To investigate anti-diabetic activities and atopy improvement effects of Opuntia humifusa cladodes, O. humifusa cladodes powder were extracted with 75% ethanol extract and fractionated with various solvents. Proximate composition and antioxidant activity of O. humifusa cladodes powder were estimated...
To investigate anti-diabetic activities and atopy improvement effects of Opuntia humifusa cladodes, O. humifusa cladodes powder were extracted with 75% ethanol extract and fractionated with various solvents. Proximate composition and antioxidant activity of O. humifusa cladodes powder were estimated. In addition, cookies and jelly added with the O. humifusa cladodes powder were prepared and their quality characteristics were evaluated. The results were as follows;
1. Moisture, crude protein, crude fat, and crude ash contents of O. humifusa cladodes were 3.15, 6.25, 2.12, and 11.35%, respectively. Hunter colors of O. humifusa cladodes showed L value 58.24, a value -10.44, and b value 20.42.
2. Dietary fiber contents were IDF 11.62%, SDF 33.04%, TDF 44.66%, respectively. Major free sugars of O. humifusa cladodes were detected as fructose, glucose, and sucrose, sucrose content was the highest 2,956.65±57.35 mg%. Major organic acids were oxalic acid, citric acid, tartaric acid, malic acid, and acetic acid. Mineral were detected as Fe, K, Mg, Ca, Mn, Cu, Zn, and Na. Among them, Ca content was the highest 40,470.00±5,190.16 mg/kg. Free amino acid showed relatively low content.
3. α-Amylase inhibitory activity of 75% ethanol extract of O. humifusa cladodes showed the highest 35.98±5.27%. In addition, α-glucosidase inhibitory activity of 75% ethanol extract was the highest 23.48±6.81%.
4. Handscopic feature of powder-free basal diet, control group was observed more serious skin lesions (erythema/hemorrhage, scarring/dryness, edema, excoriation/erosion) than those (scarring/dryness, edema) of 10% water extract (OHW) and 10% ethanol extract (OHE) of O. humifusa cladodes.
5. Distribution of mast cells in dermis and subcutaneous tissue of skin was more decreased in skin of OHE groups than those of control group and OHW group.
6. Mast cell tryptase and myeloperoxidase immune-reactive cells were more decreased in skin of OHW and OHE groups than that of control group.
7. Serum IgE contents of OHW group were more increased 1,446.6±12.9 ng/mL than that of the control group, but that of OHE group decreased than that of the control group.
8. Serum histamine contents of OHW and OHE groups were more decreased 123.9±34.9 and 128.4±4.3 nM, respectively, than that of the control group.
9. Serum IL-4 contents of OHW and OHE groups were more increased 81.4±2.7 and 118.8±2.9 pg/mL, respectively, than that of the control group. Serum IL-10 contents of OHW groups showed 40.8±0.3 pg/mL, but that of OHE group was higher 84.1±3.7 pg/mL than that of the control group.
10. α-Amylase and α-glucosidase inhibitory activity of ethylacetate fraction were the highest 89.94±1.15 and 29.01±3.03%, respectively.
11. Total phenolics and flavonoids contents of ethylacetate fraction showed the highest.
12. DPPH and ABTS radical activities of O. humifusa cladodes extracts were increased according to concentration and those of ethylacetate fraction was the highest. Ferric reducing antioxidant power of chloroform fraction was the highest.
13. Overall acceptability score of cookie added with 5% of the O. humifusa cladodes powder was the highest 7.47±1.51 with significant difference.
14. Overall acceptability score of jelly added with 5% of the O. humifusa cladodes powder was the highest 6.47±1.96 with no significant difference.
15. Moisture, crude protein, crude fat, crude ash, and soluble solid contents of cookie added with 5% of O. humifusa cladodes powder were 4.67±0.08%, 7.45±0.06%, 18.30±0.11%, 1.26±0.10%, and 2.77±0.06˚Brix, respectively. Mineral contents of cookie were detected as K, Mg, Ca, and Na. Ca content of cookie was the highest 2,212.33±83.92 mg/kg. Dietary fiber contents were IDF 8.15%, SDF 0.16%, and TDF 8.30%. Hardness of cookie was 3,271.60±373.98 g.
16. Moisture, crude protein, crude fat, crude ash, and soluble solid contents of jelly added with 5% of O. humifusa cladodes powder were 442.85±1.41%, 2.77±0.07%, 0.27±0.05%, 0.31±0.17%, and 1.70±0.00˚Brix, respectively. Ca content of jelly was the highest 1,220.43±208.64 mg/kg. Dietary fiber contents were IDF 0.90±0.36%, SDF 1.16±0.80%, TDF 2.07±0.61%.
In conclusion, we confirmed anti-diabetic activities and atopy improvement effects of O. humifusa cladodes. Therefore, we confirmed that the powder of O. humifusa cladodes seemed to have potential as a useful ingredient to prepare foods including cookie and jelly.
