To overcome side effects of acne medications, many researches on natural antiacne compounds are actively being conducted. Here, we tried to isolate and identify antifungal compounds against three strains (Cutibacterium acnes, Staphylococcus aureus, Bacillus subtilis) from Mori cortex. Mori cortex wa...
To overcome side effects of acne medications, many researches on natural antiacne compounds are actively being conducted. Here, we tried to isolate and identify antifungal compounds against three strains (Cutibacterium acnes, Staphylococcus aureus, Bacillus subtilis) from Mori cortex. Mori cortex was extracted with 80% (v/v) ethanol for 10 days. The extracts were purified by solvent fractionation, silica gel column chromatography and HPLC. The purified compound was identified for the active peak with 1H-NMR and GC-MSD. Each fraction was evaluated using a agar well diffusion method and susceptibility test. Positive control was used isotretinoin, triclosan, erythromycin and clindamycin. Among the four solvent fractions, ethyl acetate fraction (MEF) showed the best antifungal activity, and had Inhibition zone of 20.3 mm, 15.2 mm, and 16.2 mm for C. acnes, S. aureus and B. subtilis, respectively. Thus, MHF has been further purified by silica gel column chromatography and Prep-HPLC. Among the twelve silica gel column chromatography fractions, MEF 11 showed the best antifungal activity, and had Inhibition zone of 24.5 mm, 16.3 mm and 16.4 mm for C. acnes, S. aureus and B. subtilis, respectively. Among the HPLC fractions, MEF 11-6 showed the best antifungal activity and had inhibitory zone of 24.5 mm, 16.3 mm and 16.4 mm for C. acnes, S. aureus and B. subtilis, respectively. Isoretinoin, triclosan, erythromycin, and clindamycin, which were used as positive controls, had 15.3 mm, 25.2 mm, 25.8 mm, and 26 mm inhibitory zone for C. acnes, respectively. Analysis of MEF 11-6 with 1H-NMR and GC-MSD revealed that the structure of the isolated compound was resorcinol. MIC50 values of resorcinol were 0.058 μg/mL, 0.96 μg/mL and 1.92 μg/mL for C. acnes, S. aureus and B. subtilis, respectively. MIC50 values of isotretinoin were 0.12 μg/mL, 4 μg/mL, and 1.1 μg/mL for C. acnes, S. aureus and B. subtilis, respectively, and the MIC50 values of erythromycin were 0.059 μg/mL, 0.86 μg/mL, 0.45 μg/mL, respectively. It was confirmed that resorcinol had higher susceptibility than isotretinoin and erythromycin. These data suggest that Mori cortex extracts and its isolated resorcinol are good candidates for acne medications or medical, beauty treatment.
To overcome side effects of acne medications, many researches on natural antiacne compounds are actively being conducted. Here, we tried to isolate and identify antifungal compounds against three strains (Cutibacterium acnes, Staphylococcus aureus, Bacillus subtilis) from Mori cortex. Mori cortex was extracted with 80% (v/v) ethanol for 10 days. The extracts were purified by solvent fractionation, silica gel column chromatography and HPLC. The purified compound was identified for the active peak with 1H-NMR and GC-MSD. Each fraction was evaluated using a agar well diffusion method and susceptibility test. Positive control was used isotretinoin, triclosan, erythromycin and clindamycin. Among the four solvent fractions, ethyl acetate fraction (MEF) showed the best antifungal activity, and had Inhibition zone of 20.3 mm, 15.2 mm, and 16.2 mm for C. acnes, S. aureus and B. subtilis, respectively. Thus, MHF has been further purified by silica gel column chromatography and Prep-HPLC. Among the twelve silica gel column chromatography fractions, MEF 11 showed the best antifungal activity, and had Inhibition zone of 24.5 mm, 16.3 mm and 16.4 mm for C. acnes, S. aureus and B. subtilis, respectively. Among the HPLC fractions, MEF 11-6 showed the best antifungal activity and had inhibitory zone of 24.5 mm, 16.3 mm and 16.4 mm for C. acnes, S. aureus and B. subtilis, respectively. Isoretinoin, triclosan, erythromycin, and clindamycin, which were used as positive controls, had 15.3 mm, 25.2 mm, 25.8 mm, and 26 mm inhibitory zone for C. acnes, respectively. Analysis of MEF 11-6 with 1H-NMR and GC-MSD revealed that the structure of the isolated compound was resorcinol. MIC50 values of resorcinol were 0.058 μg/mL, 0.96 μg/mL and 1.92 μg/mL for C. acnes, S. aureus and B. subtilis, respectively. MIC50 values of isotretinoin were 0.12 μg/mL, 4 μg/mL, and 1.1 μg/mL for C. acnes, S. aureus and B. subtilis, respectively, and the MIC50 values of erythromycin were 0.059 μg/mL, 0.86 μg/mL, 0.45 μg/mL, respectively. It was confirmed that resorcinol had higher susceptibility than isotretinoin and erythromycin. These data suggest that Mori cortex extracts and its isolated resorcinol are good candidates for acne medications or medical, beauty treatment.
주제어
#상백피 Cutibacterium acnes Staphylococcus aureus Bacillus subtilis Antifungal 여드름 항여드름
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