This study was conducted to investigate the antioxidant activity and antiinflammatory activity of 70% ethanol extracts after seasonally harvested perilla leaf cultivated house in Chubu area, Geumsan. The results were as follow.
The yield of 70% ethanol extract of perilla leaves was 11.2% in...
This study was conducted to investigate the antioxidant activity and antiinflammatory activity of 70% ethanol extracts after seasonally harvested perilla leaf cultivated house in Chubu area, Geumsan. The results were as follow.
The yield of 70% ethanol extract of perilla leaves was 11.2% in January (winter), 14.3% in April (spring), 14.9% in July (summer) and 14.0% in October (autumn).
Rosmarinic acid content of perilla leaves was 83.45 mg/g in January, 44.74 mg/g in April, 117.40 mg/g in July and 26.41 mg/g in October. The content of rosmarinic acid in the perilla leaves was the highest at 117.40 mg/g in July (summer).
Total polyphenol contents of 70% ethanol extract of perilla leaves were 231.20 mg/100g in January, 119.41 mg/100g in April, 280.51 mg/100g in July and 96.28 mg/100g in October. The contents were in the order of July > January > April > October, and it was highest in the perilla leaf collected in July (summer).
The total flavonoid content of 70% ethanol extract of perilla leaf was 638.13 mg/100g in January, 243.04 mg/100g in April, 806.87 mg/100g in July and 180.13 mg/100g in October. The contents were in the order of July > January > April > October. The flavonoid content of perilla leaves was the highest at 806.87 mg/100g in July (summer).
The electron donating ability(DPPH) of 70% ethanol extract of perilla leaf (100 μg/mL concentration) was 87.61% in January, 46.94% in April, 91.97% in July and 35.37% in October. Perilla leaves collected in July (summer) were highest at 91.97% and higher than 90.56% of ascorbic acid.
At 100 μg/mL concentration, the ABTS radical scavenging ability of the 70% ethanol extract of perilla leaf was in order of July (95.84%) > January (95.63%)> April (77.84%)> October (63.41%). Perilla leaf extracts collected in the summer (January) and winter (January) were 95.84% and 95.63%, similar to 95.41% of ascorbic acid.
At the concentration of 100 μg/mL, the hydroxyl radical scavenging activity of 70% ethanol extract of perilla leaves was 24.49% ~ 31.55% and lower than 80.58% of ascorbic acid.
At the concentration of 100 μg/mL, the Fe2+ chelating ability of 70% ethanol extract of perilla leaves was in April(62.77%), July (62.63%), January(60.58%) and October(57.98%). It was lower than 95.93% of DFX.
The nitrite scavenging activity of perilla leaves was increased in the order of pH 1.2> pH 2.5> pH 6.0. In the case of pH 1.2 and 2.5, nitrite scavenging ability of perilla leaf extract was 81.67 ~ 96.36%, which was higher than 74.77% and 74.01% of BHT.
When LPS extracts were treated at 100, 200 and 400 μg/mL, the NO production by LPS was decreased to 15.43 μM, 12.78 μM and 11.03 μM, respectively. perilla leaf extract inhibited NO production in macrophages and showed anti-inflammatory effects.
Perilla leaf extract (July) inhibited the expression of iNOS protein and COX-2 protein in a concentration-dependent manner.
Perilla leaf extracts (July) inhibited the signaling pathways of NF-κB and MAPKs (ERK1/2, JNK, p38) and regulated the expression of inflammatory mediators.
In the mouse serum with LPS-induced inflammation, the perilla leaf extract significantly decreased the production of TNF-α, IL-1β and IL-6, which are inflammatory cytokines, and IL-10 was increased compared to the control group.
In conclusion, rosmarinic acid content was different and antioxidant activity was different according to the harvest season. In addition, rosmarinic acid content and antioxidant activity were highest in the sesame leaf extracts of July (summer), so rosmarinic acid content and antioxidant activity were correlated.
Antioxidant activity was highest in July, and it was confirmed that NO and iNOS and COX-2 protein expression were inhibited by anti-inflammatory test with extracts of sesame leaf extract.
In addition, it will have effect on the relief of inflammatory response by controlling NO as inflammatory mediator and inflammatory-cytokines etc.