To investigate anti-diabetic activities and atopy improvement effects of Opuntia humifusa cladodes, O. humifusa cladodes powder were extracted with 75% ethanol extract and fractionated with various solvents. Proximate composition and antioxidant activity of O. humifusa cladodes powder were estimated. In addition, cookies and jelly added with the O. humifusa cladodes powder were prepared and their quality characteristics were evaluated. The results were as follows;
1. Moisture, crude protein, crude fat, and crude ash contents of O. humifusa cladodes were 3.15, 6.25, 2.12, and 11.35%, respectively. Hunter colors of O. humifusa cladodes showed L value 58.24, a value -10.44, and b value 20.42.
2. Dietary fiber contents were IDF 11.62%, SDF 33.04%, TDF 44.66%, respectively. Major free sugars of O. humifusa cladodes were detected as fructose, glucose, and sucrose, sucrose content was the highest 2,956.65±57.35 mg%. Major organic acids were oxalic acid, citric acid, tartaric acid, malic acid, and acetic acid. Mineral were detected as Fe, K, Mg, Ca, Mn, Cu, Zn, and Na. Among them, Ca content was the highest 40,470.00±5,190.16 mg/kg. Free amino acid showed relatively low content.
3. α-Amylase inhibitory activity of 75% ethanol extract of O. humifusa cladodes showed the highest 35.98±5.27%. In addition, α-glucosidase inhibitory activity of 75% ethanol extract was the highest 23.48±6.81%.
4. Handscopic feature of powder-free basal diet, control group was observed more serious skin lesions (erythema/hemorrhage, scarring/dryness, edema, excoriation/erosion) than those (scarring/dryness, edema) of 10% water extract (OHW) and 10% ethanol extract (OHE) of O. humifusa cladodes.
5. Distribution of mast cells in dermis and subcutaneous tissue of skin was more decreased in skin of OHE groups than those of control group and OHW group.
6. Mast cell tryptase and myeloperoxidase immune-reactive cells were more decreased in skin of OHW and OHE groups than that of control group.
7. Serum IgE contents of OHW group were more increased 1,446.6±12.9 ng/mL than that of the control group, but that of OHE group decreased than that of the control group.
8. Serum histamine contents of OHW and OHE groups were more decreased 123.9±34.9 and 128.4±4.3 nM, respectively, than that of the control group.
9. Serum IL-4 contents of OHW and OHE groups were more increased 81.4±2.7 and 118.8±2.9 pg/mL, respectively, than that of the control group. Serum IL-10 contents of OHW groups showed 40.8±0.3 pg/mL, but that of OHE group was higher 84.1±3.7 pg/mL than that of the control group.
10. α-Amylase and α-glucosidase inhibitory activity of ethylacetate fraction were the highest 89.94±1.15 and 29.01±3.03%, respectively.
11. Total phenolics and flavonoids contents of ethylacetate fraction showed the highest.
12. DPPH and ABTS radical activities of O. humifusa cladodes extracts were increased according to concentration and those of ethylacetate fraction was the highest. Ferric reducing antioxidant power of chloroform fraction was the highest.
13. Overall acceptability score of cookie added with 5% of the O. humifusa cladodes powder was the highest 7.47±1.51 with significant difference.
14. Overall acceptability score of jelly added with 5% of the O. humifusa cladodes powder was the highest 6.47±1.96 with no significant difference.
15. Moisture, crude protein, crude fat, crude ash, and soluble solid contents of cookie added with 5% of O. humifusa cladodes powder were 4.67±0.08%, 7.45±0.06%, 18.30±0.11%, 1.26±0.10%, and 2.77±0.06˚Brix, respectively. Mineral contents of cookie were detected as K, Mg, Ca, and Na. Ca content of cookie was the highest 2,212.33±83.92 mg/kg. Dietary fiber contents were IDF 8.15%, SDF 0.16%, and TDF 8.30%. Hardness of cookie was 3,271.60±373.98 g.
16. Moisture, crude protein, crude fat, crude ash, and soluble solid contents of jelly added with 5% of O. humifusa cladodes powder were 442.85±1.41%, 2.77±0.07%, 0.27±0.05%, 0.31±0.17%, and 1.70±0.00˚Brix, respectively. Ca content of jelly was the highest 1,220.43±208.64 mg/kg. Dietary fiber contents were IDF 0.90±0.36%, SDF 1.16±0.80%, TDF 2.07±0.61%.
In conclusion, we confirmed anti-diabetic activities and atopy improvement effects of O. humifusa cladodes. Therefore, we confirmed that the powder of O. humifusa cladodes seemed to have potential as a useful ingredient to prepare foods including cookie and jelly.
Keyword
#천년초
#항당뇨
#아토피
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