Biotin that makes up nails and skin is a type of vitamin and is called vitamin B7 or vitamin H. Biotin is rich in animal liver, egg yolk and nuts and also in cosmetic products that help healthy skin and hair. Compared to the efficacy and function of biotin in cosmetics and ingredients, there is few ...
Biotin that makes up nails and skin is a type of vitamin and is called vitamin B7 or vitamin H. Biotin is rich in animal liver, egg yolk and nuts and also in cosmetic products that help healthy skin and hair. Compared to the efficacy and function of biotin in cosmetics and ingredients, there is few research on biotin analysis in Korea. Although biotin content analysis is recommended by the Ministry of Food and Drug Safety, it is difficult to be used as basic data for research on cosmetics. There are several problems with biotin analysis with licensed assays. First, the large particle size of the beads in column affects the detection peak resolution. Second, it takes a long time due to low flow rate and low temperature. Third, accurate content analysis is difficult due to overlapped peaks. Because of these problems, we changed four conditions (flow rate, temperature, stationary phase, and mobile phase) to optimize for analysis of biotin and improve for the disadvantages of the existing biotin assay. As a result, increasing the flow rate and temperature, shortened the elution time and improved the resolution the elution time shortened and the resolution improved. Also, the peak overlap was improved. The stationary phase was changed from C18 (250 mm × 4.6 mm, 5 μm) to C8 (250 mm × 4.6 mm, 3.5 μm). As a result, the elution time was not significantly different, but the resolution was improved by changing the particle size. As the proportion of non-polar acetonitrile in the mobile phase increased, the elution time increased, but the sample was not separated well. This is because the elution was improved due to the increase of the non-polar solvent caused the overlap of the peak. The ratio of water and acetonitrile containing 0.1% TFA showed the best elution time and resolution at 80 to 20.
In this study, we analyze biotin using HPLC improved method that was able to efficient analysis and accurate database construction. It enabled to reduce analyzing time and increase resolution by changing HPLC conditions of flow, temperature, mobile phase and column type. These changes will enable efficient internal quality management in cosmetics fields. This study can be used as a good reference for those who study and analyze biotin in functional cosmetics.
Biotin that makes up nails and skin is a type of vitamin and is called vitamin B7 or vitamin H. Biotin is rich in animal liver, egg yolk and nuts and also in cosmetic products that help healthy skin and hair. Compared to the efficacy and function of biotin in cosmetics and ingredients, there is few research on biotin analysis in Korea. Although biotin content analysis is recommended by the Ministry of Food and Drug Safety, it is difficult to be used as basic data for research on cosmetics. There are several problems with biotin analysis with licensed assays. First, the large particle size of the beads in column affects the detection peak resolution. Second, it takes a long time due to low flow rate and low temperature. Third, accurate content analysis is difficult due to overlapped peaks. Because of these problems, we changed four conditions (flow rate, temperature, stationary phase, and mobile phase) to optimize for analysis of biotin and improve for the disadvantages of the existing biotin assay. As a result, increasing the flow rate and temperature, shortened the elution time and improved the resolution the elution time shortened and the resolution improved. Also, the peak overlap was improved. The stationary phase was changed from C18 (250 mm × 4.6 mm, 5 μm) to C8 (250 mm × 4.6 mm, 3.5 μm). As a result, the elution time was not significantly different, but the resolution was improved by changing the particle size. As the proportion of non-polar acetonitrile in the mobile phase increased, the elution time increased, but the sample was not separated well. This is because the elution was improved due to the increase of the non-polar solvent caused the overlap of the peak. The ratio of water and acetonitrile containing 0.1% TFA showed the best elution time and resolution at 80 to 20.
In this study, we analyze biotin using HPLC improved method that was able to efficient analysis and accurate database construction. It enabled to reduce analyzing time and increase resolution by changing HPLC conditions of flow, temperature, mobile phase and column type. These changes will enable efficient internal quality management in cosmetics fields. This study can be used as a good reference for those who study and analyze biotin in functional cosmetics.
주제어
#HPLC, Biotin
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