초록

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Abstract ▼ AI-Helper

Just upstream of the gene 10 in bacteriophage T7 genome there exist expression regulatory sequences for phage T7 RNA polymerase transcription and hose E. coli ribosome translation. These DNA regions have been examined for sequence-directed DNA bending. First, plasmids containing a tandem repeat of e...

Just upstream of the gene 10 in bacteriophage T7 genome there exist expression regulatory sequences for phage T7 RNA polymerase transcription and hose E. coli ribosome translation. These DNA regions have been examined for sequence-directed DNA bending. First, plasmids containing a tandem repeat of each were constructed. Then, each plasmid was digested with various restriction enzymes to produce circularly permuting DNA fragments of the same length. Our results of polyacrylamide gel electrophoresis of the fragments revealed that about 30 base pairs downstream of the translation initiation codon ATG there appears to exist a sequence-directed DNA bending. The bent region does not contain any oligo(A) tract which is known to cause bending of DNA. Calculations of the accumulated DNA axis deflection angles of DNA fragments based on the roll and tilt wedge angles of nearest neighbor base pairs revealed a good correlation between the experimental gel mobility retardation degrees and calculated DNA axis deflection angles of all the permuted DNA fragments. These data support the wedge model of DNA curvature.