The current study examined the effects of freeze-dried mulberry fruit on disaccharidase activity in the small intestine and the lowering of blood glucose in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats were randomly assigned to one normal and three streptozotocin (STZ)-induce...
The current study examined the effects of freeze-dried mulberry fruit on disaccharidase activity in the small intestine and the lowering of blood glucose in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats were randomly assigned to one normal and three streptozotocin (STZ)-induced diabetic groups. The diabetic groups were fed a mulberry fruit-free diet (DM-group), 0.3% mulberry fruit diet (DM-F group) or 0.6% mulberry fruit diet (DM-2F group). After they were fed the experimental diets for three weeks, diabetes was induced with an intraperitoneal injection of streptozotocin 50 mg/kg b.w before sacrificing 9 days later using the same experimental treatments. Analyses of anthocyanins, flavonoid and 1-deoxynojirimycin (DNJ) of lyophilized mulberry fruit were carried out and the major anthocyanins were rutin (142.5 mg), isoquercitrin (10.3 mg), quercetin (5.8 mg), morin (1.6 mg) dihydroquercetin (3.83 mg), cy-3-O-glucopyranoside (230.45 mg) and cy-3-O-rutinoside (131.5 mg) on the basis of 100 g dry weight. Total DNJ content was 2.39 mg/g dry weight of lyophilized mulberry fruit. Blood glucose level decreased in the diabetic mts fed the mulberry fruit supplement. The content of the liver glycogen increased in the diabetic mts fed the mulberry fruit supplement. Disaccharidase activity in the proximal part of the intestine, such as that of maltase, sucrase and lactase in the mulberry fruit supplementation groups, were lower than that of the DM group. These results suggest that mulberry fruit possess a suppressive effect on hyperglycemia, possibly by inhibiting the activity of disaccharidase in the small intestine of rats.
The current study examined the effects of freeze-dried mulberry fruit on disaccharidase activity in the small intestine and the lowering of blood glucose in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats were randomly assigned to one normal and three streptozotocin (STZ)-induced diabetic groups. The diabetic groups were fed a mulberry fruit-free diet (DM-group), 0.3% mulberry fruit diet (DM-F group) or 0.6% mulberry fruit diet (DM-2F group). After they were fed the experimental diets for three weeks, diabetes was induced with an intraperitoneal injection of streptozotocin 50 mg/kg b.w before sacrificing 9 days later using the same experimental treatments. Analyses of anthocyanins, flavonoid and 1-deoxynojirimycin (DNJ) of lyophilized mulberry fruit were carried out and the major anthocyanins were rutin (142.5 mg), isoquercitrin (10.3 mg), quercetin (5.8 mg), morin (1.6 mg) dihydroquercetin (3.83 mg), cy-3-O-glucopyranoside (230.45 mg) and cy-3-O-rutinoside (131.5 mg) on the basis of 100 g dry weight. Total DNJ content was 2.39 mg/g dry weight of lyophilized mulberry fruit. Blood glucose level decreased in the diabetic mts fed the mulberry fruit supplement. The content of the liver glycogen increased in the diabetic mts fed the mulberry fruit supplement. Disaccharidase activity in the proximal part of the intestine, such as that of maltase, sucrase and lactase in the mulberry fruit supplementation groups, were lower than that of the DM group. These results suggest that mulberry fruit possess a suppressive effect on hyperglycemia, possibly by inhibiting the activity of disaccharidase in the small intestine of rats.
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문제 정의
The purpose of this study was to investigate the effects of mulberry fruit on disaccharidase activity in the small intestine and the lowering of blood glucose in diabetes-induced experimental rats.
제안 방법
The first 3 mL was discarded and the next 1 mL was used for HPLC analysis for quantification of flavonoids of mulberry fruit. HPLC analysis was performed on the same HPLC equipment and conditions for anthocyanin analysis, with the exception of a mobile phase [gradient elution from solvent A (4.5% formic acid in H2O) to solvent B (90% aq. CH3CN) for 60 min] and UV detection at 280 (dihydroquercetin) and 360 nm (four flavonoids except for dihydroquercetin). Quantification of flavonoids was performed similarly in the case of anthocyanins.
45 gm membrane filter (Gelman, USA) and injected in HPLC for quantification of mulberry fruit anthocyanins. HPLC analysis was performed using a Gilson 506B HPLC system equipped with 170 UV-vis detector, Gilson Unipoint™ 3.0 software and 23IXL autosampler with a 10 J11 loop, using a YMC-Pack Pro Cis column (5 Jim, 4.6 LD.x250 mm, YMC Inc., USA) with a mobile phase of 1.0% H3PO4 in CH3CN-H2O-HOAc (7.5:85.5:7, v/v) at a flow rate of LOmL/min with a UV detector at 520 nm. Individual anthocyanins were identified by a comparison of their retention time with those of the two standard anthocyanins [cyanidin-3-O-P-D-glucoside (anthocyanin 1), cyanidin-3-(9-P-D-rutinoside (anthocyanin 2)] isolated from a previous report (21).
대상 데이터
The Cheongil mulberry fruit that was used in this experiment was grown in the fields of the Youngcheon Silkworm Culture Agricultural Co-operative Association, Youngcheon, Korea and was harvested in early June 2003. All of the mulberry fruit that was used underwent abrasion to 100 mesh size after being freeze-dried for the experiment.
(Samyang, Seoul, Korea) for 7 days after arrival. They were randomly divided into one normal group and three diabetic groups of 10 experimental rats each. The four groups were fed experimental diets for 3 weeks and the diabetic groups were given a mulberry fruit-free diet (DM group), 0.
데이터처리
All data were assessed by analysis of variance (ANOVA). If significance was found by ANOVA, comparisons among group means were made using Tukey's Honestly Significant Difference test.
999 for each anthocyanin. The calibration lines (y=10433x-30.296 for anthocyanin 1, y=5346.7x-0.4025 for anthocyanin 2) used were determined using a least squares regression method. The concentration of anthocyanins were determined using the calibration curves of two standard anthocyanins, and expressed as mg% of dried weight.
성능/효과
index are shown in Table 3. Body weights significantly decreased in the diabetic groups as compared to the nonnal group, but the difference among the diabetic groups was not significant Intestine weight and length were not significantly different among the diabetic groups. The small intestine index in the diabetic groups was significantly higher than that of the normal group but the effect of the mulberry fruits was not observed.
The content of glycogen (Fig. 2) in the DM group decreased 27% compared to that of the normal group, but those of the DM-F and DM-2F groups increased 21% and 18%, respectively, compared to that of the DM group.
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