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Abstract ▼ AI-Helper

Changes in leucinaminopeptidase (LAP) activity and rymograms and protein patterns during metamorphosis were investigated in haemolymph of Menelaides alcinous. The results obtained are as follows; 1. LAP activity was highest at mature larva, decreased at prepupa and increased again at early pupal st...

Changes in leucinaminopeptidase (LAP) activity and rymograms and protein patterns during metamorphosis were investigated in haemolymph of Menelaides alcinous. The results obtained are as follows; 1. LAP activity was highest at mature larva, decreased at prepupa and increased again at early pupal stage. This characteristic changing aspect is considered to be responsible for the food intake and histolysis of larval tissues. 2. The LAP zymograms also changed remarkably in relation to ontogenesis during the larval-pupal transformation. 3. A total of 36 protein bands and 154-186 protein spots have been detected between molecular weight 669-14 kd throughout the development. 4. Among the above protein bands and spots, 19 bands and 102 spots appeared constantly in all the stages. In particular, some of the other spot groups which seem to reflect the histolysis of larval tissues and histogenesis of adult tissues were appeared or disappeared in specific stages.