The Viability Change of Yeast Cell Responding to Trehalose Accumulation and Maintaining Neutral Trehalase Activity under Extracellular pH Acidified by $H_2SO_4$원문보기
Saccharomyces cerevisiae KNU5377 (KNU5377) and S. cerevisiae ATCC24858 (ATCC24858) were exposed to $H_2SO_4$ as a stress, which was added at various concentrations to a YPD media. The growth of KNU5377 was reduced to approximately 60% in the YPD media containing 40 nm sulfuric acid when c...
Saccharomyces cerevisiae KNU5377 (KNU5377) and S. cerevisiae ATCC24858 (ATCC24858) were exposed to $H_2SO_4$ as a stress, which was added at various concentrations to a YPD media. The growth of KNU5377 was reduced to approximately 60% in the YPD media containing 40 nm sulfuric acid when compared to the non-stressed condition. When their growth was monitored during an overnight culture, two strains, KNU5377 and ATCC24858, could not grow when exposed to over 50 mM of sulfuric acid. After a short exposure to this acid for 1 h, KNU5377 exhibited stronger resistance against $H_2SO_4$ than ATCC24858. The neutral trehalase activity of KNU5377 unchanged despite under various concentrations of $H_2SO_4$. In contrast, It at of ATCC24858 was much low at higher $H_2SO_4$concentrations. Trehalose, a non-reducing disaccharide, was maximally accumulated after a short exposure to 60 nm $H_2SO_4$ for KNU5377, but it was reduced under more severe stressful conditions. These results suggest that KNU5377 should modulate the trehalose concentrations under the severe stress condition of high sulfuric acid concentrations. The most highly induced protein in the KNU5377 exposed to sulfuric acid was found to be an approximately 23 kDa protein, which was revealed to be the 605 large subunit ribosomal protein, Ll3 by FASTA search results.
Saccharomyces cerevisiae KNU5377 (KNU5377) and S. cerevisiae ATCC24858 (ATCC24858) were exposed to $H_2SO_4$ as a stress, which was added at various concentrations to a YPD media. The growth of KNU5377 was reduced to approximately 60% in the YPD media containing 40 nm sulfuric acid when compared to the non-stressed condition. When their growth was monitored during an overnight culture, two strains, KNU5377 and ATCC24858, could not grow when exposed to over 50 mM of sulfuric acid. After a short exposure to this acid for 1 h, KNU5377 exhibited stronger resistance against $H_2SO_4$ than ATCC24858. The neutral trehalase activity of KNU5377 unchanged despite under various concentrations of $H_2SO_4$. In contrast, It at of ATCC24858 was much low at higher $H_2SO_4$concentrations. Trehalose, a non-reducing disaccharide, was maximally accumulated after a short exposure to 60 nm $H_2SO_4$ for KNU5377, but it was reduced under more severe stressful conditions. These results suggest that KNU5377 should modulate the trehalose concentrations under the severe stress condition of high sulfuric acid concentrations. The most highly induced protein in the KNU5377 exposed to sulfuric acid was found to be an approximately 23 kDa protein, which was revealed to be the 605 large subunit ribosomal protein, Ll3 by FASTA search results.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
In this work, the change in trehalose accumulation and the activity of the trehalose hydrolyzing enzyme, neutral trehalase, and the comparison of the resistance under various H2SO4 concentrations was investigated both in this strain Saccharomyces cerevisiae KNU5377 and a reference strain S. cerevisiae ATCC24858. In addition, the amino acid se quencing data of a 23 kDa protein, which was highly ex pressed after exposure to sulfate, was also reported.
성능/효과
However, their function as a structural constituent of a ribosome may be to endow the cells with more effective protein synthesis under stressful conditions. In conclusion, the 160 and 90 kDa proteins as well as this amino acid sequenced 23 kDa protein should be further investigated in order to determine their exact role in the yeast cell, Saccharomyces cerevisiae KNU5377.
cerevisiae KNU5377 (lane 6 to 10) were exposed to 0 rr VI (lane 1, 6), 30 mM (lane 2, 7), 90 mM (lane 3, 8), 150 rr Vf (lane 4, 9) and 300 mM (lane 5, 10) H2SO4. It showed tl it the approximately 90, 70, 60, 40 and 23 kDa proteins were ir luced in both yeast cells, but the 160 kDa protein (upper ai gw) was only expressed in S. cerevisiae KNU5377.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.