[논문]Loss-of-function and Gain-of-function Rice Mutants from Gamma-Ray Mutagenesis

Lee, Seon-Woo; Park, Gyung-Ja; Kim, Jin-Cheol; Kim, Heung-Tae; Park, Yong-Ho; Cho, Kwang-Yun

doi:10.5423/ppj.2003.19.6.301

Loss-of-function and Gain-of-function Rice Mutants from Gamma-Ray Mutagenesis 원문보기

The plant pathology journal, v.19 no.6, 2003년, pp.301 - 304

Lee, Seon-Woo (Biological Function Research Team, Korea Research Institute of Chemical Technology) , Park, Gyung-Ja (Biological Function Research Team, Korea Research Institute of Chemical Technology) , Kim, Jin-Cheol (Biological Function Research Team, Korea Research Institute of Chemical Technology) , Kim, Heung-Tae (Department of Agricultural Biology, Chungbuk National University) , Park, Yong-Ho (Biological Function Research Team, Korea Research Institute of Chemical Technology) , Cho, Kwang-Yun (Biological Function Research Team, Korea Research Institute of Chemical Technology)

Abstract ▼ AI-Helper

Gamma-ray irradiation is known to induce various mutations in plants caused by chromosome alterations. This study investigated disease responses of selected gamma-ray induced rice mutants generated from seven Japonica-type rice cultivars against three plant diseases. Among the tested 22 mutants, three gain-of-function mutants and six loss-of-function mutants against rice blast were obtained, as well as three loss-of-function mutants against bacterial leaf blight (BLB). Two of the loss-of-function mutants were susceptible to both rice blast and BLB. Gain-of-function mutation has not been frequently observed in rice plants, thus, the mutants can be used to identify loci of novel genes for the regulation of disease resistant response.

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문제 정의

It is expected that gamma-ray mutagenesis could cause many variations in the disease responses of rice plants. Therefore, this study investigated the alterations in disease responses of rice gamma-ray mutants using the above-mentioned three plant diseases. Gamma-ray irradiated mutants from seven Japonica- type rice cultivars were previously generated at the Korea Atomic Energy Research Institute.
This study described rice mutants generated from gamma ray irradiation with altered disease responses, and suggested that gamma-ray mutants could be important materials for rice genetic study to identify novel genetic loci involved in disease resistance signaling. Gamma-ray mutagenesis would also be a good alternative for functional genomic study in rice to supplement the T-DNA tagging mutagenesis.

제안 방법

, 1973). Disease incidence was scored by measuring the length of leaf blight lesion after maintaining the plants in an 80% relative humidity room for 5 days.
Disease development was apparent by the lesion formation and leaf tissue collapse. Finally, the percentage of infected leaf area and infected sheath area were determined to estimate the responses of rice cultivars to rice blast and RSB, respectively. Bacterial inoculum for X.
Mutants with alteration in quantitative traits were selected and several generations were created to fix the mutated phenotypes. In this study, seven wild type cultivars and their 22 selected mutants were used to evaluate the changes in the response to three plant diseases.
oryzae pv. oryzaeKXO85, and a strain of C. sasaki were used in this study to inoculate the rice plants as pathogens causing rice blast, BLB and RSB, respectively.

대상 데이터

Rice cultivars (Oryza sativa L.) used in this study are all Japonica-type cultivars, such as Hwaseongbyeo, Chucheongbyeo, Seomjinbyeo, Suwon 345, Ilpumbyeo, Jakwangdo, Ybngkeumbyeo, and Nagdongbyeo. Disease responses of these cultivars are variable according to diseases including rice blast and BLB, while all of them are susceptible to RSB.

참고문헌 (16)

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Loss-of-function and Gain-of-function Rice Mutants from Gamma-Ray Mutagenesis 원문보기

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Loss-of-function and Gain-of-function Rice Mutants from Gamma-Ray Mutagenesis 원문보기

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