Abstract ▼ AI-Helper

This study was conducted to investigate the content of lutein in perilla leaves(Perilla frutescens var. japonica Hara) in January, April, July and October. The antioxidative activities of Acetone-MeOH (1: 1), 70% EtOH extract and lutein were measured. The antioxidative activity of DPPH radical scave...

This study was conducted to investigate the content of lutein in perilla leaves(Perilla frutescens var. japonica Hara) in January, April, July and October. The antioxidative activities of Acetone-MeOH (1: 1), 70% EtOH extract and lutein were measured. The antioxidative activity of DPPH radical scavenging ability, ABTS radical scavenging ability, hydroxy radical scavenging ability, Fe2+ chelating scavenging ability and nitrite scavenging ability were investigated at the concentration of 100 ug/mL. The results are as follow.

1. Lutein contents were higher in July, followed by perilla leaf (604 mg/kg)>January perilla leaf (220.26 mg/kg)>April perilla leaf (181. 35mg/kg)>October perilla leaf (105.12 mg/kg). 70% EtOH extracts were higher in July than in July perilla leaf (1984.81 mg/kg)>January perilla leaf (811.79 mg/kg)>April perilla leaf (337.41 mg/kg)> October perilla leaf (331.81 mg/kg). Lutein contents were different according to seasons, but the lutein contents of the perilla leaf was the highest in July.

2. The electron donating ability was 56.37±0.08% in the lutein standard material and 40.15±3.69% in the 1:1 extract of Acetone-MeOH in July, and 78.25±0.99% in the 70% EtOH extract. Ascorbic acid was 88.13 ± 0.17%.

3. The ABTS+ radical scavenging activity was 48.66±0.88% in lutein and 52.17±0.50% in the 1:1 extract of Acetone-MeOH, and 94.52 ±0.28% in the EtOH extract of 70% EtOH. Ascorbic acid was 95.92±0.11%.

4. The hydroxyl radical scavenging activity was 37.01±1.16% for lutein and 15.02±0.55% in the 1:1 extract of Acetone-MeOH and 37.05±0.34% in the EtOH extract of 70% EtOH. Ascorbic acid as a control was 80.73 ± 0.34%.

5. Fe²+ chelating ability was 84.61±0.45% in lutein and 11.48±0.39% in Acetone-MeOH extract and 20.29±0.48% in 70% EtOH extract.
Deferoxamine as a control was 99.43±0.39%.


6. Nitrite scavenging activity increased with decreasing pH. The nitrite scavenging activity of lutein was 97.63±0.15%, 93.23±1.65% and 83.72±1.03% at pH 1.2, pH 2.5 and pH 4.2, respectively. The results were higher than 82.44±0.25%, 65.98±1.03% and 61.80±0.89% of BHT, respectively.
Nitrite scavenging activity at pH 1.2 and pH 2.5 was 92.28±0.15% and 88.77±0.54%, respectively, in the extract of Acetone-MeOH extract from July, and 94.74±0.39% and 92.37±0.15% respectively in the 70% EtOH extract. The results were higher than those of BHT with nitrite scavenging ability of 82.44±0.25% and 65.98±1.03%.