초록

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Soybean and tobacco tissue cultured with modified MS media containing 4 different ratio(as N) of nitrate to ammonium combination which were 3:0, 2:1, 1:2 and 0:3. The highest callus growth in soybean were observed in the 2:1 medium. The medium containing nitrate only was detrimental to soybean callu...

Soybean and tobacco tissue cultured with modified MS media containing 4 different ratio(as N) of nitrate to ammonium combination which were 3:0, 2:1, 1:2 and 0:3. The highest callus growth in soybean were observed in the 2:1 medium. The medium containing nitrate only was detrimental to soybean callus growth. Tobacco callus grown with nitrate-only grew as well as those in the 2:1 and very slowly with ammonium-only. In tobacco callus, the total nitrogen in the callus increased with the increase of nitrogen concentration in the medium, but in soybean callus, the opposite result was noted. Nitrate reductase activity in tobacco callus was high when grown with nitrate-only but low with ammonium-only. In case of soybean callus, nitrate reductase activity was high in the 2:1 and remarkably low in nitrate-only medium. Both in soybean and tobacco callus, the activity of glutamine synthetase was high with nitrate-only, but low with ammonium.

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