초록

AI-Helper

인삼 crown gall tumor callus의 특성을 구명하기 위하여 Agrobacterium tumefaciens C58을 감염시켜서 얻어진 crown gall tumor를 phytohormone 무첨가 배지에서 배양하여 생육이 왕성한 tumor callus를 유기하였다. 이들 tumor callus는 정상조직에서 유기한 callus에 비해 외관상으로는 friable한 형태의 덩어리로 자라면서도 매우 단단한 양상을 보였으며, callus증식에 적합한 phytohormone 농도에서는 tumor callus의 생육이 오히려 억제되는 현상을 보였다. Tumor callus의 생육은 고체배양시 암배양이 광배양보다 생체중의 경우에는 2.4배, 건물중은 1.9배 가량이 더 많으며, 2,4-D가 함유된 최적조건에서 배양한 정상 callus의 생체중과는 비슷한 경향을 보였으나, 건물중은 tumor callus가 더 높았다. 그리고 현탁배양시에는 tumor callus가 정상 callus보다 생장속도가 2배 이상 빠른 경향이었다.

Abstract ▼ AI-Helper

Grown-gall tumor was induced from the infection of Panax ginseng C.A. Meyer by Agrobacterium tumefaciens C58 and the tumor calli were formed on the phytohormone free MS medium. The calli were friable and rough in appearance. Calli obtained from crown gall tumor were similar to and indistinguishable ...

Grown-gall tumor was induced from the infection of Panax ginseng C.A. Meyer by Agrobacterium tumefaciens C58 and the tumor calli were formed on the phytohormone free MS medium. The calli were friable and rough in appearance. Calli obtained from crown gall tumor were similar to and indistinguishable from each other. The tumor callus was quite different from normal callus. Tumor callus grew rapidly, whereas mal callus appeared late. The growth of tumor callus was better in the dark than in the light. In suspension culture, the fresh weight of tumor callus was twice as much in comparison with normal callus.