HMG-CoA Reductase의 저해제 탐색과 가금의 콜레스테를 저하 효과 Screening of 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Inhibitors In Vitro and Its Application to Pullets원문보기
Moon, Young-Ja
(Department of Food and Nutrition, Woosong Information College)
,
Yeom, Keum-Hee
(Department of Food Science and Technology, Chung Nam National University)
,
Sung, Chang-Keun
(Department of Food Science and Technology, Chung Nam National University)
체내 cholesterol 수치를 낮추기 위하여 HMG-CoA reductase 활성을 저해하는 물질들을 닭에게 급여하여 in vitro상에서 검토한 결과, HMG-CoA reductase 활성에 대한 마늘, lovastatin과 copper의 순으로 저해능은 51.3%, 87.5%, 82.0%이었다. 또한 혈장, 가슴조직, 간장의 cholesterol 함량에 대한 마늘(3% in diet), lovastatin(300mg/Kg of diet), copper(200mg/Kg of diet)의 효과를 검토하였다. 혈장 중의 cholesterol 함량과 HDL, LDL cholesterol 함량은 3% 마늘의 투여로 감소되어졌으나 copper의 투여는 오히려 증가하였고, lovastatin 투여에 의한 차이는 나타나지 않았다. 그리고 간장과 가슴조직의 cholesterol과 triglyceride는 사료에 cholestrol의 수치를 저하시키는 물질을 첨가하였으나 별다른 차이가 없었다.
체내 cholesterol 수치를 낮추기 위하여 HMG-CoA reductase 활성을 저해하는 물질들을 닭에게 급여하여 in vitro상에서 검토한 결과, HMG-CoA reductase 활성에 대한 마늘, lovastatin과 copper의 순으로 저해능은 51.3%, 87.5%, 82.0%이었다. 또한 혈장, 가슴조직, 간장의 cholesterol 함량에 대한 마늘(3% in diet), lovastatin(300mg/Kg of diet), copper(200mg/Kg of diet)의 효과를 검토하였다. 혈장 중의 cholesterol 함량과 HDL, LDL cholesterol 함량은 3% 마늘의 투여로 감소되어졌으나 copper의 투여는 오히려 증가하였고, lovastatin 투여에 의한 차이는 나타나지 않았다. 그리고 간장과 가슴조직의 cholesterol과 triglyceride는 사료에 cholestrol의 수치를 저하시키는 물질을 첨가하였으나 별다른 차이가 없었다.
The primary objective of these studies was to screen the materials showing inhibitions of HMG-CoA reductase in vitro. The secondary objective was to determine the effect of garlic, lovastatin and copper on cholesterol concentrations in plasma, liver and breast tissues in pullets. The degree of inhib...
The primary objective of these studies was to screen the materials showing inhibitions of HMG-CoA reductase in vitro. The secondary objective was to determine the effect of garlic, lovastatin and copper on cholesterol concentrations in plasma, liver and breast tissues in pullets. The degree of inhibition of the selective samples on HMG-CoA reductase activity was determined in vitro. The inhibition ratios of water soluble garlic extracts, lovastatin (methanol extracts) and copper to HMG-CoA reductase activity were 51.3%, 87.5%, and 82.0%, respectively. Control diet (basal diet) and experimental diets, garlic powder (3% in diet), lovastatin (300mg/Kg of diet) and copper (200mg/Kg of diet) were fed to pullets in order to investigate the changes of cholesterol concentration in plasma and tissues. Total cholesterol, HDL- and LDL-cholesterol in blood plasma were significantly reduced in pullets fed diet containing 3% garlic powder. However, copper significantly increased total cholesterol compared to control and lovastatin did not affect plasma cholesterol concentration. Total cholesterol and triglyceride of liver and breast tissues in pullets were not affected by adding the cholesterol-lowering materials to diets. The data suggests that it is not easy for HMG-CoA reductase inhibitors to reduce cholesterol levels in body due to complication of cholesterol metabolism. However, garlic administration can lower the levels of plasma cholesterol in pullets.
The primary objective of these studies was to screen the materials showing inhibitions of HMG-CoA reductase in vitro. The secondary objective was to determine the effect of garlic, lovastatin and copper on cholesterol concentrations in plasma, liver and breast tissues in pullets. The degree of inhibition of the selective samples on HMG-CoA reductase activity was determined in vitro. The inhibition ratios of water soluble garlic extracts, lovastatin (methanol extracts) and copper to HMG-CoA reductase activity were 51.3%, 87.5%, and 82.0%, respectively. Control diet (basal diet) and experimental diets, garlic powder (3% in diet), lovastatin (300mg/Kg of diet) and copper (200mg/Kg of diet) were fed to pullets in order to investigate the changes of cholesterol concentration in plasma and tissues. Total cholesterol, HDL- and LDL-cholesterol in blood plasma were significantly reduced in pullets fed diet containing 3% garlic powder. However, copper significantly increased total cholesterol compared to control and lovastatin did not affect plasma cholesterol concentration. Total cholesterol and triglyceride of liver and breast tissues in pullets were not affected by adding the cholesterol-lowering materials to diets. The data suggests that it is not easy for HMG-CoA reductase inhibitors to reduce cholesterol levels in body due to complication of cholesterol metabolism. However, garlic administration can lower the levels of plasma cholesterol in pullets.
