초록

AI-Helper

免疫抗體分子의 基本 構造成分인 H-chain, L-chain외에 重合抗體分子 속에서만 발견되는 이른바, J-chain의 構造와 機能을 밝히기 위한 前段階로 J-chain의 純粹分離를 試圖하였다. 우선 多發性骨髓腫 患者의 血淸으로부터 重合型 IgA를 純粹分離한후, 이를 환원시켜 L-J-chain 混合物을 얻은 다음 3가지 方法, 1) 제조용 디스크 전기영동법 2) 脫鹽 透析法, 3) 이온-교환 크로마토그라피법으로 J-chain을 순수분리할 수 있었다. 위 3가지 방법으로 분리한 J-chain의 物理化學的 및 生化學的 성상은 동일하였으며, 사용한 세가지 방법 중 脫鹽 透析法이 가장 간편하고 효과적인 方法임을 알았다.

Abstract ▼ AI-Helper

J-chain was purified from human serum IgA by three different methods which involve preparative disc electrophoresis, ion exchange chromatography and water precipitation. It was found that the simplest and satisfactory method was precipitation in water. All the J-chain preparations obtained from the...

J-chain was purified from human serum IgA by three different methods which involve preparative disc electrophoresis, ion exchange chromatography and water precipitation. It was found that the simplest and satisfactory method was precipitation in water. All the J-chain preparations obtained from the above different procedures showed similar electrophoretic mobility and band-patterns.