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제안 방법
- microscope. Abnormal morphology obser ved in this experiment was shrunken cytoplasm, plasmolysis from the cell wall, malformation or irregular cell shape, accumulation of lipid gran ules, and retrogression of mitochondrial cristae (Fig. 3). Rho (1992) and Kim (1993) have repor ted deformity of root tip cells of receptor plants by aqueous extracts.
- Alternate germination testing was performed in which two sheets of filter paper covering the bottom of a Petri dish were moistened with 8 mL distilled water and 50 seeds of Amaranthus mangostanus were sown. A small aluminum vial (diameter 5 mm, height 5 mm) was put in the center of the Petri dish containing either 5 μL, 10 μL, 15 μL, or 20 μ L of the essential oil of C.
- Then 80 mm silver sections were made using an LKB-V type ultramicrotome. Silver sections were collected on a copper grid (100 mesh) and double-dyed with uranyl acetate for 30 minutes and lead citrate for 10 minutes then examined by transmission electron microscope (Hitachi H-600, 75 KV).
- An empty aluminum vial was used as the control. Six days after sowing, seed germination and seedling length of test plants were evaluated.
- To ascertain whether the volatile substances of C. obtusa leaves could be hydrated by water, 30, 50, and 70 grams of C. obtusa leaves were placed in 1,700 mL containers with 100 mL beakers half full of distilled water and sealed with vinyl wrap. After three days, the distilled water from the beakers was used to moisten the filter paper for a germination test as previously described.
이론/모형
- The essential oil of C. obtusa was obtained by Yun's method employing the Karlsruker's apparatus (Stahl 1973).
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