Objectives : Obesity-induced inflammation, which is characterized by enhanced production of various inflammatory cytokines and activation of inflammatory signaling networks, contributes to the development of obesity-related complications such as insulin resistance, type II diabetes, and cardiovascul...
Objectives : Obesity-induced inflammation, which is characterized by enhanced production of various inflammatory cytokines and activation of inflammatory signaling networks, contributes to the development of obesity-related complications such as insulin resistance, type II diabetes, and cardiovascular disease. Dysregulation of obesity-induced inflammation production plays a crucial role in the obesity, and thus targeting obesity-induced inflammation may be a useful strategy to prevent or attenuate the obesity and-related pathologies. The objective of this study was to investigate the effect of Commiphora abyssinica (CA) 80% ethanol extract on adipocyte differentiation and adipogenesis in 3T3-L1 cell. Thereafter the obesity-induced inflammation investigate the effects of anti-obesity factors in RAW 264.7 cell. Methods : CA was prepared by extracting with 80% ethanol. Cell viability was assessed by MTT assay using RAW 264.7 cells and 3T3-L1 cells. Anti-obesity activity was measured in lipid droplets and triglyceride (TG) accumulation in 3T3-L1 cells. We also analyzed the expression of C/EBPβ, C/EBPα, PPARγ, SREBP1c, and aP2 by RT-PCR. In addition, we observed the production of fatty acid, acetyl-CoA carboxylase and Oil-red O staining. Obesity-induced-inflammation activities of each extract were measured through changes in the levels of Reactive oxygen species (ROS), nitric oxide (NO), prostaglandin E2 (PGE2), and inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α on RAW 264.7 cell. Results : No cytotoxicity from CA 80% ethanol extracts was observed at the concentration of 1, 10, 100 (㎍/㎖) in 3T3-L1 and RAW 264.7 cells. Treatment with CA significantly suppressed the terminal differentiation of 3T3-L1 in a dose-dependent manner, as confirmed by a decrease in triglyceride and Fatty acid and Acetyl-CoA carboxylase. Also, CA exhibited potential adipogenesis inhibition and downregulated the expression of pro-adipogenic transcription factors, such as sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα) and C/EBPβ, and adipocyte expressed genes, such as adipocyte fatty acid binding protein (aP2) and Fas. In addition, lipid accumulation determined by Oil-red O staining showed that CA extract had inhibitory effects on lipid accumulation in 3T3-L1 cells. Obesity-induced-inflammation response of RAW 264.7 cells was induced by lipopolysaccharide (LPS), followed by each CA treatment at indicated concentrations (1, 10, 100 ㎍/㎖). CA showed the highest anti-oxidant activities and decrease obesity-induced-inflammation factors on LPS-induced RAW 264.7 cells. Conclusion : These results suggest that CA suppresses obesity factors by modulating obesity-induced inflammation release from 3T3-L1 and RAW 264.7 cells. CA may be a useful medical herbs for attenuating obesity-induced inflammation and metabolic diseases such as obesity.
Objectives : Obesity-induced inflammation, which is characterized by enhanced production of various inflammatory cytokines and activation of inflammatory signaling networks, contributes to the development of obesity-related complications such as insulin resistance, type II diabetes, and cardiovascular disease. Dysregulation of obesity-induced inflammation production plays a crucial role in the obesity, and thus targeting obesity-induced inflammation may be a useful strategy to prevent or attenuate the obesity and-related pathologies. The objective of this study was to investigate the effect of Commiphora abyssinica (CA) 80% ethanol extract on adipocyte differentiation and adipogenesis in 3T3-L1 cell. Thereafter the obesity-induced inflammation investigate the effects of anti-obesity factors in RAW 264.7 cell. Methods : CA was prepared by extracting with 80% ethanol. Cell viability was assessed by MTT assay using RAW 264.7 cells and 3T3-L1 cells. Anti-obesity activity was measured in lipid droplets and triglyceride (TG) accumulation in 3T3-L1 cells. We also analyzed the expression of C/EBPβ, C/EBPα, PPARγ, SREBP1c, and aP2 by RT-PCR. In addition, we observed the production of fatty acid, acetyl-CoA carboxylase and Oil-red O staining. Obesity-induced-inflammation activities of each extract were measured through changes in the levels of Reactive oxygen species (ROS), nitric oxide (NO), prostaglandin E2 (PGE2), and inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α on RAW 264.7 cell. Results : No cytotoxicity from CA 80% ethanol extracts was observed at the concentration of 1, 10, 100 (㎍/㎖) in 3T3-L1 and RAW 264.7 cells. Treatment with CA significantly suppressed the terminal differentiation of 3T3-L1 in a dose-dependent manner, as confirmed by a decrease in triglyceride and Fatty acid and Acetyl-CoA carboxylase. Also, CA exhibited potential adipogenesis inhibition and downregulated the expression of pro-adipogenic transcription factors, such as sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα) and C/EBPβ, and adipocyte expressed genes, such as adipocyte fatty acid binding protein (aP2) and Fas. In addition, lipid accumulation determined by Oil-red O staining showed that CA extract had inhibitory effects on lipid accumulation in 3T3-L1 cells. Obesity-induced-inflammation response of RAW 264.7 cells was induced by lipopolysaccharide (LPS), followed by each CA treatment at indicated concentrations (1, 10, 100 ㎍/㎖). CA showed the highest anti-oxidant activities and decrease obesity-induced-inflammation factors on LPS-induced RAW 264.7 cells. Conclusion : These results suggest that CA suppresses obesity factors by modulating obesity-induced inflammation release from 3T3-L1 and RAW 264.7 cells. CA may be a useful medical herbs for attenuating obesity-induced inflammation and metabolic diseases such as obesity.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.