밀싹 추출물에 대하여 생리활성 및 화장품 소재로서의 가능성과 산업적 활용가치를 확인하고자 항산화, 항염, 멜라닌 생합성 억제능, MMP-1 발현 억제, 피부장벽 회복에 미치는 영향에 대하여 확인하고자 하였다. 먼저 상온수, 열수 그리고 50% 에탄올을 이용하는 3가지 방법으로 밀 씨앗, 밀싹 추출물을 획득하고 이들에 대한 항산화력을 비교한 결과 50% 에탄올을 이용한 밀싹 추출물에서 가장 높은 항산화력이 확인되어 50% 에탄올을 이용한 밀싹 추출물을 화장품 소재로서의 사용 가능성을 확인하였다. 밀싹 추출물이 고온 보존이 항산화력에 미치는 영향을 조사한 결과 고온 보존이 기간이 증가할수록 밀싹 추출물의 항산화력이 감소하는 것을 확인하였다. 밀싹 추출물을 이용한 세포 실험은 진행한 결과 밀싹 추출물의 RAW 264.7, B16F10, HDF 모든 세포에서 유의한 세포독성이 나타나지 않았다. ...
밀싹 추출물에 대하여 생리활성 및 화장품 소재로서의 가능성과 산업적 활용가치를 확인하고자 항산화, 항염, 멜라닌 생합성 억제능, MMP-1 발현 억제, 피부장벽 회복에 미치는 영향에 대하여 확인하고자 하였다. 먼저 상온수, 열수 그리고 50% 에탄올을 이용하는 3가지 방법으로 밀 씨앗, 밀싹 추출물을 획득하고 이들에 대한 항산화력을 비교한 결과 50% 에탄올을 이용한 밀싹 추출물에서 가장 높은 항산화력이 확인되어 50% 에탄올을 이용한 밀싹 추출물을 화장품 소재로서의 사용 가능성을 확인하였다. 밀싹 추출물이 고온 보존이 항산화력에 미치는 영향을 조사한 결과 고온 보존이 기간이 증가할수록 밀싹 추출물의 항산화력이 감소하는 것을 확인하였다. 밀싹 추출물을 이용한 세포 실험은 진행한 결과 밀싹 추출물의 RAW 264.7, B16F10, HDF 모든 세포에서 유의한 세포독성이 나타나지 않았다. RAW 264.7 세포에 대한 밀싹 추출물의 항염 효과를 확인한 결과, 저농도에서도 뛰어난 NO 생성이 억제와 COX-2 유전자 발현을 효과적으로 억제시키고, iNOS의 유전자 발현이 감소하는 것으로 확인되었다. 멜라닌 생성 저해능 실험 결과 멜라닌 생합성 억제효과는 다소 약하게 나타났다. MMP-1 발현에 미치는 영향에 대해 실험한 결과, 밀싹 추출물이 MMP-1 발현이 억제되는 것을 확인할 수 있었으며, p-ERK 신호전달 경로를 통하여 MMP-1 발현이 억제되는 것을 확인하였다. 밀싹 추출물을 포함한 화장품을 제조한 후 피부의 장벽 회복 속도를 측정 및 관찰한 결과 밀싹을 함유한 크림은 일반 크림보다 경피 수분 손실량을 효과적으로 줄여주어 피부장벽 기능에 도움을 주는 것을 확인할 수 있었다. 따라서 본 연구는 밀싹 추출물의 기능성 화장품 소재로 사용 시 항산화, 피부 염증 예방과 피부 노화 예방 관점에서의 기능성 화장품 소재로서 사용하는 것을 제안하며 향후 임상적 활용 가능성을 판단하기 위하여 동물, 인체 시험을 통한 밀싹 추출물의 효능 검증 연구가 체계적으로 진행되어야 할 것이다.
