[논문]The Role of Neuropeptide Y in the Central Regulation of Grass Intake in Sheep

Sunagawa, K.; Weisiger, R.S.; McKinley, M.J.; Purcell, B.S.; Thomson, C.; Burns, P.L.

doi:10.5713/ajas.2001.35

[국내논문] The Role of Neuropeptide Y in the Central Regulation of Grass Intake in Sheep 원문보기

Sunagawa, K. (Department of Animal Physiology, Faculty of Agriculture, University of the Ryukyus) , Weisiger, R.S. (Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne) , McKinley, M.J. (Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne) , Purcell, B.S. (Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne) , Thomson, C. (Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne) , Burns, P.L. (Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne)

The physiological role of brain neuropeptide Y (NPY) in the central regulation of grass intake in sheep was investigated through a continuous intracerebroventricular (ICV) infusion of NPY at a dose of $5{\mu}g/0.2ml/hr$ for 98.5 hours from day 1 to day 5. Sheep (n=5) were fed for 2 hours once a day, and water and 0.5 M NaCl solution were given ad libitum. Feed intake during ICV NPY infusion increased significantly compared to that during ICV artificial cerebrospinal fluid (CSF) infusion. Water and NaCl intake during ICV NPY infusion remained unchanged. Mean arterial blood pressure (MAP) and plasma osmolality during ICV NPY infusion were not significantly different from those during ICV CSF infusion. On the other hand, plasma glucose concentration during ICV NPY infusion increased significantly compared to that during ICV CSF infusion. The results suggest that brain NPY acts as a hunger factor in brain mechanisms controlling feeding to increase grass intake in sheep.

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문제 정의

The aim of the present research was to investigate the role of brain NPY in the central regulation of grass intake in sheep. We gave a continuous ICV infusion of the peptide at a very small dose of 5 “g /0.

가설 설정

On the other hand, feed intake during ICV NPY infusion on days 3, 4 and 5 increased significantly compared to that during ICV CSF infusion. The results of water intake are shown in table 3. Water intake during ICV CSF infusion was not significantly different from that during non-ICV infusion. Water intake during ICV NPY infusion was also unchanged from that during ICV CSF infusion.

제안 방법

The alfalfa chaff was ground by Willey milling machine (Type 40-525P, Ikemoto Rika Kougyou, Japan) and the chemical composition was analyzed (Kato, 1988), The digestible crude protein (DCP) and the total digestible nutrients (TDN) were calculated using the chemical composition and digestibility (table 1). The digestibility of the feed was determined using the in vivo method by the formula the digestibility of feed (%)=(feed intake-feaces)/feed intake x 100.

대상 데이터

Five crossbred Merino ewes, 35 ~ 48 kg body weight, were used. The sheep were ovariectomized and had both carotid arteries exteriorized in skin loop.
Five crossbred Merino ewes, 35 ~ 48 kg body weight, were used. The sheep were ovariectomized and had both carotid arteries exteriorized in skin loop.
The mean arterial blood pressure was measured from the carotid artery via a heparin-saline filled 18 gauge needle and polyethylene tube hooked up to a COBE disposable transducer model 345-931-009. The pressure recording system was a JRACK (Australia) rack type RK8 pressure amplifier with a GRAPHTEC Thermal Arraycorder model WR7700. The blood samples were taken via this cannula from a carotid artery.
Bastias is gratefully acknowleged. We also thank G. Mcllvride, M. Miyazato, K. Fujisawa, I. Nagamine and B. W. H. E. Prasetiyono for help in preparing the manuscript, and Dr. K. Hikosaka for proofreading. This work was supported by a block grant to Howard Florey Institute of Experimental Physiology and Medicine by the National Health and Medical Research Council of Australia.

데이터처리

A one-way classification and subsequent Duncan's Multiple Range Tests were used to compare the data during non-ICV infusion, ICV CSF infusion and ICV NPY infusion. For statistical analysis, GLM procedures (SAS, 1990) were adopted.

이론/모형

A one-way classification and subsequent Duncan's Multiple Range Tests were used to compare the data during non-ICV infusion, ICV CSF infusion and ICV NPY infusion.

성능/효과

The results of feed intake are shown in table 2. Feed intake during ICV CSF infusion was not significantly different from that during non-ICV infusion and was not influenced by the continuous 98.5 hour infusion. On the other hand, feed intake during ICV NPY infusion on days 3, 4 and 5 increased significantly compared to that during ICV CSF infusion.
Water intake during ICV NPY infusion was also unchanged from that during ICV CSF infusion. The results of salt intake are shown in table 4. NaCl intake during ICV CSF infusion was not significantly d迁ferent from that during non-ICV infusion. NaCl intake during NPY ICV infusion was also unchanged from that during ICV CSF infusion.

후속연구

However, the physiological mechanisms regulating feed intake in ruminants are still largely unclear. In order to clarify these mechanisms, further research is necessary on the brain mechanisms controlling feed intake including how the brain processes peripheral information. ICV bolus injection of 10 // g of NPY in feed-satiated sheep increased feed intake of concentrate pellets, while an intravenous injection produced no effect (Miner, 1992).

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[국내논문] The Role of Neuropeptide Y in the Central Regulation of Grass Intake in Sheep 원문보기

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