Objective : The purpose of this study is to prove effect of Zizyphus jujuba var. inermis leaf on metabolic syndrome and blood circulation and to develop as a new health supplement.
Method
1. ZJL_E (20 mg/ml) was dissolved in 80% ethanol, and we run HPLC analysis.
2. Z. jujuba leaf ...
Objective : The purpose of this study is to prove effect of Zizyphus jujuba var. inermis leaf on metabolic syndrome and blood circulation and to develop as a new health supplement.
Method
1. ZJL_E (20 mg/ml) was dissolved in 80% ethanol, and we run HPLC analysis.
2. Z. jujuba leaf extract (ZJL_E 100 mg/kg) was fed to C57bl/6J mice (♂) for 7 weeks with high-fat diet. Weight and diet intake were measured every week at a regular time. After that, Mice were dissected for blood chemical test. The weight of organs and adipose tissue were measured, and mRNA expression was assayed through RT-PCR after DNA sample manufacture.
3. In vitro : ZJL_E samples (100, 200, 400 μg/ml) with components of Z. jujuba leaf (rutin, isoquercitrin, astragaline (100 μg/ml)) were delivered to washed platelet of SD rats, and incubated in 2 mins. and then we checked platelet aggregation inhibition after platelet agglutination with collagen (10 μg/ml).
In vivo : ZJL_E samples (100, 200 mg/kg) were fed to ICR mice by oral administration. After 7 days, 1% carrageenan with saline solution was injected to the right hind leg of mice. after 72 hours from injection, The length of thrombopoiesis was measured from their tail. After that, we checked platelet aggregation inhibition after platelet agglutination with collagen (10 μg/ml).
Result
1. As a result of HPLC analysis, There were some active components in ZJL_E, which were rutin (18.78 mg/g), hyperoside (20.73 mg/g), isoquercitrin (2.23 mg/g), astragalin (1.17 mg/g).
2. In experimental group treated with ZJL_E 100 mg/kg, there were significant result in weight-loss, FER and LDL-cholesterol, glucos, adiponectin level in blood were decreased. In mRNA expression through RT-PCR, mRNA expression of leptin·UCP-2 in epididymal adipose tissue and adiponectin in liver were significantly decreased. Also as a result of histopathological analysis, the size-increasing of adipocyte in liver and epididymal adipose tissue was inhibited.
3. As a result of in vitro experiment of platelet aggregation inhibition, ZJL_E 100, 200, 400 μg/mL groups have significant platelet aggregation inhibition. Hyperoside, one of active components in ZJL_E, has also dose-dependant platelet aggregation inhibition in 50, 100 μg/mL significantly. As a result of in vivo experiment, the length of tail thrombopoiesis in ZJL_E 200 mg/kg group was significantly shorter than that in control group. In in vitro experiment which proceed in ex-vivo of platelet aggregation inhibition, ZJL_E 100, 200 μg/mL groups have dose-dependant platelet aggregation inhibition compared with control group.
Conclusion : Z. Jujuba Leaf treatment improves body-weight gain, adipose tissue accumulation and dysregulated serum lipid profiles in HFD-induced obese mice by improving metabolism syndrome.
Our result show that Z. Jujuba Leaf is a potent inhibitor of platelet aggregation induced by collagen. In addition, Z. Jujuba Leaf significantly inhibited in vivo thrombus formation.
Objective : The purpose of this study is to prove effect of Zizyphus jujuba var. inermis leaf on metabolic syndrome and blood circulation and to develop as a new health supplement.
Method
1. ZJL_E (20 mg/ml) was dissolved in 80% ethanol, and we run HPLC analysis.
2. Z. jujuba leaf extract (ZJL_E 100 mg/kg) was fed to C57bl/6J mice (♂) for 7 weeks with high-fat diet. Weight and diet intake were measured every week at a regular time. After that, Mice were dissected for blood chemical test. The weight of organs and adipose tissue were measured, and mRNA expression was assayed through RT-PCR after DNA sample manufacture.
3. In vitro : ZJL_E samples (100, 200, 400 μg/ml) with components of Z. jujuba leaf (rutin, isoquercitrin, astragaline (100 μg/ml)) were delivered to washed platelet of SD rats, and incubated in 2 mins. and then we checked platelet aggregation inhibition after platelet agglutination with collagen (10 μg/ml).
In vivo : ZJL_E samples (100, 200 mg/kg) were fed to ICR mice by oral administration. After 7 days, 1% carrageenan with saline solution was injected to the right hind leg of mice. after 72 hours from injection, The length of thrombopoiesis was measured from their tail. After that, we checked platelet aggregation inhibition after platelet agglutination with collagen (10 μg/ml).
Result
1. As a result of HPLC analysis, There were some active components in ZJL_E, which were rutin (18.78 mg/g), hyperoside (20.73 mg/g), isoquercitrin (2.23 mg/g), astragalin (1.17 mg/g).
2. In experimental group treated with ZJL_E 100 mg/kg, there were significant result in weight-loss, FER and LDL-cholesterol, glucos, adiponectin level in blood were decreased. In mRNA expression through RT-PCR, mRNA expression of leptin·UCP-2 in epididymal adipose tissue and adiponectin in liver were significantly decreased. Also as a result of histopathological analysis, the size-increasing of adipocyte in liver and epididymal adipose tissue was inhibited.
3. As a result of in vitro experiment of platelet aggregation inhibition, ZJL_E 100, 200, 400 μg/mL groups have significant platelet aggregation inhibition. Hyperoside, one of active components in ZJL_E, has also dose-dependant platelet aggregation inhibition in 50, 100 μg/mL significantly. As a result of in vivo experiment, the length of tail thrombopoiesis in ZJL_E 200 mg/kg group was significantly shorter than that in control group. In in vitro experiment which proceed in ex-vivo of platelet aggregation inhibition, ZJL_E 100, 200 μg/mL groups have dose-dependant platelet aggregation inhibition compared with control group.
Conclusion : Z. Jujuba Leaf treatment improves body-weight gain, adipose tissue accumulation and dysregulated serum lipid profiles in HFD-induced obese mice by improving metabolism syndrome.
Our result show that Z. Jujuba Leaf is a potent inhibitor of platelet aggregation induced by collagen. In addition, Z. Jujuba Leaf significantly inhibited in vivo thrombus formation.
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