밀싹 추출물에 대하여 생리활성 및 화장품 소재로서의 가능성과 산업적 활용가치를 확인하고자 항산화, 항염, 멜라닌 생합성 억제능, MMP-1 발현 억제, 피부장벽 회복에 미치는 영향에 대하여 확인하고자 하였다. 먼저 상온수, 열수 그리고 50% 에탄올을 이용하는 3가지 방법으로 밀 씨앗, 밀싹 추출물을 획득하고 이들에 대한 항산화력을 비교한 결과 50% 에탄올을 이용한 밀싹 추출물에서 가장 높은 항산화력이 확인되어 50% 에탄올을 이용한 밀싹 추출물을 화장품 소재로서의 사용 가능성을 확인하였다. 밀싹 추출물이 고온 보존이 항산화력에 미치는 영향을 조사한 결과 고온 보존이 기간이 증가할수록 밀싹 추출물의 항산화력이 감소하는 것을 확인하였다. 밀싹 추출물을 이용한 세포 실험은 진행한 결과 밀싹 추출물의 RAW 264.7, B16F10, HDF 모든 세포에서 유의한 세포독성이 나타나지 않았다. RAW 264.7 세포에 대한 밀싹 추출물의 항염 효과를 확인한 결과, 저농도에서도 뛰어난 NO 생성이 억제와 COX-2 유전자 발현을 효과적으로 억제시키고, iNOS의 유전자 발현이 감소하는 것으로 확인되었다. 멜라닌 생성 저해능 실험 결과 멜라닌 생합성 억제효과는 다소 약하게 나타났다. MMP-1 발현에 미치는 영향에 대해 실험한 결과, 밀싹 추출물이 MMP-1 발현이 억제되는 것을 확인할 수 있었으며, p-ERK 신호전달 경로를 통하여 MMP-1 발현이 억제되는 것을 확인하였다. 밀싹 추출물을 포함한 화장품을 제조한 후 피부의 장벽 회복 속도를 측정 및 관찰한 결과 밀싹을 함유한 크림은 일반 크림보다 경피 수분 손실량을 효과적으로 줄여주어 피부장벽 기능에 도움을 주는 것을 확인할 수 있었다. 따라서 본 연구는 밀싹 추출물의 기능성 화장품 소재로 사용 시 항산화, 피부 염증 예방과 피부 노화 예방 관점에서의 기능성 화장품 소재로서 사용하는 것을 제안하며 향후 임상적 활용 가능성을 판단하기 위하여 동물, 인체 시험을 통한 밀싹 추출물의 효능 검증 연구가 체계적으로 진행되어야 할 것이다.
Possibility and industrial usefulness of wheat sprout extract as functional cosmetics material was to be confirmed. First of all, three methods that use cold water, hot channel, and 50% ethanol were used to acquire wheat seed and extract, and as a result of comparing their antioxidative activity, wh...
Possibility and industrial usefulness of wheat sprout extract as functional cosmetics material was to be confirmed. First of all, three methods that use cold water, hot channel, and 50% ethanol were used to acquire wheat seed and extract, and as a result of comparing their antioxidative activity, wheat sprout extract had the highest antioxidative activity. Usability as cosmetics material by using 50% ethanol wheat sprout extract, which had the most excellent antioxidative activity in the first experiment result, was confirmed. As a result of investigating the effect of high temperature storage on the antioxidative activity of wheat sprout extract extracted with 50% ethanol, it was confirmed that the antioxidative activity of wheat sprout extract decreased as the period of high temperature storage increased. As a result of conducting cell experiment using wheat sprout extract extracted using 50% ethanol, all cells of RAW 264.7, B16F10, HDF of wheat sprout extract displayed no significant cytotoxicity. In order to observe antiinflammatory effect of RAW 264.7 cell, when wheat sprout extract was treated by concentration, it was possible to confirm that NO formation, which is outstanding even in low concentration, was being restrained compared to LPS treatment group. When expression ratio of COX-2 preprocessed wheat sprout extract, it effectively restrained the manifestation of COX-2 gene, which is the infection spreading factor, within cell compared to control, and as a result of experimenting gene manifestation of inflammatory cytokine of wheat sprout extract, it was confirmed that gene manifestation of iNOS decreased. As a result of melanin formation inhibitory activity experiment, melanin biosynthetic controlling effect appeared rather weak, since there wasn't much difference between both wheat sprout extract treatment by concentration and exclusive a-MSH treatment in B16F10 melanoma. As a result of experimenting the influence on MMP-1 manifestation, it was possible to confirm MMP-1 manifestation restraint of wheat sprout extract, and as a result of confirming the phosphorylation change of p-JNK and p-ERK, which are known to be related with MMP-1 manifestation, it was possible to confirm that wheat sprout extract was restrained of MMP-1 manifestation through p-ERK signalling pathway in low concentration. After producing cosmetics that include wheat sprout extract, clinical subjects had their inner arm lose skin moisture and wall function damaged for in vivo clinical test, and cosmetics were divided into cosmetics with wheat sprout extract and control group. As a result of measuring and observing the regeneration speed of damaged skin using percutaneous moisture loss measuring instrument, it was possible to confirm that the cream with wheat sprout effectively recovered the function of damaged skin wall compared to normal cream, and aided the function of skin wall by effectively reducing percutaneous moisture loss. With the above results, this research was able to assume that wheat sprout extract had little toxicity against cells, and its effect of antiinflammatory by participating in the restraint of NO manifestation by restraining gene manifestation of COX-2 and iNOS, induced by LPS. Also, by hindering the manifestation of MMP-1 induced by polyphenol, high content of flavonoid, and UV rays and the result of restraining photoaging of skin, it was possible to confirm its effect on preventing skin wrinkles, caused by photoaging and natural aging. Therefore, this research proposes using wheat sprout as functional cosmetics for its antioxidative activity, skin inflammation prevention, and skin aging prevention when using it as functional cosmetics material, and in order to judge its future clinical usability, systematic research should be conducted in order to verify its effects through animal and human trials.
