산화 활성을 보였다. 6. 증숙 횟수에 따른 흑삼의 항암활성을 분석한 결과, 인간 간암세포(HepG2)에서는 4번 증숙 시 높은 항암활성을 보였고, 1번 증숙한 인삼보다 더 높은 활성을 나타내었다.
흑삼 제조 과정에서 증숙 횟수 별, 온도, 시간차에 따른 성분 변화를 분석하여 흑삼 제조의 표준화를 규명하고, 항산화와 항암활성을 검정하여 약리효과에 대한 기초자료를 얻고자 수행 하였다. 1. 98℃로 6시간씩 아홉 번 증숙하여 건조시킨 흑삼의 조건별 Ginseno side 성분을 분석한 결과, protopanaxadiol(PPD)계 ginsenoside인 Rb1, Rb2, Rc, Rd는 증숙 처리를 거치면서 함량이 감소한 반면 Rg3는 크게 증가하였다. 또한 protopanaxtriol(PPT)계 ginsenoside인 Re, Rg1은 구증구포 흑삼에서는거의 검출되지 않았으나, Rh1은 높은 함량을 나타내었다. 2. 백삼에서는 Benzo(a)pyrene 함량이 극히 미량이였으나, 증숙 횟수가 증가함에 따라 함량이 증가하였다. 98℃로 6시간씩 아홉 번 찌고 말린 흑삼의 경우 벤조피렌 함량이 0.19ppb로 식품의약품안전청 규정의 1/10 이하가 되어 안전한 것으로 평가 되었다. 3. 94℃로 3시간씩 아홉 번 증숙하여 건조시킨 흑삼을 10개월 동안 숙성 과정을 거쳐 Ginsenoside 함량을 분석한 결과, 숙성시키지 않은 백삼보다 Rg3, Rh1은 증가 하였으나, 98℃로 6시간씩 아홉 번 증숙하여 건조시킨 흑삼보다는 성분 증가가 높지 않았다. 94℃로 3시간씩 아홉 번 증숙하여 건조시킨 흑삼을 기준으로 5회~7회 증숙하여 말린 흑삼이 모든 성분을 충족시켜주는 것으로 나타났다. 4. 증숙 횟수에 따른 메탄올 추출물에 대한 인삼의 총 폴리페놀 함량은 4회 증숙부터 그 함량이 급격히 늘어났으며, 8회 증숙 시 가장 많은 폴리페놀 함량을 확인하였다. 5. 증숙 횟수에 따라 항산화활성을 분석한 결과, DPPH 라디칼 소거능은 9번 증숙처리 하였을 때의 활성이 증숙 하지 않은 백삼보다 약 10배 높은 항산화 활성을 보였다. 6. 증숙 횟수에 따른 흑삼의 항암활성을 분석한 결과, 인간 간암세포(HepG2)에서는 4번 증숙 시 높은 항암활성을 보였고, 1번 증숙한 인삼보다 더 높은 활성을 나타내었다.
By analyzing component change according to streaming-drying cycle, temperature, time difference in the process of producing black ginseng, standardization in the production of black ginseng was investigated and by inspecting antioxidative and anticancer activities, fundamental data on medicinal effe...
By analyzing component change according to streaming-drying cycle, temperature, time difference in the process of producing black ginseng, standardization in the production of black ginseng was investigated and by inspecting antioxidative and anticancer activities, fundamental data on medicinal effect was acquired. 1. In the result of anlayzing Ginsenoside component by the condition of black ginseng which was steamed and dried 9 times for 6 hours at a time at 98℃, the contents of Rb1, Rb2, Rc, and Rd, which are ginsenoside in the family of protopanaxadiol(PPD), were reduced while passing through steaming-drying treatment but Rg3 increased greatly. Also, little Re and Rg1, which are ginsenoside in the family of protopanaxtriol(PPT), were found in the black ginseng but Rh1 showed high content. 2. In white ginseng, Benzo(a)pyrene content was very small but increased as the number of steaming-drying cycle increased. In the case of black ginseng which was steamed and dried 9 times for 6 hours at a time at 98℃, Benzo(a)pyrene content was 0.19ppb, which was under 1/10 of regulation of Food & Drug Administration, evaluated as safe. 3. In the result of analyzing Ginsenoside content by letting black ginseng, which was steamed and dried 9 times for 3 hours at a time at 94℃, pass through 10-month ripening process, Rg3 and Rh1 increased than not-ripened white ginseng but the increase of component was not high than black ginseng which was steamed and dried 9 times for 6 hours at a time at 98℃. Based on black ginseng which was streamed and dried 9 times for 3 hours at a time at 94℃, the black ginseng steamed and dried 5~7 times appeared satisfying all components. 4. Total polyphenol content of ginseng on methanol extract according to the number of steaming cycle increased rapidly after 4th steaming and 8th steaming showed the most polyphenol content. 5. In the result of analyzing antioxidant activity according to the number of steaming cycle, DPPH radical scavenging ability showed about 10 times higher antioxidant activity in the 9th steaming activity than no-steamed white ginseng. 6. In the result of analyzing anticancer activity of black ginseng according to the number of steaming cycle, human liver cells(HepG2) showed high anticancer activity at the 4th steaming and higher activity than the ginseng steamed once.
By analyzing component change according to streaming-drying cycle, temperature, time difference in the process of producing black ginseng, standardization in the production of black ginseng was investigated and by inspecting antioxidative and anticancer activities, fundamental data on medicinal effect was acquired. 1. In the result of anlayzing Ginsenoside component by the condition of black ginseng which was steamed and dried 9 times for 6 hours at a time at 98℃, the contents of Rb1, Rb2, Rc, and Rd, which are ginsenoside in the family of protopanaxadiol(PPD), were reduced while passing through steaming-drying treatment but Rg3 increased greatly. Also, little Re and Rg1, which are ginsenoside in the family of protopanaxtriol(PPT), were found in the black ginseng but Rh1 showed high content. 2. In white ginseng, Benzo(a)pyrene content was very small but increased as the number of steaming-drying cycle increased. In the case of black ginseng which was steamed and dried 9 times for 6 hours at a time at 98℃, Benzo(a)pyrene content was 0.19ppb, which was under 1/10 of regulation of Food & Drug Administration, evaluated as safe. 3. In the result of analyzing Ginsenoside content by letting black ginseng, which was steamed and dried 9 times for 3 hours at a time at 94℃, pass through 10-month ripening process, Rg3 and Rh1 increased than not-ripened white ginseng but the increase of component was not high than black ginseng which was steamed and dried 9 times for 6 hours at a time at 98℃. Based on black ginseng which was streamed and dried 9 times for 3 hours at a time at 94℃, the black ginseng steamed and dried 5~7 times appeared satisfying all components. 4. Total polyphenol content of ginseng on methanol extract according to the number of steaming cycle increased rapidly after 4th steaming and 8th steaming showed the most polyphenol content. 5. In the result of analyzing antioxidant activity according to the number of steaming cycle, DPPH radical scavenging ability showed about 10 times higher antioxidant activity in the 9th steaming activity than no-steamed white ginseng. 6. In the result of analyzing anticancer activity of black ginseng according to the number of steaming cycle, human liver cells(HepG2) showed high anticancer activity at the 4th steaming and higher activity than the ginseng steamed once.
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