In March of 2003, tumors (galls) were observed on ginseng seedling roots in ginseng seedbeds at Yeoju, Gyeonggi province, Korea. Symptoms were spherical or galls with about 0.5-1.0cm in diameter formed on the upper through middle parts of the primary roots. Bacterial isolates obtained from the root ...
In March of 2003, tumors (galls) were observed on ginseng seedling roots in ginseng seedbeds at Yeoju, Gyeonggi province, Korea. Symptoms were spherical or galls with about 0.5-1.0cm in diameter formed on the upper through middle parts of the primary roots. Bacterial isolates obtained from the root galls were Gram-negative, rod-shaped with peritrichous flagella, aerobic, not forming yellow or orange colonies on nutrient glucose agar, yeast extract-dextrose $CaCO_3$ agar and nutrient-broth yeast extract agar, non-fluorescent on King's B agar, and non-spore forming, which were identical to characteristics of the genus Agrobacterium. They were identified as Agrobacterium tumefaciens with 0.732-0.993 similarities in 100% probability by the Biolog analyses. The 16S rRNA gene partial sequences of the six isolates tested (Genbank Accession EF486308-EF486313) were 100% homologous to those of other A. tumefaciens strains (GenBank accession AF501343, AY701900, AY701898, AY701899). The above results confirmed that this bacterium is A. tumefaciens. Pathogenicity of the bacteria was proved by the inoculation test on carrot root discs and tomato seedlings. This is the first description of A. tumefaciens causing root gall in ginseng seedling. The disease occurred locally and sparsely, but considering its appearances in seedbeds suggests that the ginseng root gall may become a threat to ginseng in Korea.
In March of 2003, tumors (galls) were observed on ginseng seedling roots in ginseng seedbeds at Yeoju, Gyeonggi province, Korea. Symptoms were spherical or galls with about 0.5-1.0cm in diameter formed on the upper through middle parts of the primary roots. Bacterial isolates obtained from the root galls were Gram-negative, rod-shaped with peritrichous flagella, aerobic, not forming yellow or orange colonies on nutrient glucose agar, yeast extract-dextrose $CaCO_3$ agar and nutrient-broth yeast extract agar, non-fluorescent on King's B agar, and non-spore forming, which were identical to characteristics of the genus Agrobacterium. They were identified as Agrobacterium tumefaciens with 0.732-0.993 similarities in 100% probability by the Biolog analyses. The 16S rRNA gene partial sequences of the six isolates tested (Genbank Accession EF486308-EF486313) were 100% homologous to those of other A. tumefaciens strains (GenBank accession AF501343, AY701900, AY701898, AY701899). The above results confirmed that this bacterium is A. tumefaciens. Pathogenicity of the bacteria was proved by the inoculation test on carrot root discs and tomato seedlings. This is the first description of A. tumefaciens causing root gall in ginseng seedling. The disease occurred locally and sparsely, but considering its appearances in seedbeds suggests that the ginseng root gall may become a threat to ginseng in Korea.
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데이터처리
Direct sequencing of the amplified PCR products was done using ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Model ABI 3700). The sequences obtained from the isolates were used for sequence database searches in GenBank and sequence similarities to the known A. tumefaciens strains were measured using the BLAST program. Sequences were assembled using the SeqMan program and analyzed with the MegAlign programs (DNASTAR, Inc.
이론/모형
tumefaciens strains were measured using the BLAST program. Sequences were assembled using the SeqMan program and analyzed with the MegAlign programs (DNASTAR, Inc., USA).
성능/효과
There were also variations in the utilization of 21 carbon sources amonthe 6 isolates. The Biolog data gave the Agrobacterium isolates tested high similarities to A. tumefaciens (similarities of 0.732-0.993% with a match probability of 100%). Thus, based on the results of the above cultural and physiological characteristics of the bacterial isolates and their Biolog analyses, the 6 bacterial isolates tested were confirmed to be A.
The partial sequences of 16S rRNA gene of all isolates tested contained the same 409 bases (data not shown), and BLAST analyses showed that they had 100% homology to those of other 4 tumefaciens strains (GenBank accession numbers AF501343, AY701900, AY701898, and AY701899) that had been deposited in the GenBank database. These results may also confirm that the bacterial isolates are all A.
참고문헌 (18)
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