Abstract To evaluate the antioxidant activity of clove essential oil, DPPH & ABTS radical scavenging activity and ROS production were measured. To prove the antiinflammatory effect, we measured the production and mRNA expression of NO, PGE2, IL-1β, IL-6 and TNF-α in Raw264.7 cells and ICR mice. And ...
Abstract To evaluate the antioxidant activity of clove essential oil, DPPH & ABTS radical scavenging activity and ROS production were measured. To prove the antiinflammatory effect, we measured the production and mRNA expression of NO, PGE2, IL-1β, IL-6 and TNF-α in Raw264.7 cells and ICR mice. And also, a histological change in the back, ears and liver tissue in Raw 264.7 cell and ICR mouse animal model was observed. The experimental results are as follows. 1. Clove oil was indicated to have DPPH radical & ABTS radical scavenging activity and to have very high radical scavenging activity compared to BHA. However, inhibitory activity of ROS production wasn't shown. 2. Clove oil could be known to have antiinflammatory activity in LPS-activated Raw 264.7 cells by reducing NO (p<0.05), PGE2 (p<0.001), IL-1β (p<0.01), IL-6 (p<0.01) and TNF-α production (p<0.001). 3. Clove oil could be known to have anti-inflammatory activity in LPS-activated ICR mouse serum by decreasing NO (p<0.05), PGE2 (p<0.05), IL-6 (p<0.05) and TNF-α production(p<0.05). However, IL-1β production in the ICR mouse serum was slightly reduced compared to a negative control group. 4. Clove oil could be known to have anti-inflammatory activity in LPS-activated Raw 264.7 cells by reducing IL-1β (p<0.001), IL-6 (p<0.001) and TNF-α expression (p<0.001). 5. Clove oil could be known to have anti-inflammatory activity in LPS-activated ICR mouse serum by making it available for decreasing IL-6 (p<0.001) and TNF-α expression (p<0.001). However, IL-1β expression in the ICR mouse serum was slightly reduced compared to a negative control group. 6. In histological examination of the back, ears and liver tissue in the LPS-induced ICR mouse, it could be confirmed to be relieved inflammation and edema in a group of clove oil treatment. In consequence of this study, the antioxidant and antiinflammatory activity in clove oil were verified significantly. Through a systematic and in-depth research on clove oil based on a previous research and the outcome in this study, diverse drugs and cosmetic products can be developed and applied to daily life and a clinical field.
Abstract To evaluate the antioxidant activity of clove essential oil, DPPH & ABTS radical scavenging activity and ROS production were measured. To prove the antiinflammatory effect, we measured the production and mRNA expression of NO, PGE2, IL-1β, IL-6 and TNF-α in Raw264.7 cells and ICR mice. And also, a histological change in the back, ears and liver tissue in Raw 264.7 cell and ICR mouse animal model was observed. The experimental results are as follows. 1. Clove oil was indicated to have DPPH radical & ABTS radical scavenging activity and to have very high radical scavenging activity compared to BHA. However, inhibitory activity of ROS production wasn't shown. 2. Clove oil could be known to have antiinflammatory activity in LPS-activated Raw 264.7 cells by reducing NO (p<0.05), PGE2 (p<0.001), IL-1β (p<0.01), IL-6 (p<0.01) and TNF-α production (p<0.001). 3. Clove oil could be known to have anti-inflammatory activity in LPS-activated ICR mouse serum by decreasing NO (p<0.05), PGE2 (p<0.05), IL-6 (p<0.05) and TNF-α production(p<0.05). However, IL-1β production in the ICR mouse serum was slightly reduced compared to a negative control group. 4. Clove oil could be known to have anti-inflammatory activity in LPS-activated Raw 264.7 cells by reducing IL-1β (p<0.001), IL-6 (p<0.001) and TNF-α expression (p<0.001). 5. Clove oil could be known to have anti-inflammatory activity in LPS-activated ICR mouse serum by making it available for decreasing IL-6 (p<0.001) and TNF-α expression (p<0.001). However, IL-1β expression in the ICR mouse serum was slightly reduced compared to a negative control group. 6. In histological examination of the back, ears and liver tissue in the LPS-induced ICR mouse, it could be confirmed to be relieved inflammation and edema in a group of clove oil treatment. In consequence of this study, the antioxidant and antiinflammatory activity in clove oil were verified significantly. Through a systematic and in-depth research on clove oil based on a previous research and the outcome in this study, diverse drugs and cosmetic products can be developed and applied to daily life and a clinical field.
주제어
#free radical clove oil antioxidation antiinflammation
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