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문제 정의
The objective of these studies was to screen the materials showing the inhibitions of HMG-CoA reductase in vitro. The secondary objective was to determine the effects of garlic, lovastatin and copper on cholesterol concentrations in plasma, liver and breast tissues in pullets.
가설 설정
a,bMeans having same superscripts do not significantly differ (P<0.05).
제안 방법
The objective of these studies was to screen the materials showing the inhibitions of HMG-CoA reductase in vitro. The secondary objective was to determine the effects of garlic, lovastatin and copper on cholesterol concentrations in plasma, liver and breast tissues in pullets.
0%이었다. 또한 혈장, 가슴조직, 간장의 cholesterol 함량에 대한 마늘(3% in diet), lova- statin(300mg/Kg of diet), copper(200mg/Kg of diet)의 효과를 검토하였다. 혈장 중의 cholesterol 함량과 HDL, LDL 산lolesterol 함량은 3% 마늘의 투여로 감소되어졌으나 copper의 투여는 오히려 증가하였고, lovastatin 투여에 의한 차이는 나타나지 않았다.
대상 데이터
Field study was conducted with commercial 13-wk-old ISA Brown pullets. The hens were alloted to individual wire-floored metabolism cages (one hen per cage) and subjected to four dietary treatments : 1) control 2) 3% garlic 3) 300mg lovastatin/Kg 4) 200mg copper/Kg.
Ten pullets were involved for the each experimental work, including control. Three times were repeated for analysis, therefore totally one hundred twenty pullets were employed for this work.
데이터처리
Data obtained from blood were subjected to analysis of covariance using the General Linear Model procedure of SASr11) and comparisons of means between treatment were conducted by Student Newman Keuls test(2) when significant difference (P<.05) was fb니nd. Data obtained from liver and breast were analysed by one-way analysis of variance test.
05) was fb니nd. Data obtained from liver and breast were analysed by one-way analysis of variance test. Statistical significances among treatment means were determined by the method of new multiple range test of Duncan13> when the F value was significant at 5% level.
이론/모형
4 ineluding 2mM DTT (Dithiothreitol) and stored at —70 ℃ prior to assay for enzymatic activity. Protein concentration was determined by the method of Lowly et ale\ Inhibition activitiy against HMG-CoA reductase was measured using a modified* method of Hulcher et al.9). Microsomal protein Img, HMG-CoA 150nM, NADP 2μ M, Glucose-6-phosphate 3μM, Glucose-6-phosphate dehydrogenase 2 units and Test samples (lOOmg/ml) lOOpl were mixed, reacted at 37 ℃ for 30 min and added lOmM, sodium arsenite 20pl.
Samples were extracted with either methanol or water, and stored at 4 ℃ before enzyme assay using Lee et aL's method”. Selected materials are shown in Table 1.
성능/효과
The results of our experiments demonstrated that under normal dietary conditions, pullet may be capable of synthesizing cholesterol in excess of its needs for preparing yolk deposition.
It is reported that lovastatin has the pharmacological_ characteristics in reducing cholesterol levels by inhibiting the activtiy of HMG-CoA reductase and stimulating receptor-mediated uptake and degradation of LDL cholesterol in the liver6*. The results of the present study show that lovastatin inhibits the activity of HMG-CoA reductase in vitro (Table 3).
체내 cholesterol 수치를 낮추기 위하여 HMG-CoA reductase 활성을 저해하는 물질들을 닭에게 급여하여 in vitro상에서 검토한 결과, HMG-CoA reductase 활성에 대한 마늘, lovastatin과 copper의 순으로 저해능은 51.3%, 87.5%, 82.0%이었다. 또한 혈장, 가슴조직, 간장의 cholesterol 함량에 대한 마늘(3% in diet), lova- statin(300mg/Kg of diet), copper(200mg/Kg of diet)의 효과를 검토하였다.
또한 혈장, 가슴조직, 간장의 cholesterol 함량에 대한 마늘(3% in diet), lova- statin(300mg/Kg of diet), copper(200mg/Kg of diet)의 효과를 검토하였다. 혈장 중의 cholesterol 함량과 HDL, LDL 산lolesterol 함량은 3% 마늘의 투여로 감소되어졌으나 copper의 투여는 오히려 증가하였고, lovastatin 투여에 의한 차이는 나타나지 않았다. 그리고 간장과 가슴조직의 cholesterol과 triglyceride는 사료에 cholestrol의 수치를 저하시키는 물질을 첨가하였으나 별다른 차이가 없었다.
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