우량인삼포장에서 2개 균를 분리하여 인삼병원균 6종(Botrytis cinerea, Rhizoctonia solani 9801, Sclerotinia nivalis, Cylindrocarpon destructans, Colectotritum gloeosporioides, ...
우량인삼포장에서 2개 균를 분리하여 인삼병원균 6종(Botrytis cinerea, Rhizoctonia solani 9801, Sclerotinia nivalis, Cylindrocarpon destructans, Colectotritum gloeosporioides, Fusarium solani )과 대치배양한 결과 6.0 ~ 18.0 mm의 균사 생장을 억제하였으며, 형태학적, 생화학적, 16Sr RNA 분석한 결과 B-0528는 B. subtilis, B-1228는 B. amyloliquefaciens 로 동정되어 균주 등록 하였다. 균의 기능성은 siderophore 5~10 mm, AA 는 2.036~7.564㎕/㎖, 인산 가용화 능력이 확인되었으며, 선발균의 생육촉진은 묘삼 지상부의 직경, 경장, 엽폭에서, 특히 혼합처리구에서는 모든 생육이 양호하였다. 결주율은 무처리구(41.3%), LB액(37.3. %)에 비해 B-0528+B-1228 에서는 4%로 낮았다. 묘삼 지하부는 생중, 동체, 동직경이 혼합처리구가 양호했으며, 결주율은 선발균처리구 (0%), LB액(4%), 무처리구(20.3%)였다. 농도별 발아율은 B-0528은 1 ppm에서 70%, B-1228은 10ppm 에서 83.3% 였다. R. solani 9801 처리후 선발균의 병억제 효과는 B-1228(15%)이 결주율이 낮았다. 따라서 B-1228는 인삼뿌리썩음병에 대한 생물학적 방제제로서 가능성이 높을 것으로 판단된다.
우량인삼포장에서 2개 균를 분리하여 인삼병원균 6종(Botrytis cinerea, Rhizoctonia solani 9801, Sclerotinia nivalis, Cylindrocarpon destructans, Colectotritum gloeosporioides, Fusarium solani )과 대치배양한 결과 6.0 ~ 18.0 mm의 균사 생장을 억제하였으며, 형태학적, 생화학적, 16Sr RNA 분석한 결과 B-0528는 B. subtilis, B-1228는 B. amyloliquefaciens 로 동정되어 균주 등록 하였다. 균의 기능성은 siderophore 5~10 mm, AA 는 2.036~7.564㎕/㎖, 인산 가용화 능력이 확인되었으며, 선발균의 생육촉진은 묘삼 지상부의 직경, 경장, 엽폭에서, 특히 혼합처리구에서는 모든 생육이 양호하였다. 결주율은 무처리구(41.3%), LB액(37.3. %)에 비해 B-0528+B-1228 에서는 4%로 낮았다. 묘삼 지하부는 생중, 동체, 동직경이 혼합처리구가 양호했으며, 결주율은 선발균처리구 (0%), LB액(4%), 무처리구(20.3%)였다. 농도별 발아율은 B-0528은 1 ppm에서 70%, B-1228은 10ppm 에서 83.3% 였다. R. solani 9801 처리후 선발균의 병억제 효과는 B-1228(15%)이 결주율이 낮았다. 따라서 B-1228는 인삼뿌리썩음병에 대한 생물학적 방제제로서 가능성이 높을 것으로 판단된다.
Ginseng (Panax ginseng) is one of the most widely cultivated medicinal herbs in Korea. However, the yield loss reaches up to 30-60% due to the various diseases. Therefore, the successful disease control is critical for increasing ginseng production. This study aims to isolate potential biocontrol ag...
