[논문]합성화학물질들의 유전독성평가(Ⅸ) -합성 제초제 Pretilachlor -

류재천; 김연정

합성화학물질들의 유전독성평가(Ⅸ) -합성 제초제 Pretilachlor -
Evaluation of the Genetic Toxicity of Synthetic Chemicals (Ⅸ) a Synthetic Selective Herbicide, Pretilachlor- 원문보기

환경독성학회지 = Journal of environmental toxicology, v.19 no.1, 2004년, pp.93 - 100

류재천 (한국과학기술연구원, 생체대사연구센터, 독성연구실) , 김연정 (한국과학기술연구원, 생체대사연구센터, 독성연구실)

초록
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Pretilachlor [2-chloro-N -(2, 6-diethylphenyl)-N-(2-propoxyethyl) acetamide, $C_{17}$H$_{26}$ClNo$_2$, M.W.=311.9, CAS No.51218-49-6]는 제초제의 일종으로 본 연구에서는 박테리아 복귀 돌연변이 시험과 포유동물 세포를 이용한 염색체 이상 시험 및 마우스를 이용한 in vivo소핵 시험을 수행하여 pretilachlor의 유전독성을 평가하였다. 박테리아 복귀 돌연변이 시험에서 pretilachlor는 Salmonella typhimurium TA98, TA 100, TA1535, TA1537 균주의 대사 활성계 존재 및 부재시 313-5,000$\mu\textrm{g}$/plate의 범위에서 농도 의존적인 돌연변이 율의 증가를 관찰할 수 없었다. 또한 포유동물 세포인 Chinese hamster lung (CHL) fibroblast를 이용한 염색체 이상 시험에서 pretilachlor는 대사 활성계 존재 및 부재시 1.56-6.24$\mu\textrm{g}$/mL의 농도에서 clastogenicity를 보이지 않았고, 137.5-550.1 mg/kg의 pretilachlor를 복강 주사한 마우스의 골수세포를 이용한 in vivo소핵 시험의 결과에서도 통계적으로 유의한 소핵 유발능을 관찰할 수 없었다었다

주제어

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제안 방법

For the aberration assay, three different doses, including the IC50 value as a maximum dose, were prepared and separately added to 3-day-old cultures (approximat이y 105 cells/60 mm dish). In the absence of metabolic activation, cultures were treated for 24 hours with the test article, while in the presence of metabolic activation, cells were treated for 6 hours because of toxicity of S-9 and then maintained for 18 hours in the fresh medium to adjust a time equiv alent to about 1.
Outbred mice of strain ICR, 7~8 weeks old, were used in this study. The mice were allowed an adapta tion period for 1 week, then randomized and subject ed to the study. The six animals were housed for each group.
To elucidate the genotoxicity of pretilachlor, we performed bacterial gene mutation, in vitro chromo some aberration and in vivo micronucleus assay in this study.

대상 데이터

Outbred mice of strain ICR, 7~8 weeks old, were used in this study. The mice were allowed an adapta tion period for 1 week, then randomized and subject ed to the study.
The mice were allowed an adapta tion period for 1 week, then randomized and subject ed to the study. The six animals were housed for each group. The test article was applied intraperitoneally in three doses in volumes of 10 mL/kg.

이론/모형

Breaks less than the width of a chromatid were designated as gaps in our criteria, and not included as chromosomal aberration. Aberra tion frequencies, defined as aberrations observed divided by number of cells counted, were analyzed using Fishers exact test (Altman, 1993) with Dunnetts adjustment and compared with results from the sol vent controls. Therefore, dose-dependent responses and the statistical significance in /?-value will be considered as positive results in our judgement.
The mutagenic potential of pretilachlor was inves tigated in the Salmonella typhimurium microsomal activation assay. This assay detects materials that cause specific point mutations such as base-pair substitution and frameshift mutation in different S.

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저자의 다른 논문 :

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