The protective effects of Gastrodia eleta Blume extracts on the liver and kidney from hepatic ischemia/reperfusion injury in rats : 간 허혈/재관류를 유발한 랫드에서 천마 추출물의 간과 신장 보호 효과에 관한 연구원문보기
Lim, Cho Rong
(Graduate School, Eulji University
시니어헬스케어학과 시니어헬스케어학
국내석사)
Hepatic ischemia/reperfusion (I/R) is one of the inevitable processes of liver surgery such as transplantation or liver resection. Hepatic I/R injury is multifactorial and involves direct cellular damages, microcirculatory failure and the inflammatory response to tissue damages that culminate in org...
Hepatic ischemia/reperfusion (I/R) is one of the inevitable processes of liver surgery such as transplantation or liver resection. Hepatic I/R injury is multifactorial and involves direct cellular damages, microcirculatory failure and the inflammatory response to tissue damages that culminate in organ dysfunction and failure. Gastrodia elata Blume (GEB) is an useful traditional herbal medicine used for treatment of headaches, migraine, dizziness, epilepsy, rheumatism, neuralgia, paralysis and other disorders. The aim of this study is to examine the protective effect that GEB and 4-hydroxybenzyl alcohol (HBA), which is one of active ingredients of GEB, ameliorate hepatocyte injury and reduce oxidative stress induced by hepatic I/R injury in rats. Rats were randomized into four different groups; underwent laparotomy without I/R group (Sham), I/R injury group (I/R), treatment of GEB with I/R injured group (GEB), treatment of HBA with I/R injury group (HBA). The GEB or HBA pretreatments were performed orally for 14 days prior to surgery of I/R injury. The left and median lobes of the liver of GEB or HBA treated group and I/R group were occluded with a microvascular clamp for 60 min and the clamp was removed to allow reperfusion. There was reduction of aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen and creatinine levels in the GEB or HBA-treated groups compared with the I/R group. GEB or HBA-treated groups showed decrease of necrosis and infiltration in the liver compared to I/R group. In kidney tissue, GEB or HBA diminished changes of brush bolder in the proximal tubule compared to I/R group. In the GEB or HBA group, expressing tumor necrosis factor-alpha and interleukin-6, and the number of terminal deoxynucelotidyl transferase-mediated dUTP-biotin nick-end labelling positive cells decreased compared to I/R group. The level of malondialdehide was significantly decreased in the GEB or HBA groups compared to I/R group, and activities of superoxide dismutase, an important antioxidant, increased in the GEB or HBA groups compared to I/R group. This study showed GEB or HBA ameliorate I/R induced hepatic injury which may be related to the antioxidant effect of GEB or HBA.
Hepatic ischemia/reperfusion (I/R) is one of the inevitable processes of liver surgery such as transplantation or liver resection. Hepatic I/R injury is multifactorial and involves direct cellular damages, microcirculatory failure and the inflammatory response to tissue damages that culminate in organ dysfunction and failure. Gastrodia elata Blume (GEB) is an useful traditional herbal medicine used for treatment of headaches, migraine, dizziness, epilepsy, rheumatism, neuralgia, paralysis and other disorders. The aim of this study is to examine the protective effect that GEB and 4-hydroxybenzyl alcohol (HBA), which is one of active ingredients of GEB, ameliorate hepatocyte injury and reduce oxidative stress induced by hepatic I/R injury in rats. Rats were randomized into four different groups; underwent laparotomy without I/R group (Sham), I/R injury group (I/R), treatment of GEB with I/R injured group (GEB), treatment of HBA with I/R injury group (HBA). The GEB or HBA pretreatments were performed orally for 14 days prior to surgery of I/R injury. The left and median lobes of the liver of GEB or HBA treated group and I/R group were occluded with a microvascular clamp for 60 min and the clamp was removed to allow reperfusion. There was reduction of aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen and creatinine levels in the GEB or HBA-treated groups compared with the I/R group. GEB or HBA-treated groups showed decrease of necrosis and infiltration in the liver compared to I/R group. In kidney tissue, GEB or HBA diminished changes of brush bolder in the proximal tubule compared to I/R group. In the GEB or HBA group, expressing tumor necrosis factor-alpha and interleukin-6, and the number of terminal deoxynucelotidyl transferase-mediated dUTP-biotin nick-end labelling positive cells decreased compared to I/R group. The level of malondialdehide was significantly decreased in the GEB or HBA groups compared to I/R group, and activities of superoxide dismutase, an important antioxidant, increased in the GEB or HBA groups compared to I/R group. This study showed GEB or HBA ameliorate I/R induced hepatic injury which may be related to the antioxidant effect of GEB or HBA.
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