[논문]화장품 소재로서 아로니아 발효추출물의 생리활성 연구

강정란; 오동순; 황소소; 김종화; 한갑훈

doi:10.22156/cs4smb.2020.10.01.243

화장품 소재로서 아로니아 발효추출물의 생리활성 연구
A Study of Physiological Activities for Cosmeceutical Ingredient from Fermented Aroniamelanocarpa Extract 원문보기

융합정보논문지 = Journal of Convergence for Information Technology, v.10 no.1, 2020년, pp.243 - 250

강정란 (우석대학교 제약공학과) , 오동순 (우석대학교 제약공학과) , 황소소 (우석대학교 일반대학원 제약.화장품 공학과) , 김종화 (우석대학교 제약공학과) , 한갑훈 (우석대학교 제약공학과)

초록
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아로니아는 특유의 신맛과 떫은 맛 때문에 생과보다는 가공용으로 개발할 필요성이 대두되고 있다. 유산균을 이용한 아로니아분말 발효추출물에 대한 항산화 활성 및 세포독성을 검정한 결과 총 폴리페놀 함량의 경우 발효전 32.15 ㎍/mg, 발효 후 43.08 ㎍/mg으로 증가하였고, 플라보노이드 함량은 발효전 0.47 ㎍/mg, 0.44 ㎍/mg 으로 조금 높거나 유사하게 나타내었다. DPPH radical 억제 활성은 발효전 77.5% 에서 89.1%로 발효전보다 조금더 높은 활성을 나타냈으며, Nitric oxide (NO) 측정은 농도 의존적인 억제효능이 나타났다. 이상의 결과로부터 아로니아분말을 발효할 경우 일부 항산화 활성을 기반으로한 활용가능성과 향후 식물성 천연 추출물과 발효 화장품 소재로서의 이용가능성을 확인할 수 있었다.

Abstract ▼ AI-Helper

Due to the unique sourness and sweet taste of Aronia, it is necessary to develop it for processing rather than raw. The antioxidant activity and cytotoxin about the aronia powder fermentation extract using lactobacillus are verified. In the case of total polyphenol content, the content of non-fermented extract was 32.15 ㎍/mg fermentation extract, 43.08 ㎍/mg after fermentation, and the flavonoid content was 0.47 ㎍/mg in non-fermented extract and 0.44 ㎍/mg in fermented extract, which was similar to that of non-fermented extract. In the DPPH radical inhibition assay, 77.5% of the non-fermented extract and 89.1% of the fermented extract showed better activity than the non-fermented extract. Nitric oxide (NO) measurement showed a concentration-dependent inhibitory effect. From the above results, it was confirmed that the fermentation of Aronia powder could be utilized based on some antioxidant activities and the possibility of using it as a vegetable extract and fermented cosmetic material in the future.

주제어

표/그림 (5)

그림 Fig. 1. DPPH Radical Scavenging Activity of non-fermented and fermented Aroniamelanocarpa Extract.
그림 Fig. 2. Total polyphenol contents in non-fermentes and fermented Aroniamelanocarpa Extract.
그림 Fig. 3. Total flavonoid contents in non-fermentes and fermented Aroniamelanocarpa Extract.
그림 Fig. 4. Percentage of fibroblast cell viability at various concentration of Fermented Aroniamelanocarpa Extract.
그림 Fig. 5. Percentage of NO production at a various cultural concentration of Fermented Aroniamelanocarpa Extract.

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문제 정의

So far, researches on the main function and product development of cosmetic composition for Aronia extract have been going on, but research data on the physiological activity and efficacy of Aronia fermented extract as a cosmetic material are not yet available. The purpose of this study was to examine the feasibility of using the Aronia fermented extract as a cosmetic material. Therefore, we intend to provide the basic data on the vitalization of research on the natural extracts of plant and fermented cosmetic materials.

대상 데이터

RAW264.7 (TIB-71; ATCC, USA) macrophages were purchased from American type cell collection (ATCC, USA), the medium used was prepared by adding 10% FBS (Fetal Bovine Serum; Gibco, USA) and 1% Penicillin (100 U / mL) / Streptocycin (100 μg / mL) to Dulbecco's Modified Eagle's Medium After sufficient proliferation in 75cm2 flask (SPL, Korea), the cells were washed three times with phosphate buffered saline solution at 3-day intervals, and trypsin solution was added.
The Aronia cultivar used in this experiment is the national seed source application number 2015-399 of the black choke berry variety registered in the national seed source, and it is produced in Jinan County agricultural products processing center in February, Highland aronia fruit powder (100%) was purchased and used. The composition of the fermentation medium was sterilized at 121°C and 1.

이론/모형

DPPH radical scavenging activity was measured by changing the Blois MS method [10] to determine the electron donating ability by 0.1 mM DPPH (2,2-diphenyl-1-picrylhydrazyl, Sigma Chemical Co., St. Louis, donating ability, EDA) were measured. That is, the solution was added to a 96-well plate in an amount equivalent to 180 μl, and 20 μL of the sample dissolved in the second distilled water was added to the well containing 0.

성능/효과

1% concentration, and concentration-dependent inhibitory effect was shown. From the above results, it was found that the fermentation of the Aronia powder increased the antioxidative activity and the radical scavenging ability, and it could be confirmed that it could be used as a plant natural extract and a fermented cosmetic material.
1% to less than 50%. It was confirmed that the fermented Ahnonia fermented extracts through yeast and lactic acid bacteria had an inhibitory effect on NO production.

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