DNA damage is developed by endogenous and exogenous damage and cells have delicate systems to prevent themselves for the damage. Aberrant DNA and deficient DNA repair are closely associated with various diseases, including aging and cancer. Lately, the incidence of cancer has increased precipitously...
DNA damage is developed by endogenous and exogenous damage and cells have delicate systems to prevent themselves for the damage. Aberrant DNA and deficient DNA repair are closely associated with various diseases, including aging and cancer. Lately, the incidence of cancer has increased precipitously. Many researchers are conducting research to search for cancer prevention materials. Abeliophyllum distichum Nakai, in Oleaceae family, is a Korean endermic plant and has a high research value. Bioactivities of A. distichum such as antioxidant, anti-cancer, and anti-inflammatory studies have been proved through many researches, but researches on its components are insufficient. Acteoside, known to be a lot in Oleaceae, was isolated from A. distichum (AAD) and then this study has progressed to identify protective effects on oxidative DNA damage that is referred to as the cause of various diseases. For the isolation, purification and identification of AAD, we operated Isolera™ Spektra system, HPLC-UV, NMR and LC-MS. Antioxidant activity and protective effects on oxidative DNA damage were progressed at in vitro and in vivo. Consequently, AAD scavenged DPPH (1,1-diphenyl-2-picryl hydrazyl) radical and ABTS {2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt} radical, showing significant ferric ion chelating activity compared with L-ascorbic acid as reducing agents. In plasmid DNA levels, AAD maintained the supercoiled form of oxidatively damaged plasmid DNA and significantly suppressed expression of H2AX and p53 protein and mRNA levels in NIH3T3 cells. Therefore, these protective effects are closely related to the high antioxidant capacity of AAD and the oxidative stress inhibited by radical scavenging activity is thought to affect protein expression and mRNA levels.
In conclusion, in this study, acteoside was isolated, purified and identified from A. distichum, and its antioxidant activity at in vitro level as well as its inhibitory effect on non-cellular and cellular levels of DNA damage was demonstrated. From this study on bioactivities of acteoside isolated from A. distichum, This study suggest that it is valuable as natural additives for food and cosmetics as well as various availabilities as antioxidant and cancer preventive materials.
DNA damage is developed by endogenous and exogenous damage and cells have delicate systems to prevent themselves for the damage. Aberrant DNA and deficient DNA repair are closely associated with various diseases, including aging and cancer. Lately, the incidence of cancer has increased precipitously. Many researchers are conducting research to search for cancer prevention materials. Abeliophyllum distichum Nakai, in Oleaceae family, is a Korean endermic plant and has a high research value. Bioactivities of A. distichum such as antioxidant, anti-cancer, and anti-inflammatory studies have been proved through many researches, but researches on its components are insufficient. Acteoside, known to be a lot in Oleaceae, was isolated from A. distichum (AAD) and then this study has progressed to identify protective effects on oxidative DNA damage that is referred to as the cause of various diseases. For the isolation, purification and identification of AAD, we operated Isolera™ Spektra system, HPLC-UV, NMR and LC-MS. Antioxidant activity and protective effects on oxidative DNA damage were progressed at in vitro and in vivo. Consequently, AAD scavenged DPPH (1,1-diphenyl-2-picryl hydrazyl) radical and ABTS {2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt} radical, showing significant ferric ion chelating activity compared with L-ascorbic acid as reducing agents. In plasmid DNA levels, AAD maintained the supercoiled form of oxidatively damaged plasmid DNA and significantly suppressed expression of H2AX and p53 protein and mRNA levels in NIH3T3 cells. Therefore, these protective effects are closely related to the high antioxidant capacity of AAD and the oxidative stress inhibited by radical scavenging activity is thought to affect protein expression and mRNA levels.
In conclusion, in this study, acteoside was isolated, purified and identified from A. distichum, and its antioxidant activity at in vitro level as well as its inhibitory effect on non-cellular and cellular levels of DNA damage was demonstrated. From this study on bioactivities of acteoside isolated from A. distichum, This study suggest that it is valuable as natural additives for food and cosmetics as well as various availabilities as antioxidant and cancer preventive materials.
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#Abeliophyllum distichum Acteoside DNA damage H2AX
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