Ginseng (Panax ginseng) is one of the most widely cultivated medicinal herbs in Korea. However, the yield loss reaches up to 30-60% due to the various diseases. Therefore, the successful disease control is critical for increasing ginseng production. This study aims to isolate potential biocontrol agents from the field with high ginseng yield. Among the different microbes isolated, two different bacterial strains (B-0528 and B-1228) markedly inhibited mycelial growth of the major ginseng pathogens, such as Cylindrocarpon destructans, Botrytis cinera, Rhizoctonia solani 9801, Sclerotinia nivalis, Colletotrichum gloeosporioides and Fusarium solani KACC44981. Morphological and biochemical analyses along 16S rRNA sequence showed that B-0528 and B-1228 strains were identified as Bacillus subtilis and Bacillus amyloliquefaciens, respectively. Both B. subtilis and B. amyloliquefaciens produced siderophore with the length of 5 ~ 10 mm or more, and accumulated IAA (indole-3-acetic acid ) up to 2.036~7.564㎕/㎖. Treatment of 1-year-old ginseng seedlings with B-0528 (108cfu/ml) and B-1228 (108cfu/ml) significantly increased the length of stems and leaves, thus enhancing the height of the ginseng seedlings. Missing ratio was also strongly induced from 41.3% (untreated) or 37.3% (medium-treated) to 4% (B-0528+B-1228-treated). Co-treatment of B-0528 and B-122\8 induced significant differences in the percentage of fresh weight, root length, and root diameters compared to the untreated group. Missing ratio was also reduced from the seedlings treated with B-0528+B-1228 (0%) compared to the untreated control (20.3%) or medium-treated ones (4.0%). Interestingly, B-1228 treatment exhibited the highest control efficacy against damping-off caused by Rhizoctonia solani 9801 compared to B-0528 or co-treatment (B-0528+ B-1228). Finally the germination rate of B-1228-treated ginseng seed was 71.7% and 83.3% at 1 ppm and 10 ppm, respectively, while untreated one showed 53.3% germination rate after 100 days. Taken together, these results indicate that B-1228 can be used as an effective biocontrol agent for cultivating ginseng crop.
Ginseng (Panax ginseng) is one of the most widely cultivated medicinal herbs in Korea. However, the yield loss reaches up to 30-60% due to the various diseases. Therefore, the successful disease control is critical for increasing ginseng production. This study aims to isolate potential biocontrol agents from the field with high ginseng yield. Among the different microbes isolated, two different bacterial strains (B-0528 and B-1228) markedly inhibited mycelial growth of the major ginseng pathogens, such as Cylindrocarpon destructans, Botrytis cinera, Rhizoctonia solani 9801, Sclerotinia nivalis, Colletotrichum gloeosporioides and Fusarium solani KACC44981. Morphological and biochemical analyses along 16S rRNA sequence showed that B-0528 and B-1228 strains were identified as Bacillus subtilis and Bacillus amyloliquefaciens, respectively. Both B. subtilis and B. amyloliquefaciens produced siderophore with the length of 5 ~ 10 mm or more, and accumulated IAA (indole-3-acetic acid ) up to 2.036~7.564㎕/㎖. Treatment of 1-year-old ginseng seedlings with B-0528 (108cfu/ml) and B-1228 (108cfu/ml) significantly increased the length of stems and leaves, thus enhancing the height of the ginseng seedlings. Missing ratio was also strongly induced from 41.3% (untreated) or 37.3% (medium-treated) to 4% (B-0528+B-1228-treated). Co-treatment of B-0528 and B-122\8 induced significant differences in the percentage of fresh weight, root length, and root diameters compared to the untreated group. Missing ratio was also reduced from the seedlings treated with B-0528+B-1228 (0%) compared to the untreated control (20.3%) or medium-treated ones (4.0%). Interestingly, B-1228 treatment exhibited the highest control efficacy against damping-off caused by Rhizoctonia solani 9801 compared to B-0528 or co-treatment (B-0528+ B-1228). Finally the germination rate of B-1228-treated ginseng seed was 71.7% and 83.3% at 1 ppm and 10 ppm, respectively, while untreated one showed 53.3% germination rate after 100 days. Taken together, these results indicate that B-1228 can be used as an effective biocontrol agent for cultivating ginseng crop.
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