These results suggest that perilla leaf extract has antioxidant, anti-inflammatory and various physiological activities and it is necessary to study chemical composition and efficacy for bioactive material.
This study was conducted to investigate the antioxidant activity and antiinflammatory activity of 70% ethanol extracts after seasonally harvested perilla leaf cultivated house in Chubu area, Geumsan. The results were as follow.
The yield of 70% ethanol extract of perilla leaves was 11.2% in January (winter), 14.3% in April (spring), 14.9% in July (summer) and 14.0% in October (autumn).
Rosmarinic acid content of perilla leaves was 83.45 mg/g in January, 44.74 mg/g in April, 117.40 mg/g in July and 26.41 mg/g in October. The content of rosmarinic acid in the perilla leaves was the highest at 117.40 mg/g in July (summer).
Total polyphenol contents of 70% ethanol extract of perilla leaves were 231.20 mg/100g in January, 119.41 mg/100g in April, 280.51 mg/100g in July and 96.28 mg/100g in October. The contents were in the order of July > January > April > October, and it was highest in the perilla leaf collected in July (summer).
The total flavonoid content of 70% ethanol extract of perilla leaf was 638.13 mg/100g in January, 243.04 mg/100g in April, 806.87 mg/100g in July and 180.13 mg/100g in October. The contents were in the order of July > January > April > October. The flavonoid content of perilla leaves was the highest at 806.87 mg/100g in July (summer).
The electron donating ability(DPPH) of 70% ethanol extract of perilla leaf (100 μg/mL concentration) was 87.61% in January, 46.94% in April, 91.97% in July and 35.37% in October. Perilla leaves collected in July (summer) were highest at 91.97% and higher than 90.56% of ascorbic acid.
At 100 μg/mL concentration, the ABTS radical scavenging ability of the 70% ethanol extract of perilla leaf was in order of July (95.84%) > January (95.63%)> April (77.84%)> October (63.41%). Perilla leaf extracts collected in the summer (January) and winter (January) were 95.84% and 95.63%, similar to 95.41% of ascorbic acid.
At the concentration of 100 μg/mL, the hydroxyl radical scavenging activity of 70% ethanol extract of perilla leaves was 24.49% ~ 31.55% and lower than 80.58% of ascorbic acid.
At the concentration of 100 μg/mL, the Fe2+ chelating ability of 70% ethanol extract of perilla leaves was in April(62.77%), July (62.63%), January(60.58%) and October(57.98%). It was lower than 95.93% of DFX.
The nitrite scavenging activity of perilla leaves was increased in the order of pH 1.2> pH 2.5> pH 6.0. In the case of pH 1.2 and 2.5, nitrite scavenging ability of perilla leaf extract was 81.67 ~ 96.36%, which was higher than 74.77% and 74.01% of BHT.
When LPS extracts were treated at 100, 200 and 400 μg/mL, the NO production by LPS was decreased to 15.43 μM, 12.78 μM and 11.03 μM, respectively. perilla leaf extract inhibited NO production in macrophages and showed anti-inflammatory effects.
Perilla leaf extract (July) inhibited the expression of iNOS protein and COX-2 protein in a concentration-dependent manner.
Perilla leaf extracts (July) inhibited the signaling pathways of NF-κB and MAPKs (ERK1/2, JNK, p38) and regulated the expression of inflammatory mediators.
In the mouse serum with LPS-induced inflammation, the perilla leaf extract significantly decreased the production of TNF-α, IL-1β and IL-6, which are inflammatory cytokines, and IL-10 was increased compared to the control group.
In conclusion, rosmarinic acid content was different and antioxidant activity was different according to the harvest season. In addition, rosmarinic acid content and antioxidant activity were highest in the sesame leaf extracts of July (summer), so rosmarinic acid content and antioxidant activity were correlated.
Antioxidant activity was highest in July, and it was confirmed that NO and iNOS and COX-2 protein expression were inhibited by anti-inflammatory test with extracts of sesame leaf extract.
In addition, it will have effect on the relief of inflammatory response by controlling NO as inflammatory mediator and inflammatory-cytokines etc.
These results suggest that perilla leaf extract has antioxidant, anti-inflammatory and various physiological activities and it is necessary to study chemical composition and efficacy for bioactive material.
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