The overall objective of this thesis was to determine the probiotic effects of Lactobacillus fermentum on the brain-related behavior and longevity including genome characteristics by regulating immune response.
Principal characteristics for probiotic candidates are sure to include tolerance to a...
The overall objective of this thesis was to determine the probiotic effects of Lactobacillus fermentum on the brain-related behavior and longevity including genome characteristics by regulating immune response.
Principal characteristics for probiotic candidates are sure to include tolerance to acid and bile, adherence to the intestinal epithelium of the host to survive in the gastrointestinal tract. L. fermentum JDFM216 showed remarkable probiotic abilities that was observed high values of resistance to gastric juice and bile salt, adhesion to mucin surfaces, and bacteriocin production as well as strong antimicrobial activity against tested foodborne pathogens. Characteristics of L. fermentum JDFM216 at the genetic level were analyzed using whole-genome sequencing. Findings of this study on the genetic background of L. fermentum JDFM216 and its potential candidate genes for host longevity and immune response provide new insight for the application of this strain in the food industry as newly isolated functional probiotic.
In this study, functionality of L. fermentum JDFM216 was examined using a C. elegans model to identify the dietary impacts of health-promoting probiotic bacteria. Bacterial colonization in the intestinal tract of C. elegans was determined by plate counting and transmission electron microscopy as well as examined the survival of C. elegans using a solid killing assay. Conditioning with L. fermentum JDFM216 led to its colonization in the nematode intestine and enhanced resistance in nematodes exposed to food-borne pathogens. More importantly, this probiotic strain significantly prolonged the life span of C. elegans. Whole-transcriptome analysis and transgenic worm assays revealed that the health-promoting effects of L. fermentum JDFM216 were mediated by a nuclear hormone receptor (NHR) family and PMK-1 signaling. Therefore, these results describe that a new C. elegans-based system to development novel probiotics and demonstrate that preconditioning with the L. fermentum JDFM216 may positively stimulate the longevity of the C. elegans by enhancing its resistance to foodborne pathogens.
A microbiota is a community of commensal, symbiotic and pathogenic microorganisms, that is includes bacteria, fungi and viruses. These microbiota have been found to be important for immunologic, hormonal and metabolic homeostasis of their host. In this study, behavior tasks and changes of immune system and gut microbiota in old male C57BL/6 mice were evaluated after feeding L. fermentum JDFM216. Importantly, the treatment group of mouse consumed L. fermentum JDFM216 showed an improved patterns of behavior containing memory abilities, capacity for locomotion, sense of balance, and depression. In addition, L. fermentum JDFM216 enhanced phagocytic activity of macrophages and sIgA production. This study suggests that L. fermentum JDFM216 can modulate physiological activity and cognitive function in mice and has also beneficial effects on regulation of immune responses. Moreover, the differences was observed in gut microbiota distribution between groups. L. fermentum JDFM216 may lead to improvement of health with enhanced cognitive, physiology, and immunity by changing gut microbiota.
Until present, while the numerous research has been done to find new functional probiotics, a few was evaluated on the aspects of mechanism, behavior, and longevity. L. fermentum JDFM216 in the current study is not harmful to human and recognized as “Generally Recognized As Safe (GRAS)”. Therefore, L. fermentum JDFM216 could be new functional probiotics as promising food adjuncts for improvement of health associated aging.
The overall objective of this thesis was to determine the probiotic effects of Lactobacillus fermentum on the brain-related behavior and longevity including genome characteristics by regulating immune response.
Principal characteristics for probiotic candidates are sure to include tolerance to acid and bile, adherence to the intestinal epithelium of the host to survive in the gastrointestinal tract. L. fermentum JDFM216 showed remarkable probiotic abilities that was observed high values of resistance to gastric juice and bile salt, adhesion to mucin surfaces, and bacteriocin production as well as strong antimicrobial activity against tested foodborne pathogens. Characteristics of L. fermentum JDFM216 at the genetic level were analyzed using whole-genome sequencing. Findings of this study on the genetic background of L. fermentum JDFM216 and its potential candidate genes for host longevity and immune response provide new insight for the application of this strain in the food industry as newly isolated functional probiotic.
In this study, functionality of L. fermentum JDFM216 was examined using a C. elegans model to identify the dietary impacts of health-promoting probiotic bacteria. Bacterial colonization in the intestinal tract of C. elegans was determined by plate counting and transmission electron microscopy as well as examined the survival of C. elegans using a solid killing assay. Conditioning with L. fermentum JDFM216 led to its colonization in the nematode intestine and enhanced resistance in nematodes exposed to food-borne pathogens. More importantly, this probiotic strain significantly prolonged the life span of C. elegans. Whole-transcriptome analysis and transgenic worm assays revealed that the health-promoting effects of L. fermentum JDFM216 were mediated by a nuclear hormone receptor (NHR) family and PMK-1 signaling. Therefore, these results describe that a new C. elegans-based system to development novel probiotics and demonstrate that preconditioning with the L. fermentum JDFM216 may positively stimulate the longevity of the C. elegans by enhancing its resistance to foodborne pathogens.
A microbiota is a community of commensal, symbiotic and pathogenic microorganisms, that is includes bacteria, fungi and viruses. These microbiota have been found to be important for immunologic, hormonal and metabolic homeostasis of their host. In this study, behavior tasks and changes of immune system and gut microbiota in old male C57BL/6 mice were evaluated after feeding L. fermentum JDFM216. Importantly, the treatment group of mouse consumed L. fermentum JDFM216 showed an improved patterns of behavior containing memory abilities, capacity for locomotion, sense of balance, and depression. In addition, L. fermentum JDFM216 enhanced phagocytic activity of macrophages and sIgA production. This study suggests that L. fermentum JDFM216 can modulate physiological activity and cognitive function in mice and has also beneficial effects on regulation of immune responses. Moreover, the differences was observed in gut microbiota distribution between groups. L. fermentum JDFM216 may lead to improvement of health with enhanced cognitive, physiology, and immunity by changing gut microbiota.
Until present, while the numerous research has been done to find new functional probiotics, a few was evaluated on the aspects of mechanism, behavior, and longevity. L. fermentum JDFM216 in the current study is not harmful to human and recognized as “Generally Recognized As Safe (GRAS)”. Therefore, L. fermentum JDFM216 could be new functional probiotics as promising food adjuncts for improvement of health associated aging.
주제어
#Probiotics Cognitive behavior Immune response Gut microbiota Lactobacillus fermentum genomic analysis longevity Caenorhabditis elegans nuclear hormone receptor family 프로바이오틱스 인지 행동 수명 면역 반응 장내 미생물
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