Possibility and industrial usefulness of wheat sprout extract as functional cosmetics material was to be confirmed. First of all, three methods that use cold water, hot channel, and 50% ethanol were used to acquire wheat seed and extract, and as a result of comparing their antioxidative activity, wheat sprout extract had the highest antioxidative activity. Usability as cosmetics material by using 50% ethanol wheat sprout extract, which had the most excellent antioxidative activity in the first experiment result, was confirmed. As a result of investigating the effect of high temperature storage on the antioxidative activity of wheat sprout extract extracted with 50% ethanol, it was confirmed that the antioxidative activity of wheat sprout extract decreased as the period of high temperature storage increased. As a result of conducting cell experiment using wheat sprout extract extracted using 50% ethanol, all cells of RAW 264.7, B16F10, HDF of wheat sprout extract displayed no significant cytotoxicity. In order to observe antiinflammatory effect of RAW 264.7 cell, when wheat sprout extract was treated by concentration, it was possible to confirm that NO formation, which is outstanding even in low concentration, was being restrained compared to LPS treatment group. When expression ratio of COX-2 preprocessed wheat sprout extract, it effectively restrained the manifestation of COX-2 gene, which is the infection spreading factor, within cell compared to control, and as a result of experimenting gene manifestation of inflammatory cytokine of wheat sprout extract, it was confirmed that gene manifestation of iNOS decreased. As a result of melanin formation inhibitory activity experiment, melanin biosynthetic controlling effect appeared rather weak, since there wasn't much difference between both wheat sprout extract treatment by concentration and exclusive a-MSH treatment in B16F10 melanoma. As a result of experimenting the influence on MMP-1 manifestation, it was possible to confirm MMP-1 manifestation restraint of wheat sprout extract, and as a result of confirming the phosphorylation change of p-JNK and p-ERK, which are known to be related with MMP-1 manifestation, it was possible to confirm that wheat sprout extract was restrained of MMP-1 manifestation through p-ERK signalling pathway in low concentration. After producing cosmetics that include wheat sprout extract, clinical subjects had their inner arm lose skin moisture and wall function damaged for in vivo clinical test, and cosmetics were divided into cosmetics with wheat sprout extract and control group. As a result of measuring and observing the regeneration speed of damaged skin using percutaneous moisture loss measuring instrument, it was possible to confirm that the cream with wheat sprout effectively recovered the function of damaged skin wall compared to normal cream, and aided the function of skin wall by effectively reducing percutaneous moisture loss. With the above results, this research was able to assume that wheat sprout extract had little toxicity against cells, and its effect of antiinflammatory by participating in the restraint of NO manifestation by restraining gene manifestation of COX-2 and iNOS, induced by LPS. Also, by hindering the manifestation of MMP-1 induced by polyphenol, high content of flavonoid, and UV rays and the result of restraining photoaging of skin, it was possible to confirm its effect on preventing skin wrinkles, caused by photoaging and natural aging. Therefore, this research proposes using wheat sprout as functional cosmetics for its antioxidative activity, skin inflammation prevention, and skin aging prevention when using it as functional cosmetics material, and in order to judge its future clinical usability, systematic research should be conducted in order to verify its effects through animal and human trials.
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