Purpose: To investigate the antioxidant and anti-inflammatory effects of topical medicinal plant extracts in experimental dry eye (EDE).
Methods: Eye drops consisting of 0.001%, 0.01%, and 0.1% extracts, or balanced salt solution (BSS) were applied in desiccating stress-induced murine dry eye. ...
Purpose: To investigate the antioxidant and anti-inflammatory effects of topical medicinal plant extracts in experimental dry eye (EDE).
Methods: Eye drops consisting of 0.001%, 0.01%, and 0.1% extracts, or balanced salt solution (BSS) were applied in desiccating stress-induced murine dry eye. Tear volume, tear film break-up time (BUT), and corneal fluorescein staining scores were measured 10 days after treatment. Levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, interferon (IFN)-γ, and IFN-γ associated chemokines were measured in the conjunctiva at 10 days using a multiplex immunobead assay. Furthermore, the number of CD4+C-X-C chemokine receptor type 3 (CXCR3)+ T cells using flow cytometry, goblet cell density after periodic acid-Schiff staining, 4-hydroxy-2-nonenal (4-HNE) positive cells staining using immunohistochemistry, and extracellular reactive oxygen species (ROS) production using the 2’,7’-dichlorodihydrofluorescein diacetate assay were performed.
Results: Mice treated with topical application of the 0.1% extract showed significant improvements in all clinical parameters and decreased IL-1β, IL-6, TNF-α, and IFN-γ levels (P < 0.05) compared to the EDE and BSS control groups. In addition, compared to the EDE and BSS control groups, 0.1% extract treated mice showed significant improvement in the number of CD4+CXCR3+ T cells, conjunctival goblet cell density, number of conjunctival 4-HNE-positive cells, and extracellular ROS production (P < 0.05).
Conclusions: Topical administration of 0.1% antioxidant medicinal plant extract improved the clinical parameters, suppressed the expression of inflammatory markers, attenuated oxidative damage, and reduced ROS generation in the ocular surfaces of EDE mice by protecting against oxidative stress and inflammation. These results suggest that eye drops containing antioxidant medicinal plant extracts can be an additional treatment option for dry eye disease.
Purpose: To investigate the antioxidant and anti-inflammatory effects of topical medicinal plant extracts in experimental dry eye (EDE).
Methods: Eye drops consisting of 0.001%, 0.01%, and 0.1% extracts, or balanced salt solution (BSS) were applied in desiccating stress-induced murine dry eye. Tear volume, tear film break-up time (BUT), and corneal fluorescein staining scores were measured 10 days after treatment. Levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, interferon (IFN)-γ, and IFN-γ associated chemokines were measured in the conjunctiva at 10 days using a multiplex immunobead assay. Furthermore, the number of CD4+C-X-C chemokine receptor type 3 (CXCR3)+ T cells using flow cytometry, goblet cell density after periodic acid-Schiff staining, 4-hydroxy-2-nonenal (4-HNE) positive cells staining using immunohistochemistry, and extracellular reactive oxygen species (ROS) production using the 2’,7’-dichlorodihydrofluorescein diacetate assay were performed.
Results: Mice treated with topical application of the 0.1% extract showed significant improvements in all clinical parameters and decreased IL-1β, IL-6, TNF-α, and IFN-γ levels (P < 0.05) compared to the EDE and BSS control groups. In addition, compared to the EDE and BSS control groups, 0.1% extract treated mice showed significant improvement in the number of CD4+CXCR3+ T cells, conjunctival goblet cell density, number of conjunctival 4-HNE-positive cells, and extracellular ROS production (P < 0.05).
Conclusions: Topical administration of 0.1% antioxidant medicinal plant extract improved the clinical parameters, suppressed the expression of inflammatory markers, attenuated oxidative damage, and reduced ROS generation in the ocular surfaces of EDE mice by protecting against oxidative stress and inflammation. These results suggest that eye drops containing antioxidant medicinal plant extracts can be an additional treatment option for dry eye disease.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.