Magnetic nanoparticles have been used in biochemical and nucleic acid separation and detection, and as magnetic resonance imaging contrast agents in the diagnostic field. Meanwhile, in immunochromatographic assays, gold (Au) nanoparticles are widely used as signal materials. Gold nanoparticles have ...
Magnetic nanoparticles have been used in biochemical and nucleic acid separation and detection, and as magnetic resonance imaging contrast agents in the diagnostic field. Meanwhile, in immunochromatographic assays, gold (Au) nanoparticles are widely used as signal materials. Gold nanoparticles have highly negatively charged surfaces, and therefore, can be conjugated with protein through electrostatic interactions. However, because of their negatively charged surfaces, if the proteins used for labeling are stored in high-salt concentration buffers, gold nanoparticles can be aggregated. Importantly, magnetic nanoparticles have an advantage of being inexpensive compared with gold nanoparticles. Using magnetic nanoparticles, biomolecules can be easily labeled as carboxylated magnetic nanoparticles and, thus, be easily separated by a permanent magnet. In this study, an immunochromatographic assay for the detection of hepatitis B surface antigen (HBsAg) was developed using magnetite (Fe3O4) nanoparticles as signal materials. Anti-HBsAg antibodies were labeled with carboxylated Fe3O4 particles with an average diameter of about 150 nm, and an immunochromatography strip was constructed. The detection capability of the assay developed here was 1 ng/mL. In addition, Au-Ag core-shell type metal-enhanced fluorescence (MEF) nanoparticles were introduced as signal materials for the highly sensitive detection of HBsAg in immunochromatographic assay and where a positive result was obtained until 0.01 ng/mL of HBsAg. Furthermore, using the highly specific HIV and HCV recombinant antigens, HBV/HCV/HIV multiplexed immunochromatographic kit was developed.
Magnetic nanoparticles have been used in biochemical and nucleic acid separation and detection, and as magnetic resonance imaging contrast agents in the diagnostic field. Meanwhile, in immunochromatographic assays, gold (Au) nanoparticles are widely used as signal materials. Gold nanoparticles have highly negatively charged surfaces, and therefore, can be conjugated with protein through electrostatic interactions. However, because of their negatively charged surfaces, if the proteins used for labeling are stored in high-salt concentration buffers, gold nanoparticles can be aggregated. Importantly, magnetic nanoparticles have an advantage of being inexpensive compared with gold nanoparticles. Using magnetic nanoparticles, biomolecules can be easily labeled as carboxylated magnetic nanoparticles and, thus, be easily separated by a permanent magnet. In this study, an immunochromatographic assay for the detection of hepatitis B surface antigen (HBsAg) was developed using magnetite (Fe3O4) nanoparticles as signal materials. Anti-HBsAg antibodies were labeled with carboxylated Fe3O4 particles with an average diameter of about 150 nm, and an immunochromatography strip was constructed. The detection capability of the assay developed here was 1 ng/mL. In addition, Au-Ag core-shell type metal-enhanced fluorescence (MEF) nanoparticles were introduced as signal materials for the highly sensitive detection of HBsAg in immunochromatographic assay and where a positive result was obtained until 0.01 ng/mL of HBsAg. Furthermore, using the highly specific HIV and HCV recombinant antigens, HBV/HCV/HIV multiplexed immunochromatographic kit was